Restriction map for
pFN21AE5504
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# NO CUT ENZYMES ( 128 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SspBI SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 1123 GACGTC GACGT^C
AlwNI 1075 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 589 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1239 TGCGCA TGC^GCA
BamHI 1112 GGATCC G^GATCC
BciVI 846 GGATAC GTATCC(6/5)
BfaI 380 CTAG C^TAG
BglI 1095 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 78 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1066 TCCGGT [AT]^CCGG[AT]
BsgI 226 GTGCAG GTGCAG(16/14)
BsiEI 1155 CGACCG CG[GA][CT]^CG
BsrBI 1214 GAGCGG CCGCTC(-3/-3)
BstAPI 21 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 1158 CCGCGG C^C[GA][CT]GG
EaeI 1145 TGGCCG [CT]^GGCC[GA]
EcoT14I 824 CCAAGG C^C[AT][AT]GG
FspI 1239 TGCGCA TGC^GCA
HaeII 1177 AGCGCC [GA]GCGC^[CT]
KspI 1158 CCGCGG CCGC^GG
MaeI 380 CTAG C^TAG
MamI 78 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1004 TTAA T^TAA
NspI 897 GCATGC [GA]CATG^[CT]
PstI 1097 CTGCAG CTGCA^G
SacI 154 GAGCTC GAGCT^C
SacII 1158 CCGCGG CCGC^GG
SbfI 1096 CCTGCAGG CCTGCA^GG
SphI 897 GCATGC GCATG^C
Sse8387I 1096 CCTGCAGG CCTGCA^GG
StyI 824 CCAAGG C^C[AT][AT]GG
Tru9I 1004 TTAA T^TAA
Tsp509I 243 AATT ^AATT
XcmI 575 CCATTCTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 589 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 1048 GGCGTC G[GA]^CG[CT]C
1123 GACGTC
AspHI 154 GAGCTC G[AT]GC[AT]^C
948 GTGCTC
AvaI 1181 CCCGGG C^[CT]CG[GA]G
1255 CCCGGG
BsaHI 1048 GGCGTC G[GA]^CG[CT]C
1123 GACGTC
BsiHKAI 154 GAGCTC G[AT]GC[AT]^C
948 GTGCTC
BsoBI 1181 CCCGGG C^[CT]CG[GA]G
1255 CCCGGG
BssHII 1195 GCGCGC G^CGCGC
1197 GCGCGC
BstXI 390 CCAGGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCATTTTGATGG
EcoNI 447 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
682 CCTGATGGAGG
HgaI 110 GACGC GACGC(5/10)
1049 GCGTC
Hin1I 1048 GGCGTC G[GA]^CG[CT]C
1123 GACGTC
HphI 1120 GGTGA GGTGA(8/7)
740 TCACC
MslI 544 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
741 CACCTTCGTG
MspA1I 13 CCGCTG C[AC]G^C[GT]G
1158 CCGCGG
PleI 813 GACTC GAGTC(4/5)
98 GAGTC
SapI 676 GAAGAGC GCTCTTC(1/4)
1170 GAAGAGC
SfcI 216 CTACAG C^T[GA][CT]AG
1097 CTGCAG
SmaI 1181 CCCGGG CCC^GGG
1255 CCCGGG
Tsp45I 754 GTCAC ^GT[GC]AC
1121 GTGAC
XmaCI 1181 CCCGGG C^CCGGG
1255 CCCGGG
XmaI 1181 CCCGGG C^CCGGG
1255 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
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AccII AciI AfaI AluI AlwI
ApaI AvaII BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI TseI
TspRI TthHB8I Van91I XhoII