##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       BstUI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       ItaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        TseI        Tsp45I      Tth111I     Van91I      
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI          249  GTATAC               GT^[AC][GT]AC
AfaI           76  GTAC                 GT^AC
AflII          66  CTTAAG               C^TTAAG
Asp700I       528  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          833  CTCGAG               C^[CT]CG[GA]G
BamHI         194  GGATCC               G^GATCC
BbsI          790  GAAGAC               GAAGAC(2/6)
BfrI           66  CTTAAG               C^TTAAG
BmyI          205  GTGCCC               G[AGT]GC[ACT]^C
BpuAI         790  GAAGAC               GAAGAC(2/6)
BsaAI          77  TACGTG               [CT]AC^GT[GA]
BsmFI         755  GGGAC                GGGAC(10/14)
BsoBI         833  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      205  GTGCCC               G[AGT]GC[ACT]^C
BspHI         356  TCATGA               T^CATGA
Bst1107I      249  GTATAC               GTA^TAC
BstZ17I       249  GTATAC               GTA^TAC
Cfr13I        780  GGCCC                G^G[ACGT]CC
DraIII        257  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          847  CCATGG               C^C[GA][CT]GG
MaeIII        540  GTTAC                ^GT[ACGT]AC
MslI          496  CACATTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          847  CCATGG               C^CATGG
NlaIV         194  GGATCC               GG[ACGT]^[ACGT]CC
PaeR7I        833  CTCGAG               C^TCGAG
PleI          273  GAGTC                GAGTC(4/5)
RcaI          356  TCATGA               T^CATGA
RsaI           76  GTAC                 GT^AC
Sau96I        780  GGCCC                G^G[ACGT]CC
TaqI          834  TCGA                 T^CGA
TfiI          156  GATTC                G^A[AT]TC
TthHB8I       834  TCGA                 T^CGA
XcmI          368  CCAGAGATGTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          833  CTCGAG               C^TCGAG
XmnI          528  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BglII         590  AGATCT               A^GATCT
              825  AGATCT              
BssKI         227  CCTGG                ^CC[ACGT]GG
              603  CCTGG               
BstNI         227  CCTGG                CC^[AT]GG
              603  CCTGG               
BstXI         427  CCAAAGTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              495  CCACATTCATGG        
EcoRII        227  CCTGG                ^CC[AT]GG
              603  CCTGG               
HaeIII        339  GGCC                 GG^CC
              780  GGCC                
HinfI         156  GATTC                G^A[ACGT]TC
              273  GAGTC               
HphI          345  GGTGA                GGTGA(8/7)
              223  TCACC               
MvaI          227  CCTGG                CC^[AT]GG
              603  CCTGG               
ScrFI         227  CCTGG                CC^[ACGT]GG
              603  CCTGG               
SspI          114  AATATT               AAT^ATT
              443  AATATT              
TspRI         261  CAGTG                CA[GC]TG[ACGT][ACGT]^
              776  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        AlwI        BsaJI       BseRI       BsiYI       
BslI        BsrI        BstF5I      BstYI       DdeI        
DpnII       EcoT14I     FokI        MaeII       MboI        
MboII       MflI        MnlI        MseI        NdeII       
NlaIII      Sau3AI      SfaNI       SmlI        StyI        
Tru9I       Tsp509I     XhoII