Restriction map for
pFN21AE5217
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrFI BsrGI
BsrI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SspBI
SspI StuI SwaI TfiI Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 388 CGCG CG^CG
AccIII 488 TCCGGA T^CCGGA
AfaI 141 GTAC GT^AC
Alw44I 462 GTGCAC G^TGCAC
AlwNI 328 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 462 GTGCAC G^TGCAC
AscI 386 GGCGCGCC GG^CGCGCC
AvaI 362 CCCGGG C^[CT]CG[GA]G
AviII 269 TGCGCA TGC^GCA
BalI 596 TGGCCA TGG^CCA
BpmI 586 CTGGAG CTGGAG(16/14)
BsaI 37 GAGACC GGTCTC(1/5)
BsaWI 488 TCCGGA [AT]^CCGG[AT]
BseAI 488 TCCGGA T^CCGGA
BseRI 549 CTCCTC GAGGAG(10/8)
BsiEI 237 CGGCCG CG[GA][CT]^CG
BsiYI 321 CCCTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 321 CCCTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 402 GAGACG CGTCTC(1/5)
BsoBI 362 CCCGGG C^[CT]CG[GA]G
BspEI 488 TCCGGA T^CCGGA
BspHI 260 TCATGA T^CATGA
BssHII 387 GCGCGC G^CGCGC
BssSI 473 CTCGTG CACGAG(-5/-1)
BstF5I 417 GGATG GGATG(2/0)
BstUI 388 CGCG CG^CG
DraII 329 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 237 CGGCCG C^GGCCG
EclXI 237 CGGCCG C^GGCCG
Eco52I 237 CGGCCG C^GGCCG
EcoO109I 329 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 542 CCAAGG C^C[AT][AT]GG
FokI 417 GGATG GGATG(9/13)
FspI 269 TGCGCA TGC^GCA
HinfI 370 GACTC G^A[ACGT]TC
HphI 336 GGTGA GGTGA(8/7)
MboII 573 GAAGA GAAGA(8/7)
MluNI 596 TGGCCA TGG^CCA
MroI 488 TCCGGA T^CCGGA
MscI 596 TGGCCA TGG^CCA
MspA1I 26 CAGCGG C[AC]G^C[GT]G
MvnI 388 CGCG CG^CG
NspI 282 GCATGC [GA]CATG^[CT]
PleI 370 GACTC GAGTC(4/5)
PpuMI 329 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 260 TCATGA T^CATGA
RsaI 141 GTAC GT^AC
SacI 526 GAGCTC GAGCT^C
SbfI 102 CCTGCAGG CCTGCA^GG
SmaI 362 CCCGGG CCC^GGG
SphI 282 GCATGC GCATG^C
Sse8387I 102 CCTGCAGG CCTGCA^GG
StyI 542 CCAAGG C^C[AT][AT]GG
XmaCI 362 CCCGGG C^CCGGG
XmaI 362 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AvaII 330 GGTCC G^G[AT]CC
358 GGTCC
BanII 526 GAGCTC G[GA]GC[CT]^C
546 GGGCTC
BsgI 82 GTGCAG GTGCAG(16/14)
155 GTGCAG
BsrDI 114 CATTGC GCAATG(2/0)
504 CATTGC
Cac8I 282 GCATGC GC[ACGT]^[ACGT]GC
387 GCGCGC
Cfr13I 330 GGTCC G^G[ACGT]CC
358 GGTCC
DdeI 191 CTCAG C^T[ACGT]AG
509 CTGAG
DpnII 87 GATC ^GATC
294 GATC
EaeI 237 CGGCCG [CT]^GGCC[GA]
596 TGGCCA
HaeII 209 AGCGCC [GA]GCGC^[CT]
469 GGCGCT
MboI 87 GATC ^GATC
294 GATC
MseI 497 TTAA T^TAA
569 TTAA
NdeII 87 GATC ^GATC
294 GATC
NlaIV 358 GGTCCC GG[ACGT]^[ACGT]CC
547 GGCTCC
PstI 103 CTGCAG CTGCA^G
325 CTGCAG
Sau3AI 87 GATC ^GATC
294 GATC
Sau96I 330 GGTCC G^G[ACGT]CC
358 GGTCC
SfaNI 231 GATGC GCATC(5/9)
272 GCATC
SfcI 103 CTGCAG C^T[GA][CT]AG
325 CTGCAG
TaqI 290 TCGA T^CGA
553 TCGA
Tru9I 497 TTAA T^TAA
569 TTAA
TthHB8I 290 TCGA T^CGA
553 TCGA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AspHI BbvI BcnI
BmyI BsaJI BsiHKAI BsmAI BsmFI
Bsp1286I BspMI BssKI BstNI CfoI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MslI
MspI MvaI MwoI NciI NlaIII
ScrFI TseI TspRI