Restriction map for
pFN21AE4476
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AspEI AspI
AvaI AviII AvrII BalI BbrPI
BbvCI BcgI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV FauI FseI FspI HgaI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI PvuII RcaI
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 846 GGTACC G^GTACC
AcyI 73 GGCGCC G[GA]^CG[CT]C
AflIII 168 ACGTGT A^C[GA][CT]GT
AocI 957 CCTAAGG CC^T[ACGT]AGG
ApaI 1318 GGGCCC GGGCC^C
AseI 1396 ATTAAT AT^TAAT
AsnI 1396 ATTAAT AT^TAAT
Asp700I 790 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 846 GGTACC G^GTACC
BamHI 425 GGATCC G^GATCC
BbeI 73 GGCGCC GGCGC^C
BciVI 1268 GTATCC GTATCC(6/5)
BclI 1464 TGATCA T^GATCA
BglI 76 GCCCCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 167 TACGTG [CT]AC^GT[GA]
BsaHI 73 GGCGCC G[GA]^CG[CT]C
BsaWI 9 TCCGGT [AT]^CCGG[AT]
BseRI 40 GAGGAG GAGGAG(10/8)
BsmFI 625 GGGAC GGGAC(10/14)
BsmI 255 GCATTC GAATGC(1/-1)
BspMI 1122 ACCTGC ACCTGC(4/8)
BsrBI 92 CCGCTC CCGCTC(-3/-3)
Bsu36I 957 CCTAAGG CC^T[ACGT]AGG
CfoI 74 GCGC GCG^C
CpoI 50 CGGACCG CG^G[AT]CCG
DraI 855 TTTAAA TTT^AAA
EaeI 1172 TGGCCG [CT]^GGCC[GA]
Eco81I 957 CCTAAGG CC^T[ACGT]AGG
EcoNI 1000 CCTTATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1464 TGATCA T^GATCA
HaeII 73 GGCGCC [GA]GCGC^[CT]
HhaI 74 GCGC GCG^C
Hin1I 73 GGCGCC G[GA]^CG[CT]C
HinP1I 74 GCGC G^CGC
KasI 73 GGCGCC G^GCGCC
KpnI 846 GGTACC GGTAC^C
MaeII 168 ACGT A^CGT
NarI 73 GGCGCC GG^CGCC
NcoI 1470 CCATGG C^CATGG
NdeI 605 CATATG CA^TATG
PshAI 678 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1396 ATTAAT AT^TAAT
RsrII 50 CGGACCG CG^G[AT]CCG
SapI 1217 GCTCTTC GCTCTTC(1/4)
SfoI 73 GGCGCC GGC^GCC
SmlI 320 CTCAAG C^T[CT][GA]AG
SphI 546 GCATGC GCATG^C
SspI 711 AATATT AAT^ATT
TaqI 15 TCGA T^CGA
Tsp45I 746 GTGAC ^GT[GC]AC
TthHB8I 15 TCGA T^CGA
XmnI 790 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 54 CCGC CCGC(-3/-1)
92 CCGC
BbsI 558 GTCTTC GAAGAC(2/6)
685 GTCTTC
BcnI 26 CCCGG CC^[GC]GG
70 CCGGG
BpmI 95 CTCCAG CTGGAG(16/14)
374 CTGGAG
BpuAI 558 GTCTTC GAAGAC(2/6)
685 GTCTTC
BsaI 17 GAGACC GGTCTC(1/5)
5 GGTCTC
BsiYI 643 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1000 CCTTATTTAGG
BslI 643 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1000 CCTTATTTAGG
BsmAI 17 GAGAC GTCTC(1/5)
6 GTCTC
BstF5I 309 GGATG GGATG(2/0)
325 GGATG
EcoT22I 161 ATGCAT ATGCA^T
544 ATGCAT
FokI 309 GGATG GGATG(9/13)
325 GGATG
NciI 26 CCCGG CC^[GC]GG
70 CCGGG
NsiI 161 ATGCAT ATGCA^T
544 ATGCAT
NspI 244 GCATGT [GA]CATG^[CT]
546 GCATGC
PstI 467 CTGCAG CTGCA^G
1124 CTGCAG
SfcI 467 CTGCAG C^T[GA][CT]AG
1124 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BanI BanII BbvI
BfaI BmyI BsaJI BsiHKAI Bsp1286I
BsrDI BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII