Restriction map for
pFN21AE4422
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstXI
BstYI Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeII MamI MflI MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 148 GTATAC GT^[AC][GT]AC
AccII 81 CGCG CG^CG
AciI 80 CCGC CCGC(-3/-1)
AlwI 247 GATCC GGATC(4/5)
Asp700I 859 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 50 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 524 TGGCCA TGG^CCA
BanI 630 GGTGCC G^G[CT][GA]CC
BanII 75 GAGCCC G[GA]GC[CT]^C
BbsI 460 GAAGAC GAAGAC(2/6)
BciVI 1305 GTATCC GTATCC(6/5)
BcnI 11 CCCGG CC^[GC]GG
BfaI 613 CTAG C^TAG
BpuAI 460 GAAGAC GAAGAC(2/6)
BsaI 29 GAGACC GGTCTC(1/5)
BsgI 680 GTGCAG GTGCAG(16/14)
BsiEI 138 CGATCG CG[GA][CT]^CG
Bsp1407I 110 TGTACA T^GTACA
BspMI 160 GCAGGT ACCTGC(4/8)
BsrGI 110 TGTACA T^GTACA
Bst1107I 148 GTATAC GTA^TAC
BstAPI 1251 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 277 GGTTACC G^GT[ACGT]ACC
BstPI 277 GGTTACC G^GT[ACGT]ACC
BstUI 81 CGCG CG^CG
BstZ17I 148 GTATAC GTA^TAC
DdeI 1271 CTCAG C^T[ACGT]AG
DraII 346 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1171 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 524 TGGCCA [CT]^GGCC[GA]
EcoO109I 346 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 277 GGTTACC G^GT[ACGT]ACC
EcoRI 487 GAATTC G^AATTC
FauI 79 CCCGC CCCGC(4/6)
HaeII 581 AGCGCC [GA]GCGC^[CT]
HapII 12 CCGG C^CGG
HincII 1152 GTCAAC GT[CT]^[GA]AC
HindII 1152 GTCAAC GT[CT]^[GA]AC
HpaII 12 CCGG C^CGG
MaeI 613 CTAG C^TAG
MluNI 524 TGGCCA TGG^CCA
MscI 524 TGGCCA TGG^CCA
MspA1I 356 CAGCTG C[AC]G^C[GT]G
MspI 12 CCGG C^CGG
MvnI 81 CGCG CG^CG
NciI 11 CCCGG CC^[GC]GG
PflFI 50 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 223 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 346 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 455 CTGCAG CTGCA^G
PvuI 138 CGATCG CGAT^CG
PvuII 356 CAGCTG CAG^CTG
SfaNI 1239 GATGC GCATC(5/9)
SspBI 110 TGTACA T^GTACA
SspI 194 AATATT AAT^ATT
Tsp45I 1177 GTGAC ^GT[GC]AC
Tth111I 50 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 223 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1170 CCACCCTGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 859 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 769 ACATGT A^C[GA][CT]GT
1118 ACATGT
AseI 442 ATTAAT AT^TAAT
643 ATTAAT
AsnI 442 ATTAAT AT^TAAT
643 ATTAAT
BmyI 75 GAGCCC G[AGT]GC[ACT]^C
967 GTGCCC
Bsp1286I 75 GAGCCC G[AGT]GC[ACT]^C
967 GTGCCC
BspLU11I 769 ACATGT A^CATGT
1118 ACATGT
BstNI 229 CCTGG CC^[AT]GG
292 CCTGG
DpnII 139 GATC ^GATC
247 GATC
EcoNI 398 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
651 CCTGAAGGAGG
EcoRII 229 CCTGG ^CC[AT]GG
292 CCTGG
EcoT14I 125 CCAAGG C^C[AT][AT]GG
1103 CCAAGG
MaeIII 278 GTTAC ^GT[ACGT]AC
1177 GTGAC
MboI 139 GATC ^GATC
247 GATC
MfeI 4 CAATTG C^AATTG
733 CAATTG
MslI 1078 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1224 CACAAACATG
MunI 4 CAATTG C^AATTG
733 CAATTG
MvaI 229 CCTGG CC^[AT]GG
292 CCTGG
NdeII 139 GATC ^GATC
247 GATC
PleI 1269 GACTC GAGTC(4/5)
335 GAGTC
PshBI 442 ATTAAT AT^TAAT
643 ATTAAT
Sau3AI 139 GATC ^GATC
247 GATC
SfcI 455 CTGCAG C^T[GA][CT]AG
1292 CTACAG
StyI 125 CCAAGG C^C[AT][AT]GG
1103 CCAAGG
TaqI 303 TCGA T^CGA
1113 TCGA
TthHB8I 303 TCGA T^CGA
1113 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I CfoI
Cfr13I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MboII MnlI
MseI MwoI NlaIII NlaIV NspI
RsaI Sau96I ScrFI TfiI Tru9I
TseI Tsp509I TspRI