Restriction map for
pFN21AE5603
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbvCI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI MwoI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 294 GTCTAC GT^[AC][GT]AC
AfaI 1359 GTAC GT^AC
AflIII 777 ACATGT A^C[GA][CT]GT
Alw44I 859 GTGCAC G^TGCAC
ApaLI 859 GTGCAC G^TGCAC
Asp700I 1090 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 859 GTGCAC G[AT]GC[AT]^C
AvaI 1228 CCCGAG C^[CT]CG[GA]G
BalI 114 TGGCCA TGG^CCA
BanII 972 GAGCCC G[GA]GC[CT]^C
BbrPI 1253 CACGTG CAC^GTG
BcgI 1007 CGAGGACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 867 TGATCA T^GATCA
BsaI 1154 GAGACC GGTCTC(1/5)
BseRI 384 GAGGAG GAGGAG(10/8)
BsiHKAI 859 GTGCAC G[AT]GC[AT]^C
BsoBI 1228 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1358 TGTACA T^GTACA
BspLU11I 777 ACATGT A^CATGT
BsrGI 1358 TGTACA T^GTACA
BssSI 314 CACGAG CACGAG(-5/-1)
BstEII 375 GGTTACC G^GT[ACGT]ACC
BstPI 375 GGTTACC G^GT[ACGT]ACC
BstXI 342 CCAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 708 GCTGGC GC[ACGT]^[ACGT]GC
DraI 717 TTTAAA TTT^AAA
DraIII 131 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 924 CCATGG C^C[GA][CT]GG
EaeI 114 TGGCCA [CT]^GGCC[GA]
EcoO65I 375 GGTTACC G^GT[ACGT]ACC
FbaI 867 TGATCA T^GATCA
HindIII 1410 AAGCTT A^AGCTT
MluNI 114 TGGCCA TGG^CCA
MscI 114 TGGCCA TGG^CCA
NcoI 924 CCATGG C^CATGG
PmaCI 1253 CACGTG CAC^GTG
PmlI 1253 CACGTG CAC^GTG
PstI 19 CTGCAG CTGCA^G
RsaI 1359 GTAC GT^AC
SfaNI 101 GCATC GCATC(5/9)
SnaBI 297 TACGTA TAC^GTA
SspBI 1358 TGTACA T^GTACA
StuI 1379 AGGCCT AGG^CCT
SwaI 716 ATTTAAAT ATTT^AAAT
TfiI 361 GATTC G^A[AT]TC
XmnI 1090 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 424 GAATTT [GA]^AATT[CT]
490 AAATTT
ApoI 424 GAATTT [GA]^AATT[CT]
490 AAATTT
BbsI 691 GAAGAC GAAGAC(2/6)
160 GTCTTC
BmyI 859 GTGCAC G[AGT]GC[ACT]^C
972 GAGCCC
BpuAI 691 GAAGAC GAAGAC(2/6)
160 GTCTTC
BsaAI 297 TACGTA [CT]AC^GT[GA]
1253 CACGTG
BsmI 999 GCATTC GAATGC(1/-1)
1050 GCATTC
Bsp1286I 859 GTGCAC G[AGT]GC[ACT]^C
972 GAGCCC
DraII 726 GGGACCC [GA]G^G[ACGT]CC[CT]
1009 AGGACCT
EarI 827 GAAGAG CTCTTC(1/4)
961 GAAGAG
EcoO109I 726 GGGACCC [GA]G^G[ACGT]CC[CT]
1009 AGGACCT
HaeII 15 GGCGCT [GA]GCGC^[CT]
33 GGCGCT
HgaI 199 GACGC GACGC(5/10)
671 GACGC
Ksp632I 827 GAAGAG CTCTTC(1/4)
961 GAAGAG
MaeII 298 ACGT A^CGT
1254 ACGT
MseI 718 TTAA T^TAA
1069 TTAA
MslI 102 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1267 CATTTGGATG
NspI 674 GCATGT [GA]CATG^[CT]
777 ACATGT
PpuMI 726 GGGACCC [GA]G^G[AT]CC[CT]
1009 AGGACCT
SfcI 19 CTGCAG C^T[GA][CT]AG
1383 CTGTAG
Tru9I 718 TTAA T^TAA
1069 TTAA
Tsp45I 576 GTCAC ^GT[GC]AC
1223 GTGAC
XcmI 146 CCACTTTTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
698 CCAGGCGGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BfaI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstYI CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MspI MvaI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XhoII