Restriction map for
pFN21AE2211
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# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MboII MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 209 CGCG CG^CG
AcsI 99 GAATTC [GA]^AATT[CT]
ApoI 99 GAATTC [GA]^AATT[CT]
AspHI 11 GTGCTC G[AT]GC[AT]^C
AvaI 269 CCCGGG C^[CT]CG[GA]G
AvaII 30 GGTCC G^G[AT]CC
BanII 138 GAGCCC G[GA]GC[CT]^C
BbvCI 143 CCTCAGC CCTCAGC(-5/-2)
BglI 197 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 251 AGATCT A^GATCT
BpmI 259 CTGGAG CTGGAG(16/14)
BsiHKAI 11 GTGCTC G[AT]GC[AT]^C
BsoBI 269 CCCGGG C^[CT]CG[GA]G
BspMI 123 ACCTGC ACCTGC(4/8)
BstUI 209 CGCG CG^CG
BstYI 251 AGATCT [GA]^GATC[CT]
CfoI 210 GCGC GCG^C
DpnII 252 GATC ^GATC
DsaI 163 CCGTGG C^C[GA][CT]GG
EcoRI 99 GAATTC G^AATTC
HapII 270 CCGG C^CGG
HhaI 210 GCGC GCG^C
HinP1I 210 GCGC G^CGC
HinfI 47 GAGTC G^A[ACGT]TC
HpaII 270 CCGG C^CGG
MaeIII 64 GTGAC ^GT[ACGT]AC
MboI 252 GATC ^GATC
MflI 251 AGATCT [GA]^GATC[CT]
MslI 158 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 146 CAGCTG C[AC]G^C[GT]G
MspI 270 CCGG C^CGG
MvnI 209 CGCG CG^CG
NdeII 252 GATC ^GATC
NlaIII 158 CATG CATG^
PleI 47 GAGTC GAGTC(4/5)
PstI 183 CTGCAG CTGCA^G
PvuII 146 CAGCTG CAG^CTG
Sau3AI 252 GATC ^GATC
SexAI 4 ACCAGGT A^CC[AT]GGT
SfaNI 311 GCATC GCATC(5/9)
SfcI 183 CTGCAG C^T[GA][CT]AG
SmaI 269 CCCGGG CCC^GGG
Tsp45I 64 GTGAC ^GT[GC]AC
Tsp509I 100 AATT ^AATT
XhoII 251 AGATCT [GA]^GATC[CT]
XmaCI 269 CCCGGG C^CCGGG
XmaI 269 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AluI 86 AGCT AG^CT
147 AGCT
BcnI 269 CCCGG CC^[GC]GG
270 CCGGG
BmyI 11 GTGCTC G[AGT]GC[ACT]^C
138 GAGCCC
BsmFI 58 GTCCC GGGAC(10/14)
232 GTCCC
Bsp1286I 11 GTGCTC G[AGT]GC[ACT]^C
138 GAGCCC
BsrI 258 ACTGG ACTGG(1/-1)
61 CCAGT
BstF5I 312 CATCC GGATG(2/0)
228 GGATG
EaeI 160 TGGCCG [CT]^GGCC[GA]
327 CGGCCA
FauI 194 CCCGC CCCGC(4/6)
308 CCCGC
FokI 312 CATCC GGATG(9/13)
228 GGATG
NciI 269 CCCGG CC^[GC]GG
270 CCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I DdeI
EcoRII Fnu4HI HaeIII ItaI MnlI
MvaI MwoI Sau96I ScrFI TseI
TspRI