Restriction map for
pFN21AE5502
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FauI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MaeII MamI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Alw44I 152 GTGCAC G^TGCAC
AlwI 556 GGATC GGATC(4/5)
ApaLI 152 GTGCAC G^TGCAC
AspHI 152 GTGCAC G[AT]GC[AT]^C
AvaI 740 CCCGAG C^[CT]CG[GA]G
BclI 1155 TGATCA T^GATCA
BglII 507 AGATCT A^GATCT
BpmI 326 CTGGAG CTGGAG(16/14)
BsaJI 1082 CCTTGG C^C[ACGT][ACGT]GG
BsiEI 62 CGGTCG CG[GA][CT]^CG
BsiHKAI 152 GTGCAC G[AT]GC[AT]^C
BsoBI 740 CCCGAG C^[CT]CG[GA]G
BspHI 1164 TCATGA T^CATGA
BsrI 157 CCAGT ACTGG(1/-1)
BssKI 853 CCAGG ^CC[ACGT]GG
BssSI 1049 CACGAG CACGAG(-5/-1)
BstNI 853 CCAGG CC^[AT]GG
BstYI 507 AGATCT [GA]^GATC[CT]
Cac8I 465 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 125 GGGCC G^G[ACGT]CC
DdeI 478 CTTAG C^T[ACGT]AG
DraII 124 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 851 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 55 GACATGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 124 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 853 CCAGG ^CC[AT]GG
EcoT14I 1082 CCTTGG C^C[AT][AT]GG
EcoT22I 535 ATGCAT ATGCA^T
FbaI 1155 TGATCA T^GATCA
MflI 507 AGATCT [GA]^GATC[CT]
MslI 1159 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 853 CCAGG CC^[AT]GG
NdeI 348 CATATG CA^TATG
NlaIV 717 GGAACC GG[ACGT]^[ACGT]CC
NsiI 535 ATGCAT ATGCA^T
PstI 364 CTGCAG CTGCA^G
RcaI 1164 TCATGA T^CATGA
Sau96I 125 GGGCC G^G[ACGT]CC
ScrFI 853 CCAGG CC^[ACGT]GG
SexAI 852 ACCAGGT A^CC[AT]GGT
SfaNI 382 GATGC GCATC(5/9)
SfcI 364 CTGCAG C^T[GA][CT]AG
StuI 1079 AGGCCT AGG^CCT
StyI 1082 CCTTGG C^C[AT][AT]GG
XbaI 510 TCTAGA T^CTAGA
XhoII 507 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 698 AAATTT [GA]^AATT[CT]
1023 AAATTT
ApoI 698 AAATTT [GA]^AATT[CT]
1023 AAATTT
BalI 297 TGGCCA TGG^CCA
389 TGGCCA
BmyI 152 GTGCAC G[AGT]GC[ACT]^C
420 GGGCAC
BsaI 448 GAGACC GGTCTC(1/5)
817 GGTCTC
BseRI 269 CTCCTC GAGGAG(10/8)
972 GAGGAG
BsmAI 448 GAGAC GTCTC(1/5)
818 GTCTC
Bsp1286I 152 GTGCAC G[AGT]GC[ACT]^C
420 GGGCAC
EaeI 297 TGGCCA [CT]^GGCC[GA]
389 TGGCCA
EarI 565 GAAGAG CTCTTC(1/4)
969 GAAGAG
Ksp632I 565 GAAGAG CTCTTC(1/4)
969 GAAGAG
MfeI 88 CAATTG C^AATTG
773 CAATTG
MluNI 297 TGGCCA TGG^CCA
389 TGGCCA
MscI 297 TGGCCA TGG^CCA
389 TGGCCA
MunI 88 CAATTG C^AATTG
773 CAATTG
NspI 148 GCATGT [GA]CATG^[CT]
1113 ACATGC
PleI 43 GACTC GAGTC(4/5)
1099 GAGTC
SmlI 491 CTCAAG C^T[CT][GA]AG
677 CTCAAG
Tsp45I 1067 GTGAC ^GT[GC]AC
1101 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BfaI BsiYI
BslI DpnII Fnu4HI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspA1I MwoI NdeII
NlaIII Sau3AI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I