Restriction map for
pFN21AE4325
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT14I
EcoT22I FbaI FseI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 736 GCGG CCGC(-3/-1)
AclI 939 AACGTT AA^CGTT
AcsI 678 GAATTC [GA]^AATT[CT]
AhdI 962 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 678 GAATTC [GA]^AATT[CT]
AseI 135 ATTAAT AT^TAAT
AsnI 135 ATTAAT AT^TAAT
Asp700I 597 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 962 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 27 TGCGCA TGC^GCA
BanI 205 GGCACC G^G[CT][GA]CC
BanII 853 GAGCCC G[GA]GC[CT]^C
BglII 413 AGATCT A^GATCT
BpmI 520 CTCCAG CTGGAG(16/14)
BsaBI 408 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 88 TCCGGT [AT]^CCGG[AT]
BseRI 77 CTCCTC GAGGAG(10/8)
BstEII 696 GGTGACC G^GT[ACGT]ACC
BstPI 696 GGTGACC G^GT[ACGT]ACC
BstXI 637 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 28 GCGC GCG^C
DraI 498 TTTAAA TTT^AAA
DraII 17 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 962 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 171 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 17 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 696 GGTGACC G^GT[ACGT]ACC
EcoRI 678 GAATTC G^AATTC
FauI 736 GCGGG CCCGC(4/6)
FspI 27 TGCGCA TGC^GCA
HgaI 147 GACGC GACGC(5/10)
HhaI 28 GCGC GCG^C
HinP1I 28 GCGC G^CGC
MaeII 940 ACGT A^CGT
MamI 408 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 433 GAAGA GAAGA(8/7)
PleI 743 GACTC GAGTC(4/5)
PshBI 135 ATTAAT AT^TAAT
Psp1406I 939 AACGTT AA^CGTT
PstI 175 CTGCAG CTGCA^G
PvuII 311 CAGCTG CAG^CTG
ScaI 599 AGTACT AGT^ACT
SexAI 643 ACCTGGT A^CC[AT]GGT
SmaI 105 CCCGGG CCC^GGG
SmlI 587 CTCAAG C^T[CT][GA]AG
StuI 911 AGGCCT AGG^CCT
XmaCI 105 CCCGGG C^CCGGG
XmaI 105 CCCGGG C^CCGGG
XmnI 597 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 443 GTAC GT^AC
600 GTAC
AluI 312 AGCT AG^CT
529 AGCT
AlwNI 235 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
364 CAGTTCCTG
AvaI 68 CCCGAG C^[CT]CG[GA]G
105 CCCGGG
BsoBI 68 CCCGAG C^[CT]CG[GA]G
105 CCCGGG
BstF5I 776 GGATG GGATG(2/0)
801 GGATG
Cac8I 177 GCAGGC GC[ACGT]^[ACGT]GC
313 GCTGGC
DdeI 362 CTCAG C^T[ACGT]AG
702 CTCAG
FokI 776 GGATG GGATG(9/13)
801 GGATG
MspA1I 311 CAGCTG C[AC]G^C[GT]G
734 CAGCGG
MwoI 19 GCCCCTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
66 GCCCCGAGAGC
NlaIII 303 CATG CATG^
929 CATG
PshAI 147 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
225 GACCAATGTC
RsaI 443 GTAC GT^AC
600 GTAC
SfaNI 25 GATGC GCATC(5/9)
922 GATGC
SfcI 175 CTGCAG C^T[GA][CT]AG
574 CTACAG
TfiI 274 GATTC G^A[AT]TC
843 GATTC
Tsp509I 282 AATT ^AATT
679 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AlwI AvaII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI BstYI Cfr13I
DpnII EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MflI MnlI MseI MspI
MvaI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI Tru9I TseI Tsp45I
TspRI XhoII