Restriction map for
pFN21AE4321
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AfaI AflII AflIII AgeI AhdI
AlwI AlwNI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DpnII DraI
DraII DrdI Eam1105I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MboI MfeI MflI MluI
MseI MunI NaeI NarI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 907 GTCTAC GT^[AC][GT]AC
AccIII 329 TCCGGA T^CCGGA
Alw44I 810 GTGCAC G^TGCAC
AocI 124 CCTCAGG CC^T[ACGT]AGG
Aor51HI 186 AGCGCT AGC^GCT
ApaI 39 GGGCCC GGGCC^C
ApaLI 810 GTGCAC G^TGCAC
Asp700I 507 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 625 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 315 GGACC G^G[AT]CC
BalI 714 TGGCCA TGG^CCA
BbsI 631 GTCTTC GAAGAC(2/6)
BbvCI 588 CCTCAGC CCTCAGC(-5/-2)
BfaI 608 CTAG C^TAG
BglI 41 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 259 CTGGAG CTGGAG(16/14)
BpuAI 631 GTCTTC GAAGAC(2/6)
BsaWI 329 TCCGGA [AT]^CCGG[AT]
BseAI 329 TCCGGA T^CCGGA
BseRI 301 CTCCTC GAGGAG(10/8)
BsiEI 964 CGGCCG CG[GA][CT]^CG
BsmFI 789 GTCCC GGGAC(10/14)
BsmI 234 GCATTC GAATGC(1/-1)
BspEI 329 TCCGGA T^CCGGA
BspMI 354 GCAGGT ACCTGC(4/8)
BsrI 251 CCAGT ACTGG(1/-1)
BstAPI 205 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 124 CCTCAGG CC^T[ACGT]AGG
DraIII 400 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 964 CGGCCG C^GGCCG
EclXI 964 CGGCCG C^GGCCG
Eco47III 186 AGCGCT AGC^GCT
Eco52I 964 CGGCCG C^GGCCG
Eco81I 124 CCTCAGG CC^T[ACGT]AGG
HaeII 186 AGCGCT [GA]GCGC^[CT]
HinfI 945 GAGTC G^A[ACGT]TC
KspI 43 CCGCGG CCGC^GG
MaeI 608 CTAG C^TAG
MaeII 629 ACGT A^CGT
MluNI 714 TGGCCA TGG^CCA
MroI 329 TCCGGA T^CCGGA
MscI 714 TGGCCA TGG^CCA
NcoI 728 CCATGG C^CATGG
NspI 194 GCATGC [GA]CATG^[CT]
PaeR7I 554 CTCGAG C^TCGAG
PflFI 625 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 945 GAGTC GAGTC(4/5)
PvuII 8 CAGCTG CAG^CTG
SacII 43 CCGCGG CCGC^GG
SexAI 401 ACCTGGT A^CC[AT]GGT
SphI 194 GCATGC GCATG^C
Tth111I 625 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 554 CTCGAG C^TCGAG
XmnI 507 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 810 GTGCAC G[AT]GC[AT]^C
841 GAGCAC
BanI 772 GGCACC G^G[CT][GA]CC
897 GGTGCC
BcgI 377 GCATGGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
843 GCACGACCATCG
BsaI 287 GGTCTC GGTCTC(1/5)
688 GGTCTC
BsgI 265 CTGCAC GTGCAG(16/14)
87 GTGCAG
BsiHKAI 810 GTGCAC G[AT]GC[AT]^C
841 GAGCAC
BsmAI 288 GTCTC GTCTC(1/5)
689 GTCTC
BsrDI 575 CATTGC GCAATG(2/0)
723 CATTGC
BssHII 65 GCGCGC G^CGCGC
92 GCGCGC
BstXI 251 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
722 CCATTGCCATGG
EarI 306 CTCTTC CTCTTC(1/4)
942 GAAGAG
HphI 619 GGTGA GGTGA(8/7)
291 TCACC
Ksp632I 306 CTCTTC CTCTTC(1/4)
942 GAAGAG
MaeIII 254 GTGAC ^GT[ACGT]AC
675 GTCAC
MspA1I 8 CAGCTG C[AC]G^C[GT]G
43 CCGCGG
PflMI 372 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
399 CCACCTGGTGG
SmlI 554 CTCGAG C^T[CT][GA]AG
868 CTCAAG
Tsp45I 254 GTGAC ^GT[GC]AC
675 GTCAC
Van91I 372 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
399 CCACCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI SmaI StyI TaqI
TseI TspRI TthHB8I XmaCI XmaI