Restriction map for
pFN21AE5718
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AvrII BbeI
BbrPI BbsI BbvCI BcgI BclI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspEI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I Cfr10I CpoI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI HaeII Hin1I
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 2310 GTCTAC GT^[AC][GT]AC
AccII 80 CGCG CG^CG
AflII 1551 CTTAAG C^TTAAG
AhdI 6 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 6 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2317 GAGCTC G[AT]GC[AT]^C
AviII 932 TGCGCA TGC^GCA
BciVI 963 GTATCC GTATCC(6/5)
BcnI 1911 CCGGG CC^[GC]GG
BfrI 1551 CTTAAG C^TTAAG
BglI 2035 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2770 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2770 GCTCAGC GC^T[ACGT]AGC
BsaWI 2071 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2317 GAGCTC G[AT]GC[AT]^C
BsmBI 1679 GAGACG CGTCTC(1/5)
BspDI 937 ATCGAT AT^CGAT
BspHI 84 TCATGA T^CATGA
BspMI 65 ACCTGC ACCTGC(4/8)
BsrBI 609 CCGCTC CCGCTC(-3/-3)
BsrDI 117 GCAATG GCAATG(2/0)
BstBI 881 TTCGAA TT^CGAA
BstUI 80 CGCG CG^CG
BstXI 1028 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2770 GCTCAGC GC^T[ACGT]AGC
ClaI 937 ATCGAT AT^CGAT
DraI 336 TTTAAA TTT^AAA
DraII 2873 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1279 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 792 GACATTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 66 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2873 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1800 ATGCAT ATGCA^T
FauI 777 GCGGG CCCGC(4/6)
FspI 932 TGCGCA TGC^GCA
HindIII 524 AAGCTT A^AGCTT
MspA1I 188 CAGCTG C[AC]G^C[GT]G
MvnI 80 CGCG CG^CG
NciI 1911 CCGGG CC^[GC]GG
NsiI 1800 ATGCAT ATGCA^T
NspV 881 TTCGAA TT^CGAA
PpuMI 2873 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 188 CAGCTG CAG^CTG
RcaI 84 TCATGA T^CATGA
SacI 2317 GAGCTC GAGCT^C
SfcI 740 CTACAG C^T[GA][CT]AG
SfiI 2034 GGCCACGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 881 TTCGAA TT^CGAA
XbaI 2859 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 1768 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1790 CAGTGGCTG
BalI 1811 TGGCCA TGG^CCA
2033 TGGCCA
BamHI 2074 GGATCC G^GATCC
2521 GGATCC
BanI 1287 GGCACC G^G[CT][GA]CC
1690 GGTGCC
BpmI 910 CTCCAG CTGGAG(16/14)
1615 CTGGAG
BsgI 22 GTGCAG GTGCAG(16/14)
1382 GTGCAG
BssSI 349 CACGAG CACGAG(-5/-1)
1783 CACGAG
EaeI 1811 TGGCCA [CT]^GGCC[GA]
2033 TGGCCA
HapII 1911 CCGG C^CGG
2072 CCGG
HgaI 473 GACGC GACGC(5/10)
81 GCGTC
HincII 499 GTCAAC GT[CT]^[GA]AC
951 GTCAAC
HindII 499 GTCAAC GT[CT]^[GA]AC
951 GTCAAC
HpaII 1911 CCGG C^CGG
2072 CCGG
MfeI 227 CAATTG C^AATTG
1634 CAATTG
MluNI 1811 TGGCCA TGG^CCA
2033 TGGCCA
MscI 1811 TGGCCA TGG^CCA
2033 TGGCCA
MslI 619 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1029 CATCCTGGTG
MspI 1911 CCGG C^CGG
2072 CCGG
MunI 227 CAATTG C^AATTG
1634 CAATTG
NspI 1361 ACATGC [GA]CATG^[CT]
2330 GCATGT
PflMI 1814 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2428 CCAAGGTTTGG
SapI 1596 GAAGAGC GCTCTTC(1/4)
2319 GCTCTTC
StuI 1859 AGGCCT AGG^CCT
2624 AGGCCT
Van91I 1814 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2428 CCAAGGTTTGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BbvI
BfaI BglII BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI