Restriction map for
pFN21AE4577
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI AocI AscI AseI AsnI
Asp700I AspEI AviII AvrII BcgI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI TfiI XbaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1481 GGTACC G^GTACC
AccII 1855 CGCG CG^CG
AflII 379 CTTAAG C^TTAAG
Alw44I 465 GTGCAC G^TGCAC
ApaLI 465 GTGCAC G^TGCAC
Asp718I 1481 GGTACC G^GTACC
AspI 385 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1469 TGGCCA TGG^CCA
BamHI 207 GGATCC G^GATCC
BbsI 1630 GTCTTC GAAGAC(2/6)
BbvCI 442 CCTCAGC CCTCAGC(-5/-2)
BciVI 1775 GTATCC GTATCC(6/5)
BfrI 379 CTTAAG C^TTAAG
BpuAI 1630 GTCTTC GAAGAC(2/6)
BsaWI 140 ACCGGA [AT]^CCGG[AT]
BsiEI 582 CGGCCG CG[GA][CT]^CG
BsmI 401 GCATTC GAATGC(1/-1)
BspLU11I 1202 ACATGT A^CATGT
BsrBI 99 GAGCGG CCGCTC(-3/-3)
BssSI 1339 CTCGTG CACGAG(-5/-1)
BstEII 795 GGTGACC G^GT[ACGT]ACC
BstPI 795 GGTGACC G^GT[ACGT]ACC
BstUI 1855 CGCG CG^CG
DraIII 984 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 582 CGGCCG C^GGCCG
EclXI 582 CGGCCG C^GGCCG
Eco52I 582 CGGCCG C^GGCCG
EcoO65I 795 GGTGACC G^GT[ACGT]ACC
HgaI 2148 GACGC GACGC(5/10)
KpnI 1481 GGTACC GGTAC^C
KspI 1854 CCGCGG CCGC^GG
MluNI 1469 TGGCCA TGG^CCA
MscI 1469 TGGCCA TGG^CCA
MvnI 1855 CGCG CG^CG
NotI 581 GCGGCCGC GC^GGCCGC
PaeR7I 2200 CTCGAG C^TCGAG
PflFI 385 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1795 GAGCTC GAGCT^C
SacII 1854 CCGCGG CCGC^GG
Tth111I 385 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1994 CCATCTCTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2200 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 145 AAATTC [GA]^AATT[CT]
1804 AAATTC
AcyI 1367 GGCGCC G[GA]^CG[CT]C
1682 GGCGCC
Aor51HI 493 AGCGCT AGC^GCT
2099 AGCGCT
ApaI 830 GGGCCC GGGCC^C
1760 GGGCCC
ApoI 145 AAATTC [GA]^AATT[CT]
1804 AAATTC
AspHI 465 GTGCAC G[AT]GC[AT]^C
1795 GAGCTC
BbeI 1367 GGCGCC GGCGC^C
1682 GGCGCC
BbrPI 1372 CACGTG CAC^GTG
1541 CACGTG
BfaI 754 CTAG C^TAG
1321 CTAG
BsaHI 1367 GGCGCC G[GA]^CG[CT]C
1682 GGCGCC
BsaI 1939 GAGACC GGTCTC(1/5)
246 GGTCTC
BsiHKAI 465 GTGCAC G[AT]GC[AT]^C
1795 GAGCTC
BspHI 1574 TCATGA T^CATGA
1931 TCATGA
BstAPI 933 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2145 GCAGACGCTGC
BstXI 54 CCAAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1811 CCATCGGCATGG
Eco47III 493 AGCGCT AGC^GCT
2099 AGCGCT
Hin1I 1367 GGCGCC G[GA]^CG[CT]C
1682 GGCGCC
HincII 166 GTCAAC GT[CT]^[GA]AC
1945 GTCAAC
HindII 166 GTCAAC GT[CT]^[GA]AC
1945 GTCAAC
KasI 1367 GGCGCC G^GCGCC
1682 GGCGCC
MaeI 754 CTAG C^TAG
1321 CTAG
MaeIII 120 GTCAC ^GT[ACGT]AC
796 GTGAC
MseI 380 TTAA T^TAA
1177 TTAA
MslI 1440 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1812 CATCGGCATG
NarI 1367 GGCGCC GG^CGCC
1682 GGCGCC
NcoI 113 CCATGG C^CATGG
1730 CCATGG
PflMI 656 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1472 CCATCGAGTGG
PmaCI 1372 CACGTG CAC^GTG
1541 CACGTG
PmlI 1372 CACGTG CAC^GTG
1541 CACGTG
PpuMI 1598 GGGACCT [GA]G^G[AT]CC[CT]
2168 AGGACCC
RcaI 1574 TCATGA T^CATGA
1931 TCATGA
SapI 234 GCTCTTC GCTCTTC(1/4)
1797 GCTCTTC
SbfI 711 CCTGCAGG CCTGCA^GG
873 CCTGCAGG
SfoI 1367 GGCGCC GGC^GCC
1682 GGCGCC
SmaI 251 CCCGGG CCC^GGG
572 CCCGGG
SphI 567 GCATGC GCATG^C
747 GCATGC
Sse8387I 711 CCTGCAGG CCTGCA^GG
873 CCTGCAGG
Tru9I 380 TTAA T^TAA
1177 TTAA
Tsp45I 120 GTCAC ^GT[GC]AC
796 GTGAC
Tsp509I 146 AATT ^AATT
1805 AATT
Van91I 656 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1472 CCATCGAGTGG
XmaCI 251 CCCGGG C^CCGGG
572 CCCGGG
XmaI 251 CCCGGG C^CCGGG
572 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI TspRI
TthHB8I XhoII