Restriction map for
pFN21AE6327
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglI BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tsp509I Tth111I TthHB8I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 9 GGTACC G^GTACC
AlwNI 365 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 9 GGTACC G^GTACC
AspHI 715 GAGCTC G[AT]GC[AT]^C
AviII 137 TGCGCA TGC^GCA
BalI 92 TGGCCA TGG^CCA
BcgI 798 CGAGATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 599 TGATCA T^GATCA
BcnI 6 CCGGG CC^[GC]GG
BfaI 79 CTAG C^TAG
BlpI 808 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 808 GCTGAGC GC^T[ACGT]AGC
BsaAI 739 TACGTG [CT]AC^GT[GA]
BseRI 64 CTCCTC GAGGAG(10/8)
BsgI 590 GTGCAG GTGCAG(16/14)
BsiHKAI 715 GAGCTC G[AT]GC[AT]^C
BsmAI 82 GAGAC GTCTC(1/5)
BsmBI 82 GAGACG CGTCTC(1/5)
BspHI 719 TCATGA T^CATGA
BspMI 586 GCAGGT ACCTGC(4/8)
BsrFI 150 GCCGGT [GA]^CCGG[CT]
BstXI 218 CCACCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 808 GCTGAGC GC^T[ACGT]AGC
Cfr10I 150 GCCGGT [GA]^CCGG[CT]
EagI 29 CGGCCG C^GGCCG
EclXI 29 CGGCCG C^GGCCG
Eco52I 29 CGGCCG C^GGCCG
EcoT14I 423 CCTTGG C^C[AT][AT]GG
FbaI 599 TGATCA T^GATCA
FspI 137 TGCGCA TGC^GCA
KpnI 9 GGTACC GGTAC^C
KspI 246 CCGCGG CCGC^GG
MaeI 79 CTAG C^TAG
MaeII 740 ACGT A^CGT
MluNI 92 TGGCCA TGG^CCA
MscI 92 TGGCCA TGG^CCA
NciI 6 CCGGG CC^[GC]GG
PleI 769 GAGTC GAGTC(4/5)
PvuII 359 CAGCTG CAG^CTG
RcaI 719 TCATGA T^CATGA
SacI 715 GAGCTC GAGCT^C
SacII 246 CCGCGG CCGC^GG
StuI 440 AGGCCT AGG^CCT
StyI 423 CCTTGG C^C[AT][AT]GG
XbaI 78 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 27 CGCG CG^CG
247 CGCG
BbsI 855 GAAGAC GAAGAC(2/6)
892 GAAGAC
BglII 458 AGATCT A^GATCT
800 AGATCT
BpuAI 855 GAAGAC GAAGAC(2/6)
892 GAAGAC
BsiEI 29 CGGCCG CG[GA][CT]^CG
260 CGGTCG
BsmFI 110 GTCCC GGGAC(10/14)
265 GTCCC
BsrDI 375 CATTGC GCAATG(2/0)
305 GCAATG
BsrI 695 ACTGG ACTGG(1/-1)
705 ACTGG
BstEII 153 GGTGACC G^GT[ACGT]ACC
228 GGTCACC
BstPI 153 GGTGACC G^GT[ACGT]ACC
228 GGTCACC
BstUI 27 CGCG CG^CG
247 CGCG
BstYI 458 AGATCT [GA]^GATC[CT]
800 AGATCT
CfoI 26 GCGC GCG^C
138 GCGC
DdeI 22 CTGAG C^T[ACGT]AG
809 CTGAG
EaeI 29 CGGCCG [CT]^GGCC[GA]
92 TGGCCA
EcoO65I 153 GGTGACC G^GT[ACGT]ACC
228 GGTCACC
FauI 72 CCCGC CCCGC(4/6)
187 CCCGC
HapII 6 CCGG C^CGG
151 CCGG
HhaI 26 GCGC GCG^C
138 GCGC
HinP1I 26 GCGC G^CGC
138 GCGC
HpaII 6 CCGG C^CGG
151 CCGG
MflI 458 AGATCT [GA]^GATC[CT]
800 AGATCT
MseI 482 TTAA T^TAA
827 TTAA
MslI 141 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
664 CACATCAATG
MspI 6 CCGG C^CGG
151 CCGG
MvnI 27 CGCG CG^CG
247 CGCG
PstI 200 CTGCAG CTGCA^G
362 CTGCAG
TfiI 745 GATTC G^A[AT]TC
922 GATTC
Tru9I 482 TTAA T^TAA
827 TTAA
XhoII 458 AGATCT [GA]^GATC[CT]
800 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DpnII
DsaI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI