Restriction map for
pFN21AE1447
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII AccI AccII AclI AcyI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspHI AspI AvaII AviII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeIII MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 487 GGTACC G^GTACC
AccIII 56 TCCGGA T^CCGGA
AciI 179 GCGG CCGC(-3/-1)
AflII 637 CTTAAG C^TTAAG
AhdI 655 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 559 GGATC GGATC(4/5)
Asp700I 465 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 487 GGTACC G^GTACC
AspEI 655 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 322 CTCGAG C^[CT]CG[GA]G
AvrII 630 CCTAGG C^CTAGG
BanI 487 GGTACC G^G[CT][GA]CC
BbvI 117 GCTGC GCAGC(8/12)
BfaI 631 CTAG C^TAG
BfrI 637 CTTAAG C^TTAAG
BglII 792 AGATCT A^GATCT
BlnI 630 CCTAGG C^CTAGG
BsaI 710 GAGACC GGTCTC(1/5)
BsaWI 56 TCCGGA [AT]^CCGG[AT]
BseAI 56 TCCGGA T^CCGGA
BseRI 15 GAGGAG GAGGAG(10/8)
BsgI 185 GTGCAG GTGCAG(16/14)
BsoBI 322 CTCGAG C^[CT]CG[GA]G
BspEI 56 TCCGGA T^CCGGA
BstYI 792 AGATCT [GA]^GATC[CT]
Cac8I 92 GCATGC GC[ACGT]^[ACGT]GC
Eam1105I 655 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 100 GAATTC G^AATTC
Fnu4HI 117 GCTGC GC^[ACGT]GC
HapII 57 CCGG C^CGG
HpaII 57 CCGG C^CGG
ItaI 117 GCTGC GC^[ACGT]GC
KpnI 487 GGTACC GGTAC^C
MaeI 631 CTAG C^TAG
MaeII 329 ACGT A^CGT
MflI 792 AGATCT [GA]^GATC[CT]
MroI 56 TCCGGA T^CCGGA
MslI 612 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 57 CCGG C^CGG
NcoI 668 CCATGG C^CATGG
NdeI 418 CATATG CA^TATG
NlaIV 487 GGTACC GG[ACGT]^[ACGT]CC
NspI 92 GCATGC [GA]CATG^[CT]
PaeR7I 322 CTCGAG C^TCGAG
SphI 92 GCATGC GCATG^C
TseI 117 GCTGC G^C[AT]GC
XhoI 322 CTCGAG C^TCGAG
XhoII 792 AGATCT [GA]^GATC[CT]
XmnI 465 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 488 GTAC GT^AC
555 GTAC
AlwNI 528 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
616 CAGATGCTG
BsiYI 72 CCATTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
714 CCTGCTTTGGG
BslI 72 CCATTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
714 CCTGCTTTGGG
BsmFI 407 GGGAC GGGAC(10/14)
536 GGGAC
BsrI 87 CCAGT ACTGG(1/-1)
588 CCAGT
BstF5I 450 CATCC GGATG(2/0)
576 GGATG
EcoT14I 630 CCTAGG C^C[AT][AT]GG
668 CCATGG
FokI 450 CATCC GGATG(9/13)
576 GGATG
HphI 190 GGTGA GGTGA(8/7)
697 GGTGA
RsaI 488 GTAC GT^AC
555 GTAC
SmlI 322 CTCGAG C^T[CT][GA]AG
637 CTTAAG
StyI 630 CCTAGG C^C[AT][AT]GG
668 CCATGG
TaqI 316 TCGA T^CGA
323 TCGA
TspRI 88 CAGTG CA[GC]TG[ACGT][ACGT]^
528 CAGTG
TthHB8I 316 TCGA T^CGA
323 TCGA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BsmAI
BspMI BssKI BstNI DdeI DpnII
DsaI EcoRII HinfI MboI MboII
MnlI MseI MvaI NdeII NlaIII
Sau3AI ScrFI SfaNI TfiI Tru9I
Tsp509I