Restriction map for
pFN21AE1421
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BbrPI BbvCI BciVI
BglII BsaAI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BsrI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI Ksp632I MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tsp509I TspRI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1273 GTCGAC GT^[AC][GT]AC
AfaI 1378 GTAC GT^AC
AflII 903 CTTAAG C^TTAAG
Alw44I 1318 GTGCAC G^TGCAC
Aor51HI 804 AGCGCT AGC^GCT
ApaI 29 GGGCCC GGGCC^C
ApaLI 1318 GTGCAC G^TGCAC
AvrII 783 CCTAGG C^CTAGG
BamHI 1056 GGATCC G^GATCC
BbsI 721 GTCTTC GAAGAC(2/6)
BcgI 1072 CGAGCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 425 TGATCA T^GATCA
BfaI 784 CTAG C^TAG
BfrI 903 CTTAAG C^TTAAG
BlnI 783 CCTAGG C^CTAGG
BpuAI 721 GTCTTC GAAGAC(2/6)
BsaBI 225 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 259 GAGACC GGTCTC(1/5)
BsgI 1201 CTGCAC GTGCAG(16/14)
BsiEI 1386 CGACCG CG[GA][CT]^CG
BsmBI 131 GAGACG CGTCTC(1/5)
BsmI 345 GCATTC GAATGC(1/-1)
BspMI 390 ACCTGC ACCTGC(4/8)
BsrFI 1103 GCCGGC [GA]^CCGG[CT]
BstEII 915 GGTCACC G^GT[ACGT]ACC
BstPI 915 GGTCACC G^GT[ACGT]ACC
Cfr10I 1103 GCCGGC [GA]^CCGG[CT]
Eco47III 804 AGCGCT AGC^GCT
EcoNI 1362 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 915 GGTCACC G^GT[ACGT]ACC
FbaI 425 TGATCA T^GATCA
HgaI 759 GACGC GACGC(5/10)
HincII 1273 GTCGAC GT[CT]^[GA]AC
HindII 1273 GTCGAC GT[CT]^[GA]AC
KspI 992 CCGCGG CCGC^GG
MaeI 784 CTAG C^TAG
MamI 225 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 722 TCTTC GAAGA(8/7)
MslI 219 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1103 GCCGGC GCC^GGC
NcoI 815 CCATGG C^CATGG
NgoMIV 1103 GCCGGC G^CCGGC
NspI 403 GCATGT [GA]CATG^[CT]
PstI 1363 CTGCAG CTGCA^G
RsaI 1378 GTAC GT^AC
SacII 992 CCGCGG CCGC^GG
SalI 1273 GTCGAC G^TCGAC
SbfI 1362 CCTGCAGG CCTGCA^GG
SexAI 911 ACCTGGT A^CC[AT]GGT
SfcI 1363 CTGCAG C^T[GA][CT]AG
SmlI 903 CTTAAG C^T[CT][GA]AG
Sse8387I 1362 CCTGCAGG CCTGCA^GG
StuI 317 AGGCCT AGG^CCT
XcmI 350 CCACAGGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BalI 227 TGGCCA TGG^CCA
310 TGGCCA
BglI 394 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
982 GCCCGGGTGGC
BlpI 945 GCTCAGC GC^T[ACGT]AGC
1338 GCTCAGC
Bpu1102I 945 GCTCAGC GC^T[ACGT]AGC
1338 GCTCAGC
BsmAI 131 GAGAC GTCTC(1/5)
259 GAGAC
BssHII 194 GCGCGC G^CGCGC
1391 GCGCGC
BssSI 1204 CACGAG CACGAG(-5/-1)
667 CTCGTG
BstAPI 519 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1107 GCAGCTGCTGC
BstXI 218 CCATGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
313 CCAGAGGCCTGG
BstYI 249 GGATCT [GA]^GATC[CT]
1056 GGATCC
CelII 945 GCTCAGC GC^T[ACGT]AGC
1338 GCTCAGC
MflI 249 GGATCT [GA]^GATC[CT]
1056 GGATCC
MluNI 227 TGGCCA TGG^CCA
310 TGGCCA
MscI 227 TGGCCA TGG^CCA
310 TGGCCA
MseI 701 TTAA T^TAA
904 TTAA
PflMI 276 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
846 CCACGCCCTGG
PleI 963 GACTC GAGTC(4/5)
475 GAGTC
PpuMI 908 GGGACCT [GA]G^G[AT]CC[CT]
1300 GGGACCC
SacI 1207 GAGCTC GAGCT^C
1423 GAGCTC
SfaNI 516 GATGC GCATC(5/9)
1035 GATGC
TaqI 1028 TCGA T^CGA
1274 TCGA
TfiI 488 GATTC G^A[AT]TC
587 GAATC
Tru9I 701 TTAA T^TAA
904 TTAA
TthHB8I 1028 TCGA T^CGA
1274 TCGA
Van91I 276 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
846 CCACGCCCTGG
XhoII 249 GGATCT [GA]^GATC[CT]
1056 GGATCC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AspI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflFI PvuII Sau3AI Sau96I ScrFI
SfoI SmaI StyI TseI Tsp45I
Tth111I XmaCI XmaI