Restriction map for
pFN21AE3638
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AvaI AvrII BalI BamHI
BbrPI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HapII HincII
HindII HindIII HpaI HpaII KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MspI MunI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 890 GGTACC G^GTACC
AccII 37 CGCG CG^CG
AlwI 720 GATCC GGATC(4/5)
ApaI 912 GGGCCC GGGCC^C
Asp718I 890 GGTACC G^GTACC
AspI 1243 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1117 GGCGCC GGCGC^C
BbsI 232 GAAGAC GAAGAC(2/6)
BbvCI 1045 GCTGAGG CCTCAGC(-5/-2)
BglI 10 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 232 GAAGAC GAAGAC(2/6)
BsaI 157 GAGACC GGTCTC(1/5)
BsgI 844 GTGCAG GTGCAG(16/14)
BsmI 579 GAATGC GAATGC(1/-1)
BspHI 812 TCATGA T^CATGA
BsrBI 1015 GAGCGG CCGCTC(-3/-3)
BstUI 37 CGCG CG^CG
BstYI 719 AGATCC [GA]^GATC[CT]
EarI 423 GAAGAG CTCTTC(1/4)
EcoNI 1062 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1117 GGCGCC [GA]GCGC^[CT]
KasI 1117 GGCGCC G^GCGCC
KpnI 890 GGTACC GGTAC^C
Ksp632I 423 GAAGAG CTCTTC(1/4)
MflI 719 AGATCC [GA]^GATC[CT]
MseI 518 TTAA T^TAA
MspA1I 1076 CAGCTG C[AC]G^C[GT]G
MvnI 37 CGCG CG^CG
NarI 1117 GGCGCC GG^CGCC
NspI 773 GCATGC [GA]CATG^[CT]
PflFI 1243 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1058 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 959 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1076 CAGCTG CAG^CTG
RcaI 812 TCATGA T^CATGA
SacI 937 GAGCTC GAGCT^C
SapI 423 GAAGAGC GCTCTTC(1/4)
SbfI 1163 CCTGCAGG CCTGCA^GG
SexAI 71 ACCAGGT A^CC[AT]GGT
SfoI 1117 GGCGCC GGC^GCC
SphI 773 GCATGC GCATG^C
Sse8387I 1163 CCTGCAGG CCTGCA^GG
TaqI 1145 TCGA T^CGA
TfiI 1072 GAATC G^A[AT]TC
Tru9I 518 TTAA T^TAA
Tth111I 1243 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1145 TCGA T^CGA
XcmI 786 CCAAGCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 719 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 46 GGCGTC G[GA]^CG[CT]C
1117 GGCGCC
AlwNI 1040 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1231 CAGAGCCTG
AocI 330 CCTAAGG CC^T[ACGT]AGG
382 CCTAAGG
AviII 176 TGCGCA TGC^GCA
1136 TGCGCA
BbvI 709 GCAGC GCAGC(8/12)
801 GCAGC
BpmI 1228 CTCCAG CTGGAG(16/14)
55 CTGGAG
BsaHI 46 GGCGTC G[GA]^CG[CT]C
1117 GGCGCC
BsmAI 109 GAGAC GTCTC(1/5)
157 GAGAC
BsmFI 272 GGGAC GGGAC(10/14)
133 GTCCC
BstF5I 24 GGATG GGATG(2/0)
1206 GGATG
BstXI 20 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1203 CCAGGATGCTGG
Bsu36I 330 CCTAAGG CC^T[ACGT]AGG
382 CCTAAGG
DpnII 720 GATC ^GATC
1143 GATC
DraII 912 GGGCCCT [GA]G^G[ACGT]CC[CT]
1058 AGGACCT
Eco81I 330 CCTAAGG CC^T[ACGT]AGG
382 CCTAAGG
EcoO109I 912 GGGCCCT [GA]G^G[ACGT]CC[CT]
1058 AGGACCT
FauI 35 CCCGC CCCGC(4/6)
632 CCCGC
FokI 24 GGATG GGATG(9/13)
1206 GGATG
FspI 176 TGCGCA TGC^GCA
1136 TGCGCA
HgaI 47 GCGTC GACGC(5/10)
396 GCGTC
Hin1I 46 GGCGTC G[GA]^CG[CT]C
1117 GGCGCC
HphI 685 GGTGA GGTGA(8/7)
549 TCACC
MboI 720 GATC ^GATC
1143 GATC
NdeII 720 GATC ^GATC
1143 GATC
PstI 746 CTGCAG CTGCA^G
1164 CTGCAG
Sau3AI 720 GATC ^GATC
1143 GATC
TseI 709 GCAGC G^C[AT]GC
801 GCAGC
Tsp45I 548 GTCAC ^GT[GC]AC
1249 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanI BanII BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DsaI
EcoRII EcoT14I Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MaeIII MboII
MnlI MslI MvaI MwoI NcoI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI SfcI SmlI StyI
Tsp509I TspRI