Restriction map for
pFN21AE4535
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AfaI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BseRI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRV FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 200 GTAGAC GT^[AC][GT]AC
AciI 778 GCGG CCGC(-3/-1)
AclI 810 AACGTT AA^CGTT
AflIII 459 ACATGT A^C[GA][CT]GT
AlwNI 223 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 562 CCTGAGG CC^T[ACGT]AGG
AvaI 485 CTCGGG C^[CT]CG[GA]G
AvaII 955 GGTCC G^G[AT]CC
AvrII 534 CCTAGG C^CTAGG
BalI 1372 TGGCCA TGG^CCA
BamHI 757 GGATCC G^GATCC
BanI 529 GGTGCC G^G[CT][GA]CC
BlnI 534 CCTAGG C^CTAGG
BpmI 569 CTCCAG CTGGAG(16/14)
BsaAI 1625 TACGTG [CT]AC^GT[GA]
BsgI 677 GTGCAG GTGCAG(16/14)
BsiEI 959 CGACCG CG[GA][CT]^CG
BsmI 252 GAATGC GAATGC(1/-1)
BsoBI 485 CTCGGG C^[CT]CG[GA]G
BspLU11I 459 ACATGT A^CATGT
BstAPI 1498 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 562 CCTGAGG CC^T[ACGT]AGG
Cfr13I 955 GGTCC G^G[ACGT]CC
EaeI 1372 TGGCCA [CT]^GGCC[GA]
Eco81I 562 CCTGAGG CC^T[ACGT]AGG
EcoRI 173 GAATTC G^AATTC
HincII 1602 GTCAAC GT[CT]^[GA]AC
HindII 1602 GTCAAC GT[CT]^[GA]AC
MluNI 1372 TGGCCA TGG^CCA
MscI 1372 TGGCCA TGG^CCA
NcoI 312 CCATGG C^CATGG
Psp1406I 810 AACGTT AA^CGTT
SapI 604 GCTCTTC GCTCTTC(1/4)
Sau96I 955 GGTCC G^G[ACGT]CC
SfcI 519 CTATAG C^T[GA][CT]AG
SphI 59 GCATGC GCATG^C
StuI 1244 AGGCCT AGG^CCT
XbaI 841 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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Asp700I 719 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
895 GAAATTCTTC
AspHI 1082 GTGCTC G[AT]GC[AT]^C
1628 GTGCTC
BbsI 352 GAAGAC GAAGAC(2/6)
1558 GTCTTC
BbvI 631 GCAGC GCAGC(8/12)
685 GCAGC
BclI 591 TGATCA T^GATCA
1218 TGATCA
BpuAI 352 GAAGAC GAAGAC(2/6)
1558 GTCTTC
BsaI 1224 GAGACC GGTCTC(1/5)
1801 GGTCTC
BsiHKAI 1082 GTGCTC G[AT]GC[AT]^C
1628 GTGCTC
DraI 1018 TTTAAA TTT^AAA
1143 TTTAAA
DrdI 231 GACCAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
933 GACTCCTTTGTC
EarI 605 CTCTTC CTCTTC(1/4)
1439 GAAGAG
FbaI 591 TGATCA T^GATCA
1218 TGATCA
Fnu4HI 631 GCAGC GC^[ACGT]GC
685 GCAGC
ItaI 631 GCAGC GC^[ACGT]GC
685 GCAGC
Ksp632I 605 CTCTTC CTCTTC(1/4)
1439 GAAGAG
MaeII 811 ACGT A^CGT
1626 ACGT
NdeI 21 CATATG CA^TATG
1278 CATATG
PflMI 190 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1029 CCAGATTTTGG
SfaNI 1392 GATGC GCATC(5/9)
26 GCATC
SspI 1149 AATATT AAT^ATT
1687 AATATT
TaqI 207 TCGA T^CGA
1641 TCGA
TseI 631 GCAGC G^C[AT]GC
685 GCAGC
Tsp45I 13 GTCAC ^GT[GC]AC
75 GTCAC
TthHB8I 207 TCGA T^CGA
1641 TCGA
Van91I 190 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1029 CCAGATTTTGG
XmnI 719 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
895 GAAATTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
DsaI EcoRII EcoT14I EcoT22I FokI
HaeIII HinfI HphI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI Sau3AI
ScrFI StyI TfiI Tru9I Tsp509I
TspRI XhoII