Restriction map for
pFN21AE4375
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwI AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BsaAI
BsaI BseAI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MluI MroI MseI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 721 GTCTAC GT^[AC][GT]AC
AcyI 1072 GGCGTC G[GA]^CG[CT]C
AgeI 9 ACCGGT A^CCGGT
AocI 498 CCTCAGG CC^T[ACGT]AGG
ApaI 659 GGGCCC GGGCC^C
AspI 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 690 GGTGCC G^G[CT][GA]CC
BglII 95 AGATCT A^GATCT
BlpI 875 GCTCAGC GC^T[ACGT]AGC
BpmI 643 CTGGAG CTGGAG(16/14)
Bpu1102I 875 GCTCAGC GC^T[ACGT]AGC
BsaBI 127 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1072 GGCGTC G[GA]^CG[CT]C
BsaWI 9 ACCGGT [AT]^CCGG[AT]
BsgI 619 GTGCAG GTGCAG(16/14)
BsiEI 1066 CGGCCG CG[GA][CT]^CG
BspMI 728 ACCTGC ACCTGC(4/8)
BsrBI 873 CCGCTC CCGCTC(-3/-3)
BstYI 95 AGATCT [GA]^GATC[CT]
Bsu36I 498 CCTCAGG CC^T[ACGT]AGG
CelII 875 GCTCAGC GC^T[ACGT]AGC
EagI 1066 CGGCCG C^GGCCG
EclXI 1066 CGGCCG C^GGCCG
Eco52I 1066 CGGCCG C^GGCCG
Eco81I 498 CCTCAGG CC^T[ACGT]AGG
HaeII 503 GGCGCT [GA]GCGC^[CT]
Hin1I 1072 GGCGTC G[GA]^CG[CT]C
HindIII 1174 AAGCTT A^AGCTT
MaeII 314 ACGT A^CGT
MamI 127 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 95 AGATCT [GA]^GATC[CT]
NaeI 693 GCCGGC GCC^GGC
NgoMIV 693 GCCGGC G^CCGGC
PflFI 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 237 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 9 ACCGGT A^CCGGT
PpuMI 759 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 730 CTGCAG CTGCA^G
SfcI 730 CTGCAG C^T[GA][CT]AG
SmlI 136 CTCAAG C^T[CT][GA]AG
TaqI 1058 TCGA T^CGA
Tth111I 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1058 TCGA T^CGA
Van91I 237 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 149 CCAATGCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 95 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
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AfaI 561 GTAC GT^AC
702 GTAC
AviII 593 TGCGCA TGC^GCA
653 TGCGCA
BalI 221 TGGCCA TGG^CCA
902 TGGCCA
FspI 593 TGCGCA TGC^GCA
653 TGCGCA
HgaI 359 GCGTC GACGC(5/10)
1073 GCGTC
MluNI 221 TGGCCA TGG^CCA
902 TGGCCA
MscI 221 TGGCCA TGG^CCA
902 TGGCCA
NcoI 224 CCATGG C^CATGG
1156 CCATGG
RsaI 561 GTAC GT^AC
702 GTAC
SphI 446 GCATGC GCATG^C
1123 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AvaII BanII BbsI
BbvI BcnI BglI BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI