Restriction map for
pFN21AE4533
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BsaAI BsiEI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HgaI
KpnI MfeI MluI MluNI MscI
MunI NaeI NgoMIV NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1405 GACGTC GACGT^C
AccIII 1204 TCCGGA T^CCGGA
AlwNI 94 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1765 CCTCAGG CC^T[ACGT]AGG
ApaI 1153 GGGCCC GGGCC^C
AspI 663 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 587 GGATCC G^GATCC
BbeI 1315 GGCGCC GGCGC^C
BbsI 857 GAAGAC GAAGAC(2/6)
BciVI 1207 GGATAC GTATCC(6/5)
BlpI 414 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 414 GCTAAGC GC^T[ACGT]AGC
BpuAI 857 GAAGAC GAAGAC(2/6)
BseAI 1204 TCCGGA T^CCGGA
BseRI 266 CTCCTC GAGGAG(10/8)
Bsp1407I 1731 TGTACA T^GTACA
BspEI 1204 TCCGGA T^CCGGA
BspMI 272 ACCTGC ACCTGC(4/8)
BsrGI 1731 TGTACA T^GTACA
BssSI 1634 CTCGTG CACGAG(-5/-1)
BstBI 254 TTCGAA TT^CGAA
Bsu36I 1765 CCTCAGG CC^T[ACGT]AGG
CelII 414 GCTAAGC GC^T[ACGT]AGC
EaeI 1560 TGGCCG [CT]^GGCC[GA]
Eco81I 1765 CCTCAGG CC^T[ACGT]AGG
EcoNI 1390 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 679 GATATC GAT^ATC
EcoT14I 404 CCATGG C^C[AT][AT]GG
HaeII 1315 GGCGCC [GA]GCGC^[CT]
HindIII 7 AAGCTT A^AGCTT
HpaI 1063 GTTAAC GTT^AAC
KasI 1315 GGCGCC G^GCGCC
KspI 1158 CCGCGG CCGC^GG
MroI 1204 TCCGGA T^CCGGA
MslI 969 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1315 GGCGCC GG^CGCC
NcoI 404 CCATGG C^CATGG
NdeI 374 CATATG CA^TATG
NheI 1696 GCTAGC G^CTAGC
NspI 757 GCATGC [GA]CATG^[CT]
NspV 254 TTCGAA TT^CGAA
PflFI 663 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1305 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1772 GAGTC GAGTC(4/5)
PpuMI 95 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 1158 CCGCGG CCGC^GG
SfoI 1315 GGCGCC GGC^GCC
SfuI 254 TTCGAA TT^CGAA
SphI 757 GCATGC GCATG^C
SspBI 1731 TGTACA T^GTACA
StyI 404 CCATGG C^C[AT][AT]GG
Tth111I 663 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1305 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1230 CCATCACCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 154 CGCG CG^CG
1159 CGCG
AcsI 245 AAATTC [GA]^AATT[CT]
882 AAATTT
AcyI 1315 GGCGCC G[GA]^CG[CT]C
1405 GACGTC
AfaI 1224 GTAC GT^AC
1732 GTAC
ApoI 245 AAATTC [GA]^AATT[CT]
882 AAATTT
Asp700I 814 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1287 GAATCTCTTC
AspHI 477 GAGCTC G[AT]GC[AT]^C
1163 GAGCTC
BanI 613 GGTGCC G^G[CT][GA]CC
1315 GGCGCC
BbvCI 493 CCTCAGC CCTCAGC(-5/-2)
1098 CCTCAGC
BfaI 808 CTAG C^TAG
1697 CTAG
BpmI 1215 CTCCAG CTGGAG(16/14)
701 CTGGAG
BsaBI 918 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1419 GATGGTCATC
BsaHI 1315 GGCGCC G[GA]^CG[CT]C
1405 GACGTC
BsaI 163 GAGACC GGTCTC(1/5)
799 GAGACC
BsaWI 1204 TCCGGA [AT]^CCGG[AT]
1440 ACCGGA
BsgI 1706 CTGCAC GTGCAG(16/14)
1616 GTGCAG
BsiHKAI 477 GAGCTC G[AT]GC[AT]^C
1163 GAGCTC
BstUI 154 CGCG CG^CG
1159 CGCG
BstXI 537 CCACGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
603 CCAGTTTGTTGG
BstYI 587 GGATCC [GA]^GATC[CT]
1469 GGATCT
DsaI 404 CCATGG C^C[GA][CT]GG
1158 CCGCGG
Hin1I 1315 GGCGCC G[GA]^CG[CT]C
1405 GACGTC
MaeI 808 CTAG C^TAG
1697 CTAG
MaeII 368 ACGT A^CGT
1406 ACGT
MamI 918 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1419 GATGGTCATC
MflI 587 GGATCC [GA]^GATC[CT]
1469 GGATCT
MvnI 154 CGCG CG^CG
1159 CGCG
PvuII 1351 CAGCTG CAG^CTG
1556 CAGCTG
RsaI 1224 GTAC GT^AC
1732 GTAC
SacI 477 GAGCTC GAGCT^C
1163 GAGCTC
SmaI 524 CCCGGG CCC^GGG
671 CCCGGG
StuI 992 AGGCCT AGG^CCT
1037 AGGCCT
TaqI 161 TCGA T^CGA
255 TCGA
TthHB8I 161 TCGA T^CGA
255 TCGA
XhoII 587 GGATCC [GA]^GATC[CT]
1469 GGATCT
XmaCI 524 CCCGGG C^CCGGG
671 CCCGGG
XmaI 524 CCCGGG C^CCGGG
671 CCCGGG
XmnI 814 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1287 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI