Restriction map for
pFN21AE4426
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI AscI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglII BlpI BpmI Bpu1102I
BsaHI BsaI BsgI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MflI MluI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 1134 TCCGGA T^CCGGA
AfaI 347 GTAC GT^AC
Alw44I 931 GTGCAC G^TGCAC
AlwI 1108 GATCC GGATC(4/5)
AlwNI 1094 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 815 GGGCCC GGGCC^C
ApaLI 931 GTGCAC G^TGCAC
AseI 909 ATTAAT AT^TAAT
AsnI 909 ATTAAT AT^TAAT
AvrII 936 CCTAGG C^CTAGG
BalI 584 TGGCCA TGG^CCA
BanI 574 GGTGCC G^G[CT][GA]CC
BanII 815 GGGCCC G[GA]GC[CT]^C
BbsI 96 GAAGAC GAAGAC(2/6)
BcnI 350 CCCGG CC^[GC]GG
BglI 577 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 936 CCTAGG C^CTAGG
BpuAI 96 GAAGAC GAAGAC(2/6)
BsaAI 232 TACGTG [CT]AC^GT[GA]
BsaBI 354 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1134 TCCGGA [AT]^CCGG[AT]
BseAI 1134 TCCGGA T^CCGGA
BseRI 868 GAGGAG GAGGAG(10/8)
BsmFI 1096 GTCCC GGGAC(10/14)
BsmI 791 GAATGC GAATGC(1/-1)
BspEI 1134 TCCGGA T^CCGGA
BspHI 521 TCATGA T^CATGA
BspMI 927 GCAGGT ACCTGC(4/8)
BstXI 880 CCATCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 49 GCGC GCG^C
DdeI 1266 CTCAG C^T[ACGT]AG
DsaI 567 CCATGG C^C[GA][CT]GG
EaeI 584 TGGCCA [CT]^GGCC[GA]
EcoT22I 244 ATGCAT ATGCA^T
FauI 267 GCGGG CCCGC(4/6)
HhaI 49 GCGC GCG^C
HinP1I 49 GCGC G^CGC
HphI 1125 GGTGA GGTGA(8/7)
MamI 354 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1195 CAATTG C^AATTG
MluNI 584 TGGCCA TGG^CCA
MroI 1134 TCCGGA T^CCGGA
MscI 584 TGGCCA TGG^CCA
MspA1I 1268 CAGCTG C[AC]G^C[GT]G
MunI 1195 CAATTG C^AATTG
NciI 350 CCCGG CC^[GC]GG
NcoI 567 CCATGG C^CATGG
NsiI 244 ATGCAT ATGCA^T
PleI 1305 GAGTC GAGTC(4/5)
PshAI 543 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 909 ATTAAT AT^TAAT
PvuII 1268 CAGCTG CAG^CTG
RcaI 521 TCATGA T^CATGA
RsaI 347 GTAC GT^AC
SmlI 495 CTCAAG C^T[CT][GA]AG
TaqI 459 TCGA T^CGA
Tsp45I 124 GTCAC ^GT[GC]AC
TthHB8I 459 TCGA T^CGA
XcmI 983 CCAATGTAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 462 AAATTT [GA]^AATT[CT]
1251 AAATTT
ApoI 462 AAATTT [GA]^AATT[CT]
1251 AAATTT
AspHI 931 GTGCAC G[AT]GC[AT]^C
1157 GTGCTC
BclI 338 TGATCA T^GATCA
960 TGATCA
BsiHKAI 931 GTGCAC G[AT]GC[AT]^C
1157 GTGCTC
BsmBI 541 GAGACG CGTCTC(1/5)
716 GAGACG
BssSI 580 CTCGTG CACGAG(-5/-1)
863 CTCGTG
BstF5I 881 CATCC GGATG(2/0)
80 GGATG
Cfr13I 815 GGGCC G^G[ACGT]CC
816 GGCCC
FbaI 338 TGATCA T^GATCA
960 TGATCA
FokI 881 CATCC GGATG(9/13)
80 GGATG
HaeIII 585 GGCC GG^CC
816 GGCC
HapII 351 CCGG C^CGG
1135 CCGG
HindIII 639 AAGCTT A^AGCTT
874 AAGCTT
HpaII 351 CCGG C^CGG
1135 CCGG
MspI 351 CCGG C^CGG
1135 CCGG
NlaIV 574 GGTGCC GG[ACGT]^[ACGT]CC
815 GGGCCC
Sau96I 815 GGGCC G^G[ACGT]CC
816 GGCCC
SfaNI 472 GATGC GCATC(5/9)
422 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbvI BciVI BfaI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstNI DpnII
EcoRII EcoT14I Fnu4HI HinfI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI