Restriction map for
pFN21AE3639
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanII BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BspHI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII Cfr10I CpoI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoT14I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 30 CGCG CG^CG
AcyI 1057 GGCGCC G[GA]^CG[CT]C
AflIII 526 ACATGT A^C[GA][CT]GT
Alw44I 944 GTGCAC G^TGCAC
AocI 1044 CCTGAGG CC^T[ACGT]AGG
ApaLI 944 GTGCAC G^TGCAC
AspHI 944 GTGCAC G[AT]GC[AT]^C
BamHI 1110 GGATCC G^GATCC
BbeI 1057 GGCGCC GGCGC^C
BbsI 1001 GAAGAC GAAGAC(2/6)
BfaI 802 CTAG C^TAG
BpuAI 1001 GAAGAC GAAGAC(2/6)
BsaHI 1057 GGCGCC G[GA]^CG[CT]C
BsaI 905 GAGACC GGTCTC(1/5)
BsiHKAI 944 GTGCAC G[AT]GC[AT]^C
BsmAI 905 GAGAC GTCTC(1/5)
BsmI 227 GAATGC GAATGC(1/-1)
BspDI 118 ATCGAT AT^CGAT
BspLU11I 526 ACATGT A^CATGT
BsrBI 1102 GAGCGG CCGCTC(-3/-3)
BstEII 895 GGTTACC G^GT[ACGT]ACC
BstPI 895 GGTTACC G^GT[ACGT]ACC
BstUI 30 CGCG CG^CG
Bsu36I 1044 CCTGAGG CC^T[ACGT]AGG
ClaI 118 ATCGAT AT^CGAT
DraI 520 TTTAAA TTT^AAA
DraII 760 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 612 CCACGG C^C[GA][CT]GG
Eco81I 1044 CCTGAGG CC^T[ACGT]AGG
EcoO109I 760 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 895 GGTTACC G^GT[ACGT]ACC
EcoRV 160 GATATC GAT^ATC
EcoT22I 861 ATGCAT ATGCA^T
HgaI 584 GACGC GACGC(5/10)
Hin1I 1057 GGCGCC G[GA]^CG[CT]C
HincII 530 GTCAAC GT[CT]^[GA]AC
HindII 530 GTCAAC GT[CT]^[GA]AC
HphI 126 TCACC GGTGA(8/7)
KasI 1057 GGCGCC G^GCGCC
MaeI 802 CTAG C^TAG
MvnI 30 CGCG CG^CG
NarI 1057 GGCGCC GG^CGCC
NsiI 861 ATGCAT ATGCA^T
PflMI 415 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 675 GACTC GAGTC(4/5)
PpuMI 760 GGGACCT [GA]G^G[AT]CC[CT]
PstI 278 CTGCAG CTGCA^G
ScaI 138 AGTACT AGT^ACT
SfcI 278 CTGCAG C^T[GA][CT]AG
SfoI 1057 GGCGCC GGC^GCC
Van91I 415 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 616 GGACC G^G[AT]CC
761 GGACC
BcnI 5 CCCGG CC^[GC]GG
83 CCGGG
BmyI 543 GTGCCC G[AGT]GC[ACT]^C
944 GTGCAC
BpmI 794 CTCCAG CTGGAG(16/14)
42 CTGGAG
BsmFI 760 GGGAC GGGAC(10/14)
361 GTCCC
Bsp1286I 543 GTGCCC G[AGT]GC[ACT]^C
944 GTGCAC
BspMI 745 ACCTGC ACCTGC(4/8)
955 ACCTGC
BstYI 345 AGATCC [GA]^GATC[CT]
1110 GGATCC
EcoRI 304 GAATTC G^AATTC
471 GAATTC
HaeII 13 AGCGCC [GA]GCGC^[CT]
1057 GGCGCC
HapII 6 CCGG C^CGG
83 CCGG
HpaII 6 CCGG C^CGG
83 CCGG
MflI 345 AGATCC [GA]^GATC[CT]
1110 GGATCC
MspI 6 CCGG C^CGG
83 CCGG
NciI 5 CCCGG CC^[GC]GG
83 CCGGG
NspI 526 ACATGT [GA]CATG^[CT]
1038 ACATGC
TaqI 119 TCGA T^CGA
846 TCGA
TfiI 704 GATTC G^A[AT]TC
1120 GAATC
Tsp45I 673 GTGAC ^GT[GC]AC
722 GTGAC
TthHB8I 119 TCGA T^CGA
846 TCGA
XhoII 345 AGATCC [GA]^GATC[CT]
1110 GGATCC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanI BbvI BsaJI BseRI
BsiYI BslI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp509I TspRI