Restriction map for
pFN21AE6224
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoT22I
FauI FbaI Fnu4HI FseI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII ItaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TseI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AflIII 571 ACATGT A^C[GA][CT]GT
AluI 683 AGCT AG^CT
AlwI 613 GATCC GGATC(4/5)
ApaI 448 GGGCCC GGGCC^C
AviII 214 TGCGCA TGC^GCA
BanII 448 GGGCCC G[GA]GC[CT]^C
BciVI 349 GGATAC GTATCC(6/5)
BmyI 448 GGGCCC G[AGT]GC[ACT]^C
BpmI 506 CTCCAG CTGGAG(16/14)
BsaJI 87 CCATGG C^C[ACGT][ACGT]GG
BseRI 407 CTCCTC GAGGAG(10/8)
Bsp1286I 448 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 327 TGTACA T^GTACA
BspLU11I 571 ACATGT A^CATGT
BspMI 419 GCAGGT ACCTGC(4/8)
BsrGI 327 TGTACA T^GTACA
BstAPI 206 GCATAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 230 GGTCACC G^GT[ACGT]ACC
BstPI 230 GGTCACC G^GT[ACGT]ACC
BstYI 612 AGATCC [GA]^GATC[CT]
CfoI 215 GCGC GCG^C
DpnII 613 GATC ^GATC
DraII 448 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 87 CCATGG C^C[GA][CT]GG
EcoO109I 448 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 230 GGTCACC G^GT[ACGT]ACC
EcoRI 92 GAATTC G^AATTC
EcoT14I 87 CCATGG C^C[AT][AT]GG
FspI 214 TGCGCA TGC^GCA
HaeIII 449 GGCC GG^CC
HhaI 215 GCGC GCG^C
HinP1I 215 GCGC G^CGC
HindIII 682 AAGCTT A^AGCTT
MaeII 211 ACGT A^CGT
MboI 613 GATC ^GATC
MflI 612 AGATCC [GA]^GATC[CT]
MseI 661 TTAA T^TAA
MwoI 206 GCATAACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 87 CCATGG C^CATGG
NdeII 613 GATC ^GATC
NspI 571 ACATGT [GA]CATG^[CT]
PleI 201 GACTC GAGTC(4/5)
PshAI 584 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 434 CTGCAG CTGCA^G
Sau3AI 613 GATC ^GATC
SmlI 550 CTTGAG C^T[CT][GA]AG
SspBI 327 TGTACA T^GTACA
StyI 87 CCATGG C^C[AT][AT]GG
TaqI 759 TCGA T^CGA
TfiI 292 GAATC G^A[AT]TC
Tru9I 661 TTAA T^TAA
TthHB8I 759 TCGA T^CGA
XhoII 612 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 92 GAATTC [GA]^AATT[CT]
712 GAATTT
AfaI 328 GTAC GT^AC
525 GTAC
ApoI 92 GAATTC [GA]^AATT[CT]
712 GAATTT
AvaII 401 GGTCC G^G[AT]CC
422 GGTCC
BsiYI 41 CCTCTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
413 CCAAATGCAGG
BslI 41 CCTCTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
413 CCAAATGCAGG
BsrDI 58 GCAATG GCAATG(2/0)
763 GCAATG
BssKI 9 CCTGG ^CC[ACGT]GG
508 CCAGG
BstF5I 736 CATCC GGATG(2/0)
226 GGATG
BstNI 9 CCTGG CC^[AT]GG
508 CCAGG
DdeI 250 CTCAG C^T[ACGT]AG
609 CTTAG
EcoRII 9 CCTGG ^CC[AT]GG
508 CCAGG
EcoRV 36 GATATC GAT^ATC
731 GATATC
FokI 736 CATCC GGATG(9/13)
226 GGATG
HinfI 201 GACTC G^A[ACGT]TC
292 GAATC
MslI 239 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
599 CACCAAAATG
MvaI 9 CCTGG CC^[AT]GG
508 CCAGG
RsaI 328 GTAC GT^AC
525 GTAC
ScrFI 9 CCTGG CC^[ACGT]GG
508 CCAGG
SfcI 137 CTACAG C^T[GA][CT]AG
434 CTGCAG
Tsp45I 231 GTCAC ^GT[GC]AC
701 GTGAC
TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^
529 CAGTG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BsmAI BsrI Cfr13I HphI MaeIII
MboII MnlI NlaIII NlaIV Sau96I
Tsp509I