Restriction map for
pFN21AE2292
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbvCI BclI BfaI
BfrI BglI BglII BlnI BmyI
BpmI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 438 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 365 AGCGCT AGC^GCT
BbrPI 573 CACGTG CAC^GTG
BcgI 378 GCAAATCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 251 GGATAC GTATCC(6/5)
BsaAI 573 CACGTG [CT]AC^GT[GA]
Bsp1407I 577 TGTACA T^GTACA
BspMI 374 ACCTGC ACCTGC(4/8)
BsrGI 577 TGTACA T^GTACA
BsrI 880 CCAGT ACTGG(1/-1)
BstF5I 299 GGATG GGATG(2/0)
BstYI 20 GGATCT [GA]^GATC[CT]
CfoI 366 GCGC GCG^C
DsaI 136 CCACGG C^C[GA][CT]GG
EaeI 46 TGGCCG [CT]^GGCC[GA]
Eco47III 365 AGCGCT AGC^GCT
FokI 299 GGATG GGATG(9/13)
HaeII 365 AGCGCT [GA]GCGC^[CT]
HaeIII 47 GGCC GG^CC
HgaI 307 GACGC GACGC(5/10)
HhaI 366 GCGC GCG^C
HinP1I 366 GCGC G^CGC
HphI 663 TCACC GGTGA(8/7)
MaeII 574 ACGT A^CGT
MfeI 498 CAATTG C^AATTG
MflI 20 GGATCT [GA]^GATC[CT]
MspA1I 56 CAGCTG C[AC]G^C[GT]G
MunI 498 CAATTG C^AATTG
PmaCI 573 CACGTG CAC^GTG
PmlI 573 CACGTG CAC^GTG
PstI 847 CTGCAG CTGCA^G
PvuII 56 CAGCTG CAG^CTG
SapI 874 GAAGAGC GCTCTTC(1/4)
SspBI 577 TGTACA T^GTACA
XhoII 20 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 578 GTAC GT^AC
659 GTAC
AflIII 572 ACACGT A^C[GA][CT]GT
574 ACGTGT
AlwI 346 GATCC GGATC(4/5)
20 GGATC
AocI 260 CCTTAGG CC^T[ACGT]AGG
550 CCTGAGG
AvaII 265 GGTCC G^G[AT]CC
555 GGACC
BcnI 623 CCCGG CC^[GC]GG
671 CCGGG
BlpI 4 GCTCAGC GC^T[ACGT]AGC
678 GCTGAGC
Bpu1102I 4 GCTCAGC GC^T[ACGT]AGC
678 GCTGAGC
BsgI 635 GTGCAG GTGCAG(16/14)
904 GTGCAG
BsmAI 197 GAGAC GTCTC(1/5)
314 GAGAC
BsmFI 958 GGGAC GGGAC(10/14)
506 GTCCC
BsrDI 153 CATTGC GCAATG(2/0)
646 GCAATG
Bsu36I 260 CCTTAGG CC^T[ACGT]AGG
550 CCTGAGG
CelII 4 GCTCAGC GC^T[ACGT]AGC
678 GCTGAGC
Cfr13I 265 GGTCC G^G[ACGT]CC
555 GGACC
EarI 517 GAAGAG CTCTTC(1/4)
874 GAAGAG
Eco81I 260 CCTTAGG CC^T[ACGT]AGG
550 CCTGAGG
EcoT14I 854 CCAAGG C^C[AT][AT]GG
869 CCAAGG
Ksp632I 517 GAAGAG CTCTTC(1/4)
874 GAAGAG
MseI 146 TTAA T^TAA
421 TTAA
MslI 455 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
642 CATCGCAATG
NciI 623 CCCGG CC^[GC]GG
671 CCGGG
NspI 255 ACATGC [GA]CATG^[CT]
460 ACATGC
RsaI 578 GTAC GT^AC
659 GTAC
Sau96I 265 GGTCC G^G[ACGT]CC
555 GGACC
SfaNI 760 GATGC GCATC(5/9)
427 GCATC
SfcI 467 CTGTAG C^T[GA][CT]AG
847 CTGCAG
StyI 854 CCAAGG C^C[AT][AT]GG
869 CCAAGG
TfiI 93 GATTC G^A[AT]TC
830 GAATC
Tru9I 146 TTAA T^TAA
421 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbsI BbvI BpuAI BsaJI
BssKI BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI HapII HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
PleI Sau3AI ScrFI TseI Tsp45I
Tsp509I TspRI