Restriction map for
pFN21AE6225
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI BstAPI BstEII
BstPI BstUI BstXI CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
TspRI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 620 CCGC CCGC(-3/-1)
AcsI 97 AAATTT [GA]^AATT[CT]
AocI 340 CCTGAGG CC^T[ACGT]AGG
ApoI 97 AAATTT [GA]^AATT[CT]
BclI 368 TGATCA T^GATCA
BcnI 462 CCGGG CC^[GC]GG
BlpI 456 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
BsaWI 56 TCCGGT [AT]^CCGG[AT]
BspMI 569 ACCTGC ACCTGC(4/8)
BsrDI 222 CATTGC GCAATG(2/0)
Bst1107I 282 GTATAC GTA^TAC
BstBI 406 TTCGAA TT^CGAA
BstF5I 54 CATCC GGATG(2/0)
BstYI 106 GGATCT [GA]^GATC[CT]
BstZ17I 282 GTATAC GTA^TAC
Bsu36I 340 CCTGAGG CC^T[ACGT]AGG
Cac8I 226 GCCAGC GC[ACGT]^[ACGT]GC
CelII 456 GCTGAGC GC^T[ACGT]AGC
Cfr13I 546 GGCCC G^G[ACGT]CC
DraII 545 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 340 CCTGAGG CC^T[ACGT]AGG
EcoO109I 545 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 619 CCCGC CCCGC(4/6)
FbaI 368 TGATCA T^GATCA
FokI 54 CATCC GGATG(9/13)
HaeIII 546 GGCC GG^CC
HincII 335 GTCGAC GT[CT]^[GA]AC
HindII 335 GTCGAC GT[CT]^[GA]AC
HphI 579 GGTGA GGTGA(8/7)
MaeIII 417 GTTAC ^GT[ACGT]AC
MflI 106 GGATCT [GA]^GATC[CT]
MspA1I 74 CAGCTG C[AC]G^C[GT]G
MwoI 355 GCCAAGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 462 CCGGG CC^[GC]GG
NcoI 426 CCATGG C^CATGG
NlaIII 427 CATG CATG^
NlaIV 48 GGAGCC GG[ACGT]^[ACGT]CC
NspV 406 TTCGAA TT^CGAA
PflMI 53 CCATCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 511 GACTC GAGTC(4/5)
PvuII 74 CAGCTG CAG^CTG
SalI 335 GTCGAC G^TCGAC
SapI 502 GAAGAGC GCTCTTC(1/4)
Sau96I 546 GGCCC G^G[ACGT]CC
SfuI 406 TTCGAA TT^CGAA
TfiI 560 GAATC G^A[AT]TC
Van91I 53 CCATCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 106 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 282 GTATAC GT^[AC][GT]AC
335 GTCGAC
AfaI 163 GTAC GT^AC
196 GTAC
AlwI 315 GATCC GGATC(4/5)
106 GGATC
BanII 49 GAGCCC G[GA]GC[CT]^C
266 GAGCCC
BmyI 49 GAGCCC G[AGT]GC[ACT]^C
266 GAGCCC
BpmI 513 CTCCAG CTGGAG(16/14)
77 CTGGAG
BsiYI 53 CCATCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
570 CCTGCCCACGG
BslI 53 CCATCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
570 CCTGCCCACGG
Bsp1286I 49 GAGCCC G[AGT]GC[ACT]^C
266 GAGCCC
BstNI 477 CCTGG CC^[AT]GG
598 CCTGG
DdeI 341 CTGAG C^T[ACGT]AG
457 CTGAG
DsaI 426 CCATGG C^C[GA][CT]GG
575 CCACGG
EarI 91 GAAGAG CTCTTC(1/4)
502 GAAGAG
EcoRII 477 CCTGG ^CC[AT]GG
598 CCTGG
EcoT14I 426 CCATGG C^C[AT][AT]GG
607 CCAAGG
HapII 57 CCGG C^CGG
462 CCGG
HindIII 16 AAGCTT A^AGCTT
632 AAGCTT
HinfI 511 GACTC G^A[ACGT]TC
560 GAATC
HpaII 57 CCGG C^CGG
462 CCGG
Ksp632I 91 GAAGAG CTCTTC(1/4)
502 GAAGAG
MseI 130 TTAA T^TAA
188 TTAA
MspI 57 CCGG C^CGG
462 CCGG
MvaI 477 CCTGG CC^[AT]GG
598 CCTGG
PstI 71 CTGCAG CTGCA^G
110 CTGCAG
RsaI 163 GTAC GT^AC
196 GTAC
SfaNI 352 GATGC GCATC(5/9)
625 GATGC
StyI 426 CCATGG C^C[AT][AT]GG
607 CCAAGG
TaqI 336 TCGA T^CGA
407 TCGA
Tru9I 130 TTAA T^TAA
188 TTAA
Tsp509I 98 AATT ^AATT
273 AATT
TthHB8I 336 TCGA T^CGA
407 TCGA
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BsaJI BssKI DpnII
Fnu4HI ItaI MboI MboII MnlI
NdeII Sau3AI ScrFI SfcI TseI