Restriction map for
pFN21AE5716
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI Alw44I AocI
ApaLI AscI AseI AsnI Asp718I
AviII BalI BbeI BbrPI BbsI
BcgI BciVI BfrI BglII BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstUI BstZ17I
Bsu36I CpoI DraI DraIII DrdI
Eco81I EcoNI EcoT22I FseI FspI
HgaI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 392 GACGTC GACGT^C
AcyI 392 GACGTC G[GA]^CG[CT]C
AfaI 1494 GTAC GT^AC
AhdI 154 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1061 AGCGCT AGC^GCT
ApaI 848 GGGCCC GGGCC^C
Asp700I 12 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 154 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 514 GAGCAC G[AT]GC[AT]^C
AspI 565 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1264 CCTAGG C^CTAGG
BamHI 795 GGATCC G^GATCC
BanI 1582 GGCACC G^G[CT][GA]CC
BclI 1127 TGATCA T^GATCA
BlnI 1264 CCTAGG C^CTAGG
BlpI 1677 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1677 GCTGAGC GC^T[ACGT]AGC
BsaHI 392 GACGTC G[GA]^CG[CT]C
BsgI 1357 GTGCAG GTGCAG(16/14)
BsiEI 1533 CGGCCG CG[GA][CT]^CG
BsiHKAI 514 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1493 TGTACA T^GTACA
BspDI 236 ATCGAT AT^CGAT
BsrDI 1033 GCAATG GCAATG(2/0)
BsrFI 318 GCCGGT [GA]^CCGG[CT]
BsrGI 1493 TGTACA T^GTACA
BssSI 218 CACGAG CACGAG(-5/-1)
BstAPI 1359 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1080 GGTGACC G^GT[ACGT]ACC
BstPI 1080 GGTGACC G^GT[ACGT]ACC
CelII 1677 GCTGAGC GC^T[ACGT]AGC
Cfr10I 318 GCCGGT [GA]^CCGG[CT]
ClaI 236 ATCGAT AT^CGAT
DsaI 1607 CCACGG C^C[GA][CT]GG
EagI 1533 CGGCCG C^GGCCG
Eam1105I 154 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1533 CGGCCG C^GGCCG
Eco47III 1061 AGCGCT AGC^GCT
Eco52I 1533 CGGCCG C^GGCCG
EcoO65I 1080 GGTGACC G^GT[ACGT]ACC
EcoRI 1349 GAATTC G^AATTC
EcoRV 586 GATATC GAT^ATC
FbaI 1127 TGATCA T^GATCA
HaeII 1061 AGCGCT [GA]GCGC^[CT]
Hin1I 392 GACGTC G[GA]^CG[CT]C
MseI 188 TTAA T^TAA
PflFI 565 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 1494 GTAC GT^AC
SapI 1672 GAAGAGC GCTCTTC(1/4)
SfaNI 1717 GATGC GCATC(5/9)
SmlI 909 CTTGAG C^T[CT][GA]AG
SspBI 1493 TGTACA T^GTACA
StuI 1239 AGGCCT AGG^CCT
Tru9I 188 TTAA T^TAA
Tth111I 565 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 12 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 606 GAATTT [GA]^AATT[CT]
1349 GAATTC
ApoI 606 GAATTT [GA]^AATT[CT]
1349 GAATTC
BanII 848 GGGCCC G[GA]GC[CT]^C
1315 GAGCCC
BfaI 800 CTAG C^TAG
1265 CTAG
BglI 1471 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1775 GCCTGGCTGGC
BsaI 1603 GAGACC GGTCTC(1/5)
1747 GAGACC
BsrBI 61 GAGCGG CCGCTC(-3/-3)
124 GAGCGG
BstXI 887 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1607 CCACGGAGCTGG
EaeI 1533 CGGCCG [CT]^GGCC[GA]
1782 TGGCCG
MaeI 800 CTAG C^TAG
1265 CTAG
MslI 888 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1323 CACTGCTGTG
NlaIII 478 CATG CATG^
530 CATG
PpuMI 776 GGGACCC [GA]G^G[AT]CC[CT]
1394 AGGACCC
PvuII 538 CAGCTG CAG^CTG
915 CAGCTG
SfcI 313 CTACAG C^T[GA][CT]AG
1566 CTACAG
SmaI 299 CCCGGG CCC^GGG
1173 CCCGGG
TaqI 237 TCGA T^CGA
390 TCGA
TfiI 474 GATTC G^A[AT]TC
1027 GATTC
Tsp509I 607 AATT ^AATT
1350 AATT
TthHB8I 237 TCGA T^CGA
390 TCGA
XmaCI 299 CCCGGG C^CCGGG
1173 CCCGGG
XmaI 299 CCCGGG C^CCGGG
1173 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
AvaII BbvCI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI StyI
TseI Tsp45I TspRI XcmI XhoII