Restriction map for
pFN21AE6218
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaJI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MluI MroI MslI MspA1I
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 64 GTATAC GT^[AC][GT]AC
Asp700I 774 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 33 GAGCAC G[AT]GC[AT]^C
BalI 149 TGGCCA TGG^CCA
BamHI 745 GGATCC G^GATCC
BbsI 778 GTCTTC GAAGAC(2/6)
BbvI 712 GCTGC GCAGC(8/12)
BcnI 590 CCCGG CC^[GC]GG
BglII 785 AGATCT A^GATCT
BpuAI 778 GTCTTC GAAGAC(2/6)
BsaI 593 GGTCTC GGTCTC(1/5)
BsaWI 29 ACCGGA [AT]^CCGG[AT]
BseRI 121 CTCCTC GAGGAG(10/8)
BsiHKAI 33 GAGCAC G[AT]GC[AT]^C
BspMI 658 ACCTGC ACCTGC(4/8)
BsrBI 503 GAGCGG CCGCTC(-3/-3)
Bst1107I 64 GTATAC GTA^TAC
BstZ17I 64 GTATAC GTA^TAC
CfoI 797 GCGC GCG^C
DraII 608 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 149 TGGCCA [CT]^GGCC[GA]
EarI 445 CTCTTC CTCTTC(1/4)
EcoNI 816 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 608 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 250 GAATTC G^AATTC
Fnu4HI 712 GCTGC GC^[ACGT]GC
HgaI 791 GCGTC GACGC(5/10)
HhaI 797 GCGC GCG^C
HinP1I 797 GCGC G^CGC
HindIII 398 AAGCTT A^AGCTT
ItaI 712 GCTGC GC^[ACGT]GC
Ksp632I 445 CTCTTC CTCTTC(1/4)
MaeIII 328 GTTAC ^GT[ACGT]AC
MfeI 188 CAATTG C^AATTG
MluNI 149 TGGCCA TGG^CCA
MscI 149 TGGCCA TGG^CCA
MunI 188 CAATTG C^AATTG
NciI 590 CCCGG CC^[GC]GG
SapI 444 GCTCTTC GCTCTTC(1/4)
SbfI 659 CCTGCAGG CCTGCA^GG
SexAI 291 ACCAGGT A^CC[AT]GGT
Sse8387I 659 CCTGCAGG CCTGCA^GG
TaqI 782 TCGA T^CGA
TseI 712 GCTGC G^C[AT]GC
TthHB8I 782 TCGA T^CGA
XmnI 774 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 90 GCGG CCGC(-3/-1)
505 GCGG
AcsI 250 GAATTC [GA]^AATT[CT]
412 AAATTT
AfaI 18 GTAC GT^AC
306 GTAC
AlwNI 635 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
827 CAGGAGCTG
AocI 7 CCTCAGG CC^T[ACGT]AGG
207 CCTGAGG
ApoI 250 GAATTC [GA]^AATT[CT]
412 AAATTT
AvaII 382 GGTCC G^G[AT]CC
836 GGTCC
BmyI 33 GAGCAC G[AGT]GC[ACT]^C
510 GGGCAC
BsmFI 285 GTCCC GGGAC(10/14)
582 GTCCC
Bsp1286I 33 GAGCAC G[AGT]GC[ACT]^C
510 GGGCAC
BspHI 125 TCATGA T^CATGA
176 TCATGA
BstNI 43 CCTGG CC^[AT]GG
292 CCAGG
BstYI 745 GGATCC [GA]^GATC[CT]
785 AGATCT
Bsu36I 7 CCTCAGG CC^T[ACGT]AGG
207 CCTGAGG
Cac8I 86 GCTGGC GC[ACGT]^[ACGT]GC
640 GCTGGC
Eco81I 7 CCTCAGG CC^T[ACGT]AGG
207 CCTGAGG
EcoRII 43 CCTGG ^CC[AT]GG
292 CCAGG
HapII 30 CCGG C^CGG
591 CCGG
HpaII 30 CCGG C^CGG
591 CCGG
HphI 141 GGTGA GGTGA(8/7)
24 TCACC
MboII 446 TCTTC GAAGA(8/7)
779 TCTTC
MflI 745 GGATCC [GA]^GATC[CT]
785 AGATCT
MseI 133 TTAA T^TAA
701 TTAA
MspI 30 CCGG C^CGG
591 CCGG
MvaI 43 CCTGG CC^[AT]GG
292 CCAGG
PleI 244 GACTC GAGTC(4/5)
580 GAGTC
PstI 452 CTGCAG CTGCA^G
660 CTGCAG
RcaI 125 TCATGA T^CATGA
176 TCATGA
RsaI 18 GTAC GT^AC
306 GTAC
TfiI 165 GATTC G^A[AT]TC
682 GAATC
Tru9I 133 TTAA T^TAA
701 TTAA
TspRI 332 CAGTG CA[GC]TG[ACGT][ACGT]^
515 CAGTG
XhoII 745 GGATCC [GA]^GATC[CT]
785 AGATCT
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BfaI BsiYI BslI
BsmAI BsrI BssKI Cfr13I DdeI
DpnII HaeIII HinfI MaeI MboI
MnlI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
Tsp509I