Restriction map for
pFN21AE3931
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 463 GTAGAC GT^[AC][GT]AC
Alw44I 1546 GTGCAC G^TGCAC
AlwI 234 GGATC GGATC(4/5)
AocI 177 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1145 AGCGCT AGC^GCT
ApaLI 1546 GTGCAC G^TGCAC
Asp700I 447 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 241 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 347 TGGCCA TGG^CCA
BbeI 1508 GGCGCC GGCGC^C
BlpI 1452 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1452 GCTGAGC GC^T[ACGT]AGC
BsaI 868 GGTCTC GGTCTC(1/5)
BsmBI 745 CGTCTC CGTCTC(1/5)
BstAPI 1368 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 234 GGATCT [GA]^GATC[CT]
Bsu36I 177 CCTGAGG CC^T[ACGT]AGG
CelII 1452 GCTGAGC GC^T[ACGT]AGC
DraIII 517 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1145 AGCGCT AGC^GCT
Eco81I 177 CCTGAGG CC^T[ACGT]AGG
EcoNI 388 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 757 ATGCAT ATGCA^T
KasI 1508 GGCGCC G^GCGCC
KspI 788 CCGCGG CCGC^GG
MflI 234 GGATCT [GA]^GATC[CT]
MluNI 347 TGGCCA TGG^CCA
MscI 347 TGGCCA TGG^CCA
NarI 1508 GGCGCC GG^CGCC
NsiI 757 ATGCAT ATGCA^T
NspI 289 ACATGC [GA]CATG^[CT]
PflFI 241 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 382 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1533 GACTC GAGTC(4/5)
PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1431 GAGCTC GAGCT^C
SacII 788 CCGCGG CCGC^GG
SfoI 1508 GGCGCC GGC^GCC
Tth111I 241 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 382 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 237 TCTAGA T^CTAGA
XcmI 335 CCAACCTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 234 GGATCT [GA]^GATC[CT]
XmnI 447 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 743 GGCGTC G[GA]^CG[CT]C
1508 GGCGCC
AlwNI 1369 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1444 CAGACGCTG
ApaI 1175 GGGCCC GGGCC^C
1284 GGGCCC
BbrPI 574 CACGTG CAC^GTG
1543 CACGTG
BbsI 10 GAAGAC GAAGAC(2/6)
136 GAAGAC
BpuAI 10 GAAGAC GAAGAC(2/6)
136 GAAGAC
BsaAI 574 CACGTG [CT]AC^GT[GA]
1543 CACGTG
BsaHI 743 GGCGTC G[GA]^CG[CT]C
1508 GGCGCC
BsgI 544 GTGCAG GTGCAG(16/14)
607 GTGCAG
BsiEI 1154 CGGCCG CG[GA][CT]^CG
1194 CGGCCG
BsrFI 1196 GCCGGC [GA]^CCGG[CT]
1349 GCCGGC
BstXI 476 CCATCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
934 CCAGAGGTCTGG
Cfr10I 1196 GCCGGC [GA]^CCGG[CT]
1349 GCCGGC
DpnII 235 GATC ^GATC
412 GATC
DsaI 788 CCGCGG C^C[GA][CT]GG
1301 CCGTGG
EagI 1154 CGGCCG C^GGCCG
1194 CGGCCG
EclXI 1154 CGGCCG C^GGCCG
1194 CGGCCG
Eco52I 1154 CGGCCG C^GGCCG
1194 CGGCCG
Hin1I 743 GGCGTC G[GA]^CG[CT]C
1508 GGCGCC
MaeII 575 ACGT A^CGT
1544 ACGT
MboI 235 GATC ^GATC
412 GATC
MslI 477 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
967 CATAGCCATG
NaeI 1196 GCCGGC GCC^GGC
1349 GCCGGC
NdeII 235 GATC ^GATC
412 GATC
NgoMIV 1196 GCCGGC G^CCGGC
1349 GCCGGC
PmaCI 574 CACGTG CAC^GTG
1543 CACGTG
PmlI 574 CACGTG CAC^GTG
1543 CACGTG
Sau3AI 235 GATC ^GATC
412 GATC
TaqI 41 TCGA T^CGA
393 TCGA
TthHB8I 41 TCGA T^CGA
393 TCGA
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PstI
PvuII RsaI SapI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I TspRI