Restriction map for
pFN21AE6712
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BciVI
BfrI BglI BglII BlnI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
Ksp632I KspI MfeI MluI MroI
MunI NdeI NotI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1747 GGTACC G^GTACC
AccI 2689 GTCTAC GT^[AC][GT]AC
AcsI 1594 GAATTT [GA]^AATT[CT]
ApaI 2215 GGGCCC GGGCC^C
ApoI 1594 GAATTT [GA]^AATT[CT]
Asp718I 1747 GGTACC G^GTACC
BamHI 1834 GGATCC G^GATCC
BbeI 982 GGCGCC GGCGC^C
BbrPI 2665 CACGTG CAC^GTG
BbsI 727 GTCTTC GAAGAC(2/6)
BclI 1940 TGATCA T^GATCA
BlpI 519 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 519 GCTGAGC GC^T[ACGT]AGC
BpuAI 727 GTCTTC GAAGAC(2/6)
BsaAI 2665 CACGTG [CT]AC^GT[GA]
BsaBI 957 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 267 CGACCG CG[GA][CT]^CG
BsmBI 1289 GAGACG CGTCTC(1/5)
BsmI 1464 GAATGC GAATGC(1/-1)
Bsp1407I 1070 TGTACA T^GTACA
BsrGI 1070 TGTACA T^GTACA
BstAPI 2661 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1158 GGTGACC G^GT[ACGT]ACC
BstPI 1158 GGTGACC G^GT[ACGT]ACC
CelII 519 GCTGAGC GC^T[ACGT]AGC
DraIII 1762 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1708 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1158 GGTGACC G^GT[ACGT]ACC
FbaI 1940 TGATCA T^GATCA
KasI 982 GGCGCC G^GCGCC
KpnI 1747 GGTACC GGTAC^C
MamI 957 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1603 TTAA T^TAA
NarI 982 GGCGCC GG^CGCC
NcoI 2621 CCATGG C^CATGG
NheI 214 GCTAGC G^CTAGC
NruI 152 TCGCGA TCG^CGA
PflMI 2752 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2665 CACGTG CAC^GTG
PmlI 2665 CACGTG CAC^GTG
PstI 2659 CTGCAG CTGCA^G
ScaI 1885 AGTACT AGT^ACT
SexAI 2325 ACCTGGT A^CC[AT]GGT
SfoI 982 GGCGCC GGC^GCC
SgrAI 864 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1070 TGTACA T^GTACA
Tru9I 1603 TTAA T^TAA
Van91I 2752 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2167 TCTAGA T^CTAGA
XcmI 1232 CCAGTGCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BalI 1758 TGGCCA TGG^CCA
2345 TGGCCA
BbvCI 1272 CCTCAGC CCTCAGC(-5/-2)
1751 CCTCAGC
BcgI 143 GCAACGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1461 GCAGAATGCTCG
BsaWI 247 ACCGGA [AT]^CCGG[AT]
2379 TCCGGT
BseRI 18 GAGGAG GAGGAG(10/8)
283 GAGGAG
BsrBI 463 CCGCTC CCGCTC(-3/-3)
21 GAGCGG
EcoT14I 159 CCTTGG C^C[AT][AT]GG
2621 CCATGG
MluNI 1758 TGGCCA TGG^CCA
2345 TGGCCA
MscI 1758 TGGCCA TGG^CCA
2345 TGGCCA
NaeI 865 GCCGGC GCC^GGC
2614 GCCGGC
NgoMIV 865 GCCGGC G^CCGGC
2614 GCCGGC
PaeR7I 466 CTCGAG C^TCGAG
1742 CTCGAG
PvuII 1754 CAGCTG CAG^CTG
2726 CAGCTG
SmaI 1414 CCCGGG CCC^GGG
2048 CCCGGG
StuI 11 AGGCCT AGG^CCT
2247 AGGCCT
StyI 159 CCTTGG C^C[AT][AT]GG
2621 CCATGG
TfiI 224 GATTC G^A[AT]TC
1912 GATTC
XhoI 466 CTCGAG C^TCGAG
1742 CTCGAG
XmaCI 1414 CCCGGG C^CCGGG
2048 CCCGGG
XmaI 1414 CCCGGG C^CCGGG
2048 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI Asp700I AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI