Restriction map for
pFN21AE3910
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbvCI
BcgI BciVI BfrI BglII BlnI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstYI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MflI MluI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 808 GTCTAC GT^[AC][GT]AC
AccIII 620 TCCGGA T^CCGGA
AcsI 10 GAATTC [GA]^AATT[CT]
Alw44I 1441 GTGCAC G^TGCAC
AlwI 630 GGATC GGATC(4/5)
AocI 496 CCTGAGG CC^T[ACGT]AGG
ApaI 824 GGGCCC GGGCC^C
ApaLI 1441 GTGCAC G^TGCAC
ApoI 10 GAATTC [GA]^AATT[CT]
AspI 1360 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1319 CCCGGG C^[CT]CG[GA]G
BalI 855 TGGCCA TGG^CCA
BbrPI 568 CACGTG CAC^GTG
BclI 1253 TGATCA T^GATCA
BfaI 1729 CTAG C^TAG
BglI 1083 GCCCATCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 171 CTCCAG CTGGAG(16/14)
BsaAI 568 CACGTG [CT]AC^GT[GA]
BsaBI 1224 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 620 TCCGGA [AT]^CCGG[AT]
BseAI 620 TCCGGA T^CCGGA
BsmI 464 GCATTC GAATGC(1/-1)
BsoBI 1319 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1277 TGTACA T^GTACA
BspEI 620 TCCGGA T^CCGGA
BspMI 1079 ACCTGC ACCTGC(4/8)
BsrFI 1451 ACCGGC [GA]^CCGG[CT]
BsrGI 1277 TGTACA T^GTACA
BstEII 1110 GGTGACC G^GT[ACGT]ACC
BstPI 1110 GGTGACC G^GT[ACGT]ACC
Bsu36I 496 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1451 ACCGGC [GA]^CCGG[CT]
DraIII 956 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 103 GACAACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 409 CTCTTC CTCTTC(1/4)
Eco81I 496 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1110 GGTGACC G^GT[ACGT]ACC
EcoRI 10 GAATTC G^AATTC
FauI 1142 GCGGG CCCGC(4/6)
FbaI 1253 TGATCA T^GATCA
HaeII 588 GGCGCT [GA]GCGC^[CT]
HgaI 693 GACGC GACGC(5/10)
Ksp632I 409 CTCTTC CTCTTC(1/4)
MaeI 1729 CTAG C^TAG
MamI 1224 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 606 CAATTG C^AATTG
MluNI 855 TGGCCA TGG^CCA
MroI 620 TCCGGA T^CCGGA
MscI 855 TGGCCA TGG^CCA
MseI 374 TTAA T^TAA
MunI 606 CAATTG C^AATTG
NspI 935 ACATGC [GA]CATG^[CT]
PflFI 1360 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 568 CACGTG CAC^GTG
PmlI 568 CACGTG CAC^GTG
PvuII 1527 CAGCTG CAG^CTG
ScaI 77 AGTACT AGT^ACT
SmaI 1319 CCCGGG CCC^GGG
SspBI 1277 TGTACA T^GTACA
TaqI 348 TCGA T^CGA
Tru9I 374 TTAA T^TAA
Tth111I 1360 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 348 TCGA T^CGA
XmaCI 1319 CCCGGG C^CCGGG
XmaI 1319 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BlpI 418 GCTGAGC GC^T[ACGT]AGC
591 GCTCAGC
Bpu1102I 418 GCTGAGC GC^T[ACGT]AGC
591 GCTCAGC
BsrBI 787 GAGCGG CCGCTC(-3/-3)
1192 GAGCGG
BstAPI 510 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
955 GCACTTAGTGC
CelII 418 GCTGAGC GC^T[ACGT]AGC
591 GCTCAGC
DraII 823 GGGGCCC [GA]G^G[ACGT]CC[CT]
824 GGGCCCT
EcoO109I 823 GGGGCCC [GA]G^G[ACGT]CC[CT]
824 GGGCCCT
HinfI 835 GATTC G^A[ACGT]TC
1749 GATTC
NcoI 1106 CCATGG C^CATGG
1235 CCATGG
PflMI 249 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCACACCATGG
PstI 43 CTGCAG CTGCA^G
1474 CTGCAG
SacI 430 GAGCTC GAGCT^C
925 GAGCTC
SfaNI 1229 GCATC GCATC(5/9)
1272 GCATC
TfiI 835 GATTC G^A[AT]TC
1749 GATTC
Van91I 249 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCACACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp45I Tsp509I TspRI XcmI
XmnI