Restriction map for
pFN21AE6432
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BciVI BfrI BglI BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 269 ACGTGT A^C[GA][CT]GT
AspI 1498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 926 TGGCCA TGG^CCA
BbrPI 268 CACGTG CAC^GTG
BbvCI 1570 GCTGAGG CCTCAGC(-5/-2)
BcgI 1358 GCACTGCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 558 TGATCA T^GATCA
BfaI 1664 CTAG C^TAG
BglII 1166 AGATCT A^GATCT
BlpI 1258 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1258 GCTGAGC GC^T[ACGT]AGC
BsaAI 268 CACGTG [CT]AC^GT[GA]
BsiEI 422 CGATCG CG[GA][CT]^CG
BsmI 633 GCATTC GAATGC(1/-1)
BsrDI 1415 GCAATG GCAATG(2/0)
CelII 1258 GCTGAGC GC^T[ACGT]AGC
EarI 1162 GAAGAG CTCTTC(1/4)
EcoNI 1566 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 558 TGATCA T^GATCA
HgaI 45 GACGC GACGC(5/10)
Ksp632I 1162 GAAGAG CTCTTC(1/4)
MaeI 1664 CTAG C^TAG
MaeII 269 ACGT A^CGT
MluNI 926 TGGCCA TGG^CCA
MscI 926 TGGCCA TGG^CCA
NaeI 1263 GCCGGC GCC^GGC
NcoI 84 CCATGG C^CATGG
NgoMIV 1263 GCCGGC G^CCGGC
NspI 1433 ACATGC [GA]CATG^[CT]
PflFI 1498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1072 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 268 CACGTG CAC^GTG
PmlI 268 CACGTG CAC^GTG
PpuMI 953 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 422 CGATCG CGAT^CG
SacI 831 GAGCTC GAGCT^C
SbfI 1011 CCTGCAGG CCTGCA^GG
SmaI 215 CCCGGG CCC^GGG
Sse8387I 1011 CCTGCAGG CCTGCA^GG
TfiI 1094 GAATC G^A[AT]TC
Tsp509I 823 AATT ^AATT
Tth111I 1498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1072 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 215 CCCGGG C^CCGGG
XmaI 215 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 1302 GTAC GT^AC
1596 GTAC
AlwI 1209 GATCC GGATC(4/5)
498 GGATC
BbsI 396 GAAGAC GAAGAC(2/6)
208 GTCTTC
BpmI 693 CTCCAG CTGGAG(16/14)
570 CTGGAG
BpuAI 396 GAAGAC GAAGAC(2/6)
208 GTCTTC
BspMI 1010 ACCTGC ACCTGC(4/8)
1584 GCAGGT
BsrFI 1263 GCCGGC [GA]^CCGG[CT]
1285 ACCGGC
BstXI 41 CCATGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1226 CCACCAATGTGG
CfoI 174 GCGC GCG^C
1467 GCGC
Cfr10I 1263 GCCGGC [GA]^CCGG[CT]
1285 ACCGGC
DsaI 84 CCATGG C^C[GA][CT]GG
1579 CCACGG
HhaI 174 GCGC GCG^C
1467 GCGC
HinP1I 174 GCGC G^CGC
1467 GCGC
MaeIII 354 GTCAC ^GT[ACGT]AC
367 GTCAC
PstI 1012 CTGCAG CTGCA^G
1487 CTGCAG
RsaI 1302 GTAC GT^AC
1596 GTAC
ScaI 1301 AGTACT AGT^ACT
1595 AGTACT
SfcI 1012 CTGCAG C^T[GA][CT]AG
1487 CTGCAG
Tsp45I 354 GTCAC ^GT[GC]AC
367 GTCAC
XcmI 416 CCATCACGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1556 CCAAGTGTGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI Alw44I AlwNI ApaLI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TseI
TspRI TthHB8I XhoII