Restriction map for
pFN21AE1511
##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MflI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AciI 293 GCGG CCGC(-3/-1)
AlwNI 725 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 35 GAGCTC G[AT]GC[AT]^C
AvaI 920 CTCGGG C^[CT]CG[GA]G
BalI 327 TGGCCA TGG^CCA
BanII 35 GAGCTC G[GA]GC[CT]^C
BbsI 849 GAAGAC GAAGAC(2/6)
BbvI 234 GCAGC GCAGC(8/12)
BcgI 734 GCACACCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 35 GAGCTC G[AGT]GC[ACT]^C
BpuAI 849 GAAGAC GAAGAC(2/6)
BseRI 62 GAGGAG GAGGAG(10/8)
BsiHKAI 35 GAGCTC G[AT]GC[AT]^C
BsmBI 539 GAGACG CGTCTC(1/5)
BsoBI 920 CTCGGG C^[CT]CG[GA]G
Bsp1286I 35 GAGCTC G[AGT]GC[ACT]^C
BspMI 155 ACCTGC ACCTGC(4/8)
BssSI 59 CACGAG CACGAG(-5/-1)
CfoI 5 GCGC GCG^C
DpnII 349 GATC ^GATC
EaeI 327 TGGCCA [CT]^GGCC[GA]
EcoNI 441 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 382 GATATC GAT^ATC
Fnu4HI 234 GCAGC GC^[ACGT]GC
HaeII 4 AGCGCC [GA]GCGC^[CT]
HhaI 5 GCGC GCG^C
HinP1I 5 GCGC G^CGC
HincII 697 GTTGAC GT[CT]^[GA]AC
HindII 697 GTTGAC GT[CT]^[GA]AC
ItaI 234 GCAGC GC^[ACGT]GC
MaeII 542 ACGT A^CGT
MboI 349 GATC ^GATC
MfeI 311 CAATTG C^AATTG
MluNI 327 TGGCCA TGG^CCA
MscI 327 TGGCCA TGG^CCA
MslI 360 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 235 CAGCTG C[AC]G^C[GT]G
MunI 311 CAATTG C^AATTG
MwoI 320 GCATCTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 349 GATC ^GATC
PpuMI 152 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 235 CAGCTG CAG^CTG
SacI 35 GAGCTC GAGCT^C
Sau3AI 349 GATC ^GATC
SfcI 83 CTACAG C^T[GA][CT]AG
TaqI 870 TCGA T^CGA
TseI 234 GCAGC G^C[AT]GC
Tsp45I 493 GTGAC ^GT[GC]AC
TthHB8I 870 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsaI 296 GAGACC GGTCTC(1/5)
769 GGTCTC
DraII 78 AGGCCCT [GA]G^G[ACGT]CC[CT]
152 GGGACCT
EcoO109I 78 AGGCCCT [GA]G^G[ACGT]CC[CT]
152 GGGACCT
HphI 492 GGTGA GGTGA(8/7)
630 GGTGA
MaeIII 493 GTGAC ^GT[ACGT]AC
865 GTAAC
NlaIII 331 CATG CATG^
421 CATG
PflMI 300 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
724 CCAGCTACTGG
PleI 495 GACTC GAGTC(4/5)
356 GAGTC
SfaNI 370 GATGC GCATC(5/9)
320 GCATC
SmlI 109 CTTGAG C^T[CT][GA]AG
534 CTTGAG
Van91I 300 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
724 CCAGCTACTGG
XcmI 342 CCACCCTGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
478 CCAGGATTCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI AvaII BfaI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII EcoT14I FokI HaeIII HinfI
MaeI MboII MnlI MseI MvaI
NlaIV Sau96I ScrFI StyI TfiI
Tru9I Tsp509I TspRI