Restriction map for
pFN21AE1482
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbeI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsiWI Bsp1407I
BspHI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FbaI FseI HindIII HpaI KasI
KpnI MamI MfeI MluI MseI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SexAI SfoI SfuI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 14 TCCGGA T^CCGGA
AcsI 2419 GAATTC [GA]^AATT[CT]
AfaI 906 GTAC GT^AC
AflIII 1835 ACATGT A^C[GA][CT]GT
AgeI 1513 ACCGGT A^CCGGT
AocI 492 CCTGAGG CC^T[ACGT]AGG
ApoI 2419 GAATTC [GA]^AATT[CT]
BamHI 1500 GGATCC G^GATCC
BbvCI 412 CCTCAGC CCTCAGC(-5/-2)
BglII 1304 AGATCT A^GATCT
BseAI 14 TCCGGA T^CCGGA
BsiEI 1276 CGACCG CG[GA][CT]^CG
BsmI 1468 GCATTC GAATGC(1/-1)
BspDI 1546 ATCGAT AT^CGAT
BspEI 14 TCCGGA T^CCGGA
BspLU11I 1835 ACATGT A^CATGT
BstEII 1744 GGTTACC G^GT[ACGT]ACC
BstPI 1744 GGTTACC G^GT[ACGT]ACC
Bsu36I 492 CCTGAGG CC^T[ACGT]AGG
ClaI 1546 ATCGAT AT^CGAT
DrdI 490 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 492 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1744 GGTTACC G^GT[ACGT]ACC
EcoRI 2419 GAATTC G^AATTC
EcoT22I 1466 ATGCAT ATGCA^T
KspI 570 CCGCGG CCGC^GG
MroI 14 TCCGGA T^CCGGA
NaeI 258 GCCGGC GCC^GGC
NgoMIV 258 GCCGGC G^CCGGC
NsiI 1466 ATGCAT ATGCA^T
PinAI 1513 ACCGGT A^CCGGT
RsaI 906 GTAC GT^AC
SacII 570 CCGCGG CCGC^GG
SgrAI 1512 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1578 CTTGAG C^T[CT][GA]AG
Tsp509I 2420 AATT ^AATT
XcmI 1668 CCACGTGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 453 GACGCC G[GA]^CG[CT]C
1624 GGCGTC
Alw44I 1630 GTGCAC G^TGCAC
1672 GTGCAC
ApaLI 1630 GTGCAC G^TGCAC
1672 GTGCAC
Asp700I 186 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2427 GAACAAGTTC
AviII 284 TGCGCA TGC^GCA
2267 TGCGCA
BbrPI 642 CACGTG CAC^GTG
1669 CACGTG
BsaAI 642 CACGTG [CT]AC^GT[GA]
1669 CACGTG
BsaHI 453 GACGCC G[GA]^CG[CT]C
1624 GGCGTC
BsaI 113 GAGACC GGTCTC(1/5)
980 GAGACC
BsaWI 14 TCCGGA [AT]^CCGG[AT]
1513 ACCGGT
BsrBI 1163 CCGCTC CCGCTC(-3/-3)
823 GAGCGG
BsrDI 582 CATTGC GCAATG(2/0)
954 CATTGC
BssSI 100 CTCGTG CACGAG(-5/-1)
1750 CTCGTG
DraIII 619 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2410 CACTGGGTG
FspI 284 TGCGCA TGC^GCA
2267 TGCGCA
Hin1I 453 GACGCC G[GA]^CG[CT]C
1624 GGCGTC
HincII 499 GTCAAC GT[CT]^[GA]AC
1156 GTCAAC
HindII 499 GTCAAC GT[CT]^[GA]AC
1156 GTCAAC
NspI 1067 ACATGC [GA]CATG^[CT]
1835 ACATGT
PmaCI 642 CACGTG CAC^GTG
1669 CACGTG
PmlI 642 CACGTG CAC^GTG
1669 CACGTG
SbfI 201 CCTGCAGG CCTGCA^GG
993 CCTGCAGG
SmaI 272 CCCGGG CCC^GGG
472 CCCGGG
Sse8387I 201 CCTGCAGG CCTGCA^GG
993 CCTGCAGG
StuI 962 AGGCCT AGG^CCT
2274 AGGCCT
Tsp45I 625 GTGAC ^GT[GC]AC
1510 GTCAC
XmaCI 272 CCCGGG C^CCGGG
472 CCCGGG
XmaI 272 CCCGGG C^CCGGG
472 CCCGGG
XmnI 186 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2427 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
StyI TaqI TseI TspRI TthHB8I
XhoII