Restriction map for
pFN21AE5301
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 352 AAATTC [GA]^AATT[CT]
AfaI 648 GTAC GT^AC
Alw44I 258 GTGCAC G^TGCAC
AlwI 687 GATCC GGATC(4/5)
ApaI 590 GGGCCC GGGCC^C
ApaLI 258 GTGCAC G^TGCAC
ApoI 352 AAATTC [GA]^AATT[CT]
AvaI 97 CCCGAG C^[CT]CG[GA]G
BbeI 145 GGCGCC GGCGC^C
BpmI 565 CTGGAG CTGGAG(16/14)
BseRI 28 GAGGAG GAGGAG(10/8)
BsoBI 97 CCCGAG C^[CT]CG[GA]G
DpnII 687 GATC ^GATC
DraI 349 TTTAAA TTT^AAA
EaeI 134 TGGCCG [CT]^GGCC[GA]
EcoNI 21 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 458 CCTTGG C^C[AT][AT]GG
FseI 678 GGCCGGCC GGCCGG^CC
HgaI 93 GACGC GACGC(5/10)
KasI 145 GGCGCC G^GCGCC
MaeII 284 ACGT A^CGT
MboI 687 GATC ^GATC
MseI 350 TTAA T^TAA
MslI 477 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 145 GGCGCC GG^CGCC
NdeII 687 GATC ^GATC
PleI 605 GAGTC GAGTC(4/5)
PpuMI 745 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 598 CTGCAG CTGCA^G
PvuII 419 CAGCTG CAG^CTG
RsaI 648 GTAC GT^AC
SacI 190 GAGCTC GAGCT^C
Sau3AI 687 GATC ^GATC
ScaI 647 AGTACT AGT^ACT
SfaNI 380 GCATC GCATC(5/9)
SfcI 598 CTGCAG C^T[GA][CT]AG
SfiI 535 GGCCTCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 145 GGCGCC GGC^GCC
StuI 543 AGGCCT AGG^CCT
StyI 458 CCTTGG C^C[AT][AT]GG
TfiI 411 GAATC G^A[AT]TC
Tru9I 350 TTAA T^TAA
Tsp509I 353 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 93 GACGCC G[GA]^CG[CT]C
145 GGCGCC
BanII 190 GAGCTC G[GA]GC[CT]^C
590 GGGCCC
BsaHI 93 GACGCC G[GA]^CG[CT]C
145 GGCGCC
BsgI 339 CTGCAC GTGCAG(16/14)
497 GTGCAG
BsmI 372 GAATGC GAATGC(1/-1)
579 GCATTC
BspMI 304 ACCTGC ACCTGC(4/8)
80 GCAGGT
DdeI 487 CTCAG C^T[ACGT]AG
540 CTCAG
Hin1I 93 GACGCC G[GA]^CG[CT]C
145 GGCGCC
HinfI 411 GAATC G^A[ACGT]TC
605 GAGTC
KspI 50 CCGCGG CCGC^GG
314 CCGCGG
MboII 17 TCTTC GAAGA(8/7)
356 TCTTC
NlaIII 453 CATG CATG^
480 CATG
SacII 50 CCGCGG CCGC^GG
314 CCGCGG
TspRI 434 CAGTG CA[GC]TG[ACGT][ACGT]^
725 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BbvI BcnI BglI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIV Sau96I ScrFI
TseI