Restriction map for
pFN21AE3730
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 7 CGCG CG^CG
AcsI 486 GAATTC [GA]^AATT[CT]
AocI 43 CCTGAGG CC^T[ACGT]AGG
ApoI 486 GAATTC [GA]^AATT[CT]
Asp700I 215 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 3 GGACC G^G[AT]CC
BbsI 291 GAAGAC GAAGAC(2/6)
BbvCI 329 CCTCAGC CCTCAGC(-5/-2)
BpuAI 291 GAAGAC GAAGAC(2/6)
BsaI 85 GAGACC GGTCTC(1/5)
BseRI 100 GAGGAG GAGGAG(10/8)
BsmFI 40 GTCCC GGGAC(10/14)
BsrDI 246 CATTGC GCAATG(2/0)
BsrI 37 CCAGT ACTGG(1/-1)
BssKI 312 CCAGG ^CC[ACGT]GG
BstNI 312 CCAGG CC^[AT]GG
BstUI 7 CGCG CG^CG
BstXI 365 CCAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 43 CCTGAGG CC^T[ACGT]AGG
Cfr13I 3 GGACC G^G[ACGT]CC
DraI 400 TTTAAA TTT^AAA
Eco81I 43 CCTGAGG CC^T[ACGT]AGG
EcoNI 43 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 486 GAATTC G^AATTC
EcoRII 312 CCAGG ^CC[AT]GG
HaeIII 327 GGCC GG^CC
KspI 6 CCGCGG CCGC^GG
MaeII 266 ACGT A^CGT
MaeIII 268 GTGAC ^GT[ACGT]AC
MvaI 312 CCAGG CC^[AT]GG
MvnI 7 CGCG CG^CG
PflMI 251 CCAAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 153 GACTC GAGTC(4/5)
PvuII 332 CAGCTG CAG^CTG
SacII 6 CCGCGG CCGC^GG
SapI 109 GAAGAGC GCTCTTC(1/4)
Sau96I 3 GGACC G^G[ACGT]CC
ScrFI 312 CCAGG CC^[ACGT]GG
SfaNI 186 GCATC GCATC(5/9)
SfcI 226 CTACAG C^T[GA][CT]AG
SmlI 161 CTCAAG C^T[CT][GA]AG
StuI 326 AGGCCT AGG^CCT
TaqI 422 TCGA T^CGA
TfiI 215 GAATC G^A[AT]TC
Tsp45I 268 GTGAC ^GT[GC]AC
TspRI 75 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 422 TCGA T^CGA
Van91I 251 CCAAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 215 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 38 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
151 CAGACTCTG
AspI 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
153 GACTCTGTC
BfaI 190 CTAG C^TAG
503 CTAG
BsmAI 85 GAGAC GTCTC(1/5)
159 GTCTC
BstAPI 15 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
274 GCAACATCTGC
Cac8I 11 GCGAGC GC[ACGT]^[ACGT]GC
456 GCCTGC
DpnII 72 GATC ^GATC
198 GATC
DsaI 6 CCGCGG C^C[GA][CT]GG
515 CCACGG
HinfI 153 GACTC G^A[ACGT]TC
215 GAATC
HphI 466 GGTGA GGTGA(8/7)
362 TCACC
MaeI 190 CTAG C^TAG
503 CTAG
MboI 72 GATC ^GATC
198 GATC
MseI 401 TTAA T^TAA
430 TTAA
MspA1I 6 CCGCGG C[AC]G^C[GT]G
332 CAGCTG
NdeII 72 GATC ^GATC
198 GATC
NlaIV 448 GGAACC GG[ACGT]^[ACGT]CC
519 GGAGCC
PflFI 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
153 GACTCTGTC
Sau3AI 72 GATC ^GATC
198 GATC
Tru9I 401 TTAA T^TAA
430 TTAA
Tsp509I 220 AATT ^AATT
487 AATT
Tth111I 34 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
153 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI DdeI EarI Fnu4HI ItaI
Ksp632I MboII MnlI MwoI TseI