Restriction map for
pFN21AE4561
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BcgI BcnI BglII
BlnI BsaHI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI BstBI BstEII BstPI ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MluI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 385 GTATAC GT^[AC][GT]AC
AccII 859 CGCG CG^CG
AccIII 461 TCCGGA T^CCGGA
AcsI 1223 AAATTC [GA]^AATT[CT]
AflII 2047 CTTAAG C^TTAAG
AocI 273 CCTGAGG CC^T[ACGT]AGG
ApoI 1223 AAATTC [GA]^AATT[CT]
AvaI 105 CTCGGG C^[CT]CG[GA]G
BanI 1174 GGCACC G^G[CT][GA]CC
BbrPI 1183 CACGTG CAC^GTG
BbsI 280 GAAGAC GAAGAC(2/6)
BciVI 585 GGATAC GTATCC(6/5)
BclI 422 TGATCA T^GATCA
BfrI 2047 CTTAAG C^TTAAG
BglI 1735 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 280 GAAGAC GAAGAC(2/6)
BsaAI 1183 CACGTG [CT]AC^GT[GA]
BseAI 461 TCCGGA T^CCGGA
BsmI 1928 GCATTC GAATGC(1/-1)
BsoBI 105 CTCGGG C^[CT]CG[GA]G
BspEI 461 TCCGGA T^CCGGA
BspHI 1415 TCATGA T^CATGA
BspMI 966 GCAGGT ACCTGC(4/8)
BsrBI 928 GAGCGG CCGCTC(-3/-3)
BsrDI 1265 CATTGC GCAATG(2/0)
BsrFI 1670 ACCGGC [GA]^CCGG[CT]
Bst1107I 385 GTATAC GTA^TAC
BstUI 859 CGCG CG^CG
BstZ17I 385 GTATAC GTA^TAC
Bsu36I 273 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1670 ACCGGC [GA]^CCGG[CT]
DraIII 1811 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 273 CCTGAGG CC^T[ACGT]AGG
FbaI 422 TGATCA T^GATCA
HgaI 11 GCGTC GACGC(5/10)
HincII 1324 GTCAAC GT[CT]^[GA]AC
HindII 1324 GTCAAC GT[CT]^[GA]AC
MfeI 390 CAATTG C^AATTG
MroI 461 TCCGGA T^CCGGA
MunI 390 CAATTG C^AATTG
MvnI 859 CGCG CG^CG
NdeI 428 CATATG CA^TATG
PmaCI 1183 CACGTG CAC^GTG
PmlI 1183 CACGTG CAC^GTG
PpuMI 353 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 547 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1415 TCATGA T^CATGA
SapI 1196 GCTCTTC GCTCTTC(1/4)
SexAI 1812 ACCTGGT A^CC[AT]GGT
SspI 1079 AATATT AAT^ATT
StuI 977 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1302 GATCC GGATC(4/5)
1531 GGATC
BanII 1797 GAGCCC G[GA]GC[CT]^C
1901 GAGCCC
BfaI 1003 CTAG C^TAG
1478 CTAG
BsaBI 1210 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1296 GATCGAGATC
BsaI 454 GAGACC GGTCTC(1/5)
1697 GGTCTC
BsaWI 461 TCCGGA [AT]^CCGG[AT]
548 ACCGGA
BstAPI 561 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
765 GCAGAGTCTGC
BstXI 1373 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1935 CCACCTGGCTGG
BstYI 1301 AGATCC [GA]^GATC[CT]
1531 GGATCT
DraII 353 AGGACCT [GA]G^G[ACGT]CC[CT]
1034 AGGCCCT
EarI 1197 CTCTTC CTCTTC(1/4)
1155 GAAGAG
EcoNI 48 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
357 CCTGTGGAAGG
EcoO109I 353 AGGACCT [GA]G^G[ACGT]CC[CT]
1034 AGGCCCT
FauI 909 GCGGG CCCGC(4/6)
1269 GCGGG
Ksp632I 1197 CTCTTC CTCTTC(1/4)
1155 GAAGAG
MaeI 1003 CTAG C^TAG
1478 CTAG
MaeII 1184 ACGT A^CGT
1427 ACGT
MamI 1210 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1296 GATCGAGATC
MflI 1301 AGATCC [GA]^GATC[CT]
1531 GGATCT
MseI 2036 TTAA T^TAA
2048 TTAA
NcoI 324 CCATGG C^CATGG
1761 CCATGG
SfaNI 232 GATGC GCATC(5/9)
1337 GCATC
Tru9I 2036 TTAA T^TAA
2048 TTAA
XcmI 236 CCAATGGTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
488 CCATGCCAAGGCTGG
XhoII 1301 AGATCC [GA]^GATC[CT]
1531 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BalI BbvCI BbvI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I