Restriction map for
pFN21AE3626
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 255 GTAC GT^AC
AlwI 510 GATCC GGATC(4/5)
ApaI 562 GGGCCC GGGCC^C
BciVI 180 GGATAC GTATCC(6/5)
BcnI 311 CCCGG CC^[GC]GG
BpmI 239 CTGGAG CTGGAG(16/14)
BseRI 64 CTCCTC GAGGAG(10/8)
BsrDI 461 GCAATG GCAATG(2/0)
BsrI 596 CCAGT ACTGG(1/-1)
BssKI 311 CCCGG ^CC[ACGT]GG
BssSI 617 CTCGTG CACGAG(-5/-1)
BstYI 509 AGATCC [GA]^GATC[CT]
Cac8I 457 GCCTGC GC[ACGT]^[ACGT]GC
DpnII 510 GATC ^GATC
FauI 374 CCCGC CCCGC(4/6)
HapII 312 CCGG C^CGG
HincII 142 GTCAAC GT[CT]^[GA]AC
HindII 142 GTCAAC GT[CT]^[GA]AC
HpaII 312 CCGG C^CGG
HphI 212 TCACC GGTGA(8/7)
MboI 510 GATC ^GATC
MflI 509 AGATCC [GA]^GATC[CT]
MseI 482 TTAA T^TAA
MslI 42 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 312 CCGG C^CGG
NciI 311 CCCGG CC^[GC]GG
NdeII 510 GATC ^GATC
PleI 370 GAGTC GAGTC(4/5)
PpuMI 591 GGGACCC [GA]G^G[AT]CC[CT]
PstI 70 CTGCAG CTGCA^G
PvuII 236 CAGCTG CAG^CTG
RsaI 255 GTAC GT^AC
SacI 131 GAGCTC GAGCT^C
Sau3AI 510 GATC ^GATC
ScrFI 311 CCCGG CC^[ACGT]GG
TfiI 196 GAATC G^A[AT]TC
Tru9I 482 TTAA T^TAA
Tsp509I 576 AATT ^AATT
XhoII 509 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AhdI 364 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
622 GACATAGGGTC
AluI 132 AGCT AG^CT
237 AGCT
AspEI 364 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
622 GACATAGGGTC
AspHI 131 GAGCTC G[AT]GC[AT]^C
242 GAGCAC
AvaII 592 GGACC G^G[AT]CC
629 GGTCC
BanII 131 GAGCTC G[GA]GC[CT]^C
562 GGGCCC
BsiHKAI 131 GAGCTC G[AT]GC[AT]^C
242 GAGCAC
BsmAI 38 GAGAC GTCTC(1/5)
62 GTCTC
BsmFI 591 GGGAC GGGAC(10/14)
372 GTCCC
BstF5I 306 GGATG GGATG(2/0)
381 GGATG
DraII 562 GGGCCCC [GA]G^G[ACGT]CC[CT]
591 GGGACCC
Eam1105I 364 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
622 GACATAGGGTC
EcoNI 221 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
407 CCTTTGCTAGG
EcoO109I 562 GGGCCCC [GA]G^G[ACGT]CC[CT]
591 GGGACCC
FokI 306 GGATG GGATG(9/13)
381 GGATG
HinfI 196 GAATC G^A[ACGT]TC
370 GAGTC
MaeIII 362 GTGAC ^GT[ACGT]AC
620 GTGAC
MspA1I 236 CAGCTG C[AC]G^C[GT]G
607 CAGCGG
SfaNI 307 GATGC GCATC(5/9)
551 GCATC
SfcI 70 CTGCAG C^T[GA][CT]AG
225 CTACAG
StuI 404 AGGCCT AGG^CCT
473 AGGCCT
Tsp45I 362 GTGAC ^GT[GC]AC
620 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI BbvI BfaI BmyI BsiYI
BslI Bsp1286I Cfr13I EarI Fnu4HI
HaeIII ItaI Ksp632I MaeI MboII
MnlI MwoI NlaIII NlaIV Sau96I
TseI TspRI