Restriction map for
pFN21AE5505
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MaeI MaeII MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 709 GTATAC GT^[AC][GT]AC
AlwI 78 GATCC GGATC(4/5)
ApaI 345 GGGCCC GGGCC^C
Asp700I 1149 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2 TGGCCA TGG^CCA
BanII 345 GGGCCC G[GA]GC[CT]^C
BglII 640 AGATCT A^GATCT
BmyI 345 GGGCCC G[AGT]GC[ACT]^C
BsaBI 1005 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 23 GTCCC GGGAC(10/14)
Bsp1286I 345 GGGCCC G[AGT]GC[ACT]^C
BspHI 173 TCATGA T^CATGA
BsrDI 378 CATTGC GCAATG(2/0)
Bst1107I 709 GTATAC GTA^TAC
BstAPI 365 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 640 AGATCT [GA]^GATC[CT]
BstZ17I 709 GTATAC GTA^TAC
Cac8I 109 GCTCGC GC[ACGT]^[ACGT]GC
DraIII 987 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
HapII 938 CCGG C^CGG
HgaI 966 GACGC GACGC(5/10)
HpaII 938 CCGG C^CGG
HphI 182 TCACC GGTGA(8/7)
MaeIII 396 GTTAC ^GT[ACGT]AC
MamI 1005 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 640 AGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 1107 CAGCGG C[AC]G^C[GT]G
MspI 938 CCGG C^CGG
NdeI 660 CATATG CA^TATG
PpuMI 1127 AGGACCC [GA]G^G[AT]CC[CT]
PstI 372 CTGCAG CTGCA^G
RcaI 173 TCATGA T^CATGA
SfcI 372 CTGCAG C^T[GA][CT]AG
TaqI 1075 TCGA T^CGA
TfiI 232 GATTC G^A[AT]TC
TthHB8I 1075 TCGA T^CGA
XhoII 640 AGATCT [GA]^GATC[CT]
XmnI 1149 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 977 GCGG CCGC(-3/-1)
1109 GCGG
AfaI 738 GTAC GT^AC
1096 GTAC
AlwNI 366 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
950 CAGCAACTG
AvaII 656 GGTCC G^G[AT]CC
1128 GGACC
BsrI 399 ACTGG ACTGG(1/-1)
1043 CCAGT
BssKI 83 CCAGG ^CC[ACGT]GG
794 CCTGG
BstNI 83 CCAGG CC^[AT]GG
794 CCTGG
DpnII 78 GATC ^GATC
641 GATC
EcoRII 83 CCAGG ^CC[AT]GG
794 CCTGG
HpaI 745 GTTAAC GTT^AAC
1174 GTTAAC
MboI 78 GATC ^GATC
641 GATC
MvaI 83 CCAGG CC^[AT]GG
794 CCTGG
NdeII 78 GATC ^GATC
641 GATC
RsaI 738 GTAC GT^AC
1096 GTAC
Sau3AI 78 GATC ^GATC
641 GATC
ScrFI 83 CCAGG CC^[ACGT]GG
794 CCTGG
SfaNI 404 GATGC GCATC(5/9)
1060 GCATC
XcmI 185 CCATGGTAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1043 CCAGTTTTCTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI BbsI BbvI BpuAI BsaJI
BsiYI BslI BsmAI BstF5I Cfr13I
DdeI DraII DsaI EarI EcoO109I
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI ItaI Ksp632I MboII
MnlI MseI MslI MwoI NcoI
NlaIII NlaIV PleI Sau96I StyI
Tru9I TseI Tsp509I TspRI