##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MnlI        MroI        MscI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        TaqI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AciI          138  CCGC                 CCGC(-3/-1)
BamHI         126  GGATCC               G^GATCC
BpmI          262  CTGGAG               CTGGAG(16/14)
BsaJI         130  CCATGG               C^C[ACGT][ACGT]GG
BsrDI         140  GCAATG               GCAATG(2/0)
BssKI         261  CCTGG                ^CC[ACGT]GG
BstNI         261  CCTGG                CC^[AT]GG
BstXI           6  CCATGAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         126  GGATCC               [GA]^GATC[CT]
Cac8I         105  GCTGGC               GC[ACGT]^[ACGT]GC
DdeI          121  CTAAG                C^T[ACGT]AG
DpnII         127  GATC                 ^GATC
DraI          202  TTTAAA               TTT^AAA
DsaI          130  CCATGG               C^C[GA][CT]GG
EcoRII        261  CCTGG                ^CC[AT]GG
EcoT14I       130  CCATGG               C^C[AT][AT]GG
HindIII       146  AAGCTT               A^AGCTT
MboI          127  GATC                 ^GATC
MflI          126  GGATCC               [GA]^GATC[CT]
MseI          203  TTAA                 T^TAA
MvaI          261  CCTGG                CC^[AT]GG
MwoI          241  GCTGTAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          130  CCATGG               C^CATGG
NdeII         127  GATC                 ^GATC
PleI          265  GAGTC                GAGTC(4/5)
Sau3AI        127  GATC                 ^GATC
ScrFI         261  CCTGG                CC^[ACGT]GG
SfaNI         288  GCATC                GCATC(5/9)
SfcI          242  CTGTAG               C^T[GA][CT]AG
SspI          254  AATATT               AAT^ATT
StyI          130  CCATGG               C^C[AT][AT]GG
TfiI          209  GATTC                G^A[AT]TC
Tru9I         203  TTAA                 T^TAA
XhoII         126  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwI          127  GATCC                GGATC(4/5)
              126  GGATC               
BbvI          109  GCTGC                GCAGC(8/12)
              226  GCTGC               
BsmFI          76  GGGAC                GGGAC(10/14)
              267  GTCCC               
Cfr13I          3  GGCCC                G^G[ACGT]CC
              135  GGGCC               
HaeIII          3  GGCC                 GG^CC
              136  GGCC                
HinfI         209  GATTC                G^A[ACGT]TC
              265  GAGTC               
MboII          66  GAAGA                GAAGA(8/7)
               99  GAAGA               
NlaIV         126  GGATCC               GG[ACGT]^[ACGT]CC
              134  GGGGCC              
Sau96I          3  GGCCC                G^G[ACGT]CC
              135  GGGCC               
TseI          109  GCTGC                G^C[AT]GC
              226  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AluI        Fnu4HI      ItaI        MslI        NlaIII