Restriction map for
pFN21AE1333
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AspI AvaI
AviII BalI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlpI Bpu1102I BpuAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
Hin1I HindIII HpaI HpaII KasI
KspI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwNI 1584 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 898 ATTAAT AT^TAAT
AsnI 898 ATTAAT AT^TAAT
AspHI 1645 GTGCTC G[AT]GC[AT]^C
AvrII 940 CCTAGG C^CTAGG
BbrPI 1239 CACGTG CAC^GTG
BglI 242 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 940 CCTAGG C^CTAGG
BsaBI 1489 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1645 GTGCTC G[AT]GC[AT]^C
BspHI 1197 TCATGA T^CATGA
BspMI 238 ACCTGC ACCTGC(4/8)
BstXI 1227 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 259 AGATCC [GA]^GATC[CT]
CfoI 1760 GCGC GCG^C
DraIII 503 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1299 CCATGG C^C[GA][CT]GG
EcoNI 62 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 409 GAATTC G^AATTC
FauI 614 CCCGC CCCGC(4/6)
HgaI 276 GACGC GACGC(5/10)
HhaI 1760 GCGC GCG^C
HinP1I 1760 GCGC G^CGC
HincII 195 GTTGAC GT[CT]^[GA]AC
HindII 195 GTTGAC GT[CT]^[GA]AC
MamI 1489 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 144 CAATTG C^AATTG
MflI 259 AGATCC [GA]^GATC[CT]
MunI 144 CAATTG C^AATTG
MwoI 242 GCCTCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1299 CCATGG C^CATGG
NspI 918 ACATGC [GA]CATG^[CT]
PmaCI 1239 CACGTG CAC^GTG
PmlI 1239 CACGTG CAC^GTG
PshBI 898 ATTAAT AT^TAAT
PstI 1401 CTGCAG CTGCA^G
RcaI 1197 TCATGA T^CATGA
SapI 28 GCTCTTC GCTCTTC(1/4)
SexAI 1588 ACCTGGT A^CC[AT]GGT
SnaBI 356 TACGTA TAC^GTA
SpeI 451 ACTAGT A^CTAGT
XhoII 259 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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Acc65I 986 GGTACC G^GTACC
1592 GGTACC
AfaI 987 GTAC GT^AC
1593 GTAC
Asp718I 986 GGTACC G^GTACC
1592 GGTACC
BanII 26 GGGCTC G[GA]GC[CT]^C
883 GAGCCC
BsaAI 356 TACGTA [CT]AC^GT[GA]
1239 CACGTG
Cac8I 129 GCCTGC GC[ACGT]^[ACGT]GC
719 GCAAGC
KpnI 986 GGTACC GGTAC^C
1592 GGTACC
PflMI 68 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1258 CCATCATGTGG
PvuII 414 CAGCTG CAG^CTG
603 CAGCTG
RsaI 987 GTAC GT^AC
1593 GTAC
SfaNI 335 GATGC GCATC(5/9)
844 GATGC
TfiI 861 GAATC G^A[AT]TC
1534 GATTC
Van91I 68 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1258 CCATCATGTGG
XcmI 916 CCACATGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1527 CCAATGTGATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanI BbvI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfcI StyI
Tru9I TseI Tsp45I Tsp509I TspRI