Restriction map for
pFN21AE1335
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI Ksp632I MaeI MaeII MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TfiI Tsp509I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 104 GGCGCC G[GA]^CG[CT]C
AflII 505 CTTAAG C^TTAAG
AlwI 675 GGATC GGATC(4/5)
BanI 104 GGCGCC G^G[CT][GA]CC
BbeI 104 GGCGCC GGCGC^C
BbsI 648 GTCTTC GAAGAC(2/6)
BfrI 505 CTTAAG C^TTAAG
BglI 369 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 648 GTCTTC GAAGAC(2/6)
BsaHI 104 GGCGCC G[GA]^CG[CT]C
BsaI 520 GAGACC GGTCTC(1/5)
BsgI 129 GTGCAG GTGCAG(16/14)
BsmFI 688 GTCCC GGGAC(10/14)
Bsp1407I 146 TGTACA T^GTACA
BsrGI 146 TGTACA T^GTACA
BstAPI 345 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 675 GGATCT [GA]^GATC[CT]
DdeI 496 CTGAG C^T[ACGT]AG
DrdI 529 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 366 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 610 GACGC GACGC(5/10)
Hin1I 104 GGCGCC G[GA]^CG[CT]C
HindIII 286 AAGCTT A^AGCTT
KasI 104 GGCGCC G^GCGCC
KspI 135 CCGCGG CCGC^GG
MflI 675 GGATCT [GA]^GATC[CT]
MseI 506 TTAA T^TAA
MslI 162 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 104 GGCGCC GG^CGCC
PflMI 524 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 641 AGGACCC [GA]G^G[AT]CC[CT]
PstI 262 CTGCAG CTGCA^G
PvuII 265 CAGCTG CAG^CTG
SacII 135 CCGCGG CCGC^GG
SfcI 262 CTGCAG C^T[GA][CT]AG
SfoI 104 GGCGCC GGC^GCC
SmlI 505 CTTAAG C^T[CT][GA]AG
SspBI 146 TGTACA T^GTACA
Tru9I 506 TTAA T^TAA
Van91I 524 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 668 CCAAGCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 675 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 61 CGCG CG^CG
136 CGCG
AvaII 417 GGACC G^G[AT]CC
642 GGACC
AviII 278 TGCGCA TGC^GCA
320 TGCGCA
BalI 186 TGGCCA TGG^CCA
326 TGGCCA
BcnI 117 CCCGG CC^[GC]GG
486 CCCGG
BseRI 178 CTCCTC GAGGAG(10/8)
9 GAGGAG
BsmAI 498 GAGAC GTCTC(1/5)
520 GAGAC
BsmI 387 GAATGC GAATGC(1/-1)
441 GAATGC
BsrFI 101 GCCGGC [GA]^CCGG[CT]
227 GCCGGC
BstUI 61 CGCG CG^CG
136 CGCG
Cfr10I 101 GCCGGC [GA]^CCGG[CT]
227 GCCGGC
DpnII 34 GATC ^GATC
676 GATC
EaeI 186 TGGCCA [CT]^GGCC[GA]
326 TGGCCA
FauI 333 CCCGC CCCGC(4/6)
465 CCCGC
FspI 278 TGCGCA TGC^GCA
320 TGCGCA
HaeII 104 GGCGCC [GA]GCGC^[CT]
348 GGCGCT
HinfI 171 GACTC G^A[ACGT]TC
625 GAGTC
HphI 168 GGTGA GGTGA(8/7)
248 TCACC
MboI 34 GATC ^GATC
676 GATC
MboII 31 GAAGA GAAGA(8/7)
649 TCTTC
MluNI 186 TGGCCA TGG^CCA
326 TGGCCA
MscI 186 TGGCCA TGG^CCA
326 TGGCCA
MvnI 61 CGCG CG^CG
136 CGCG
NaeI 101 GCCGGC GCC^GGC
227 GCCGGC
NciI 117 CCCGG CC^[GC]GG
486 CCCGG
NcoI 356 CCATGG C^CATGG
515 CCATGG
NdeII 34 GATC ^GATC
676 GATC
NgoMIV 101 GCCGGC G^CCGGC
227 GCCGGC
PleI 171 GACTC GAGTC(4/5)
625 GAGTC
Sau3AI 34 GATC ^GATC
676 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI