Restriction map for
pFN21AE4490
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# NO CUT ENZYMES ( 107 enzymes) #
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Acc65I AccI AccIII AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AviII AvrII BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 206 GACGTC GACGT^C
AclI 735 AACGTT AA^CGTT
AseI 288 ATTAAT AT^TAAT
AsnI 288 ATTAAT AT^TAAT
Asp700I 723 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 249 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 577 CCCGAG C^[CT]CG[GA]G
BamHI 953 GGATCC G^GATCC
BbeI 100 GGCGCC GGCGC^C
BbsI 1018 GTCTTC GAAGAC(2/6)
BcnI 669 CCCGG CC^[GC]GG
BfaI 1357 CTAG C^TAG
BglII 485 AGATCT A^GATCT
BlpI 651 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 651 GCTGAGC GC^T[ACGT]AGC
BpuAI 1018 GTCTTC GAAGAC(2/6)
BsgI 796 GTGCAG GTGCAG(16/14)
BsiEI 1252 CGGCCG CG[GA][CT]^CG
BsoBI 577 CCCGAG C^[CT]CG[GA]G
BsrFI 1254 GCCGGC [GA]^CCGG[CT]
BssSI 82 CTCGTG CACGAG(-5/-1)
BstEII 864 GGTCACC G^GT[ACGT]ACC
BstPI 864 GGTCACC G^GT[ACGT]ACC
BstXI 894 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 651 GCTGAGC GC^T[ACGT]AGC
CfoI 101 GCGC GCG^C
Cfr10I 1254 GCCGGC [GA]^CCGG[CT]
DrdI 1495 GACTCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1252 CGGCCG C^GGCCG
EclXI 1252 CGGCCG C^GGCCG
Eco52I 1252 CGGCCG C^GGCCG
EcoO65I 864 GGTCACC G^GT[ACGT]ACC
FauI 97 GCGGG CCCGC(4/6)
HaeII 100 GGCGCC [GA]GCGC^[CT]
HhaI 101 GCGC GCG^C
HinP1I 101 GCGC G^CGC
HincII 1282 GTCAAC GT[CT]^[GA]AC
HindII 1282 GTCAAC GT[CT]^[GA]AC
KasI 100 GGCGCC G^GCGCC
MaeI 1357 CTAG C^TAG
MseI 289 TTAA T^TAA
MslI 543 CATTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1254 GCCGGC GCC^GGC
NarI 100 GGCGCC GG^CGCC
NciI 669 CCCGG CC^[GC]GG
NcoI 881 CCATGG C^CATGG
NgoMIV 1254 GCCGGC G^CCGGC
PflFI 249 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 288 ATTAAT AT^TAAT
Psp1406I 735 AACGTT AA^CGTT
SacI 839 GAGCTC GAGCT^C
SbfI 1122 CCTGCAGG CCTGCA^GG
SfoI 100 GGCGCC GGC^GCC
SmlI 984 CTTGAG C^T[CT][GA]AG
Sse8387I 1122 CCTGCAGG CCTGCA^GG
TfiI 43 GATTC G^A[AT]TC
Tru9I 289 TTAA T^TAA
Tth111I 249 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 723 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 96 CGCG CG^CG
793 CGCG
AlwNI 562 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
646 CAGCTGCTG
BanI 100 GGCGCC G^G[CT][GA]CC
1276 GGCACC
BpmI 388 CTCCAG CTGGAG(16/14)
826 CTGGAG
BsaI 1053 GAGACC GGTCTC(1/5)
1414 GAGACC
BsmI 147 GAATGC GAATGC(1/-1)
1166 GCATTC
BsrDI 368 CATTGC GCAATG(2/0)
1269 CATTGC
BstAPI 327 GCACACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
355 GCAATTTCTGC
BstF5I 807 CATCC GGATG(2/0)
1209 CATCC
BstUI 96 CGCG CG^CG
793 CGCG
DsaI 504 CCGTGG C^C[GA][CT]GG
881 CCATGG
EcoT14I 494 CCAAGG C^C[AT][AT]GG
881 CCATGG
FokI 807 CATCC GGATG(9/13)
1209 CATCC
HapII 670 CCGG C^CGG
1255 CCGG
HgaI 422 GACGC GACGC(5/10)
449 GCGTC
HpaII 670 CCGG C^CGG
1255 CCGG
MspI 670 CCGG C^CGG
1255 CCGG
MvnI 96 CGCG CG^CG
793 CGCG
PstI 1123 CTGCAG CTGCA^G
1191 CTGCAG
PvuII 646 CAGCTG CAG^CTG
1499 CAGCTG
SfcI 1123 CTGCAG C^T[GA][CT]AG
1191 CTGCAG
StuI 170 AGGCCT AGG^CCT
1528 AGGCCT
StyI 494 CCAAGG C^C[AT][AT]GG
881 CCATGG
TaqI 1001 TCGA T^CGA
1493 TCGA
TthHB8I 1001 TCGA T^CGA
1493 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BalI BanII BbvI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
HaeIII Hin1I HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI XhoII