Restriction map for
pFN21AE1337
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BglI BglII
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MflI
MluI MroI MslI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 295 GTAC GT^AC
AflII 70 CTTAAG C^TTAAG
AluI 209 AGCT AG^CT
AlwI 150 GGATC GGATC(4/5)
AocI 361 CCTGAGG CC^T[ACGT]AGG
AvrII 225 CCTAGG C^CTAGG
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
BbvI 57 GCAGC GCAGC(8/12)
BfaI 226 CTAG C^TAG
BfrI 70 CTTAAG C^TTAAG
BlnI 225 CCTAGG C^CTAGG
BseRI 364 GAGGAG GAGGAG(10/8)
BsiYI 283 CCTTGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 283 CCTTGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 329 GAATGC GAATGC(1/-1)
BsrI 297 ACTGG ACTGG(1/-1)
BssKI 42 CCAGG ^CC[ACGT]GG
BstBI 449 TTCGAA TT^CGAA
BstNI 42 CCAGG CC^[AT]GG
Bsu36I 361 CCTGAGG CC^T[ACGT]AGG
Cac8I 437 GCTGGC GC[ACGT]^[ACGT]GC
Eco81I 361 CCTGAGG CC^T[ACGT]AGG
EcoRII 42 CCAGG ^CC[AT]GG
EcoT14I 225 CCTAGG C^C[AT][AT]GG
Fnu4HI 57 GCAGC GC^[ACGT]GC
ItaI 57 GCAGC GC^[ACGT]GC
MaeI 226 CTAG C^TAG
MaeII 342 ACGT A^CGT
MvaI 42 CCAGG CC^[AT]GG
NspV 449 TTCGAA TT^CGAA
PleI 33 GAGTC GAGTC(4/5)
RsaI 295 GTAC GT^AC
ScaI 294 AGTACT AGT^ACT
ScrFI 42 CCAGG CC^[ACGT]GG
SfuI 449 TTCGAA TT^CGAA
SmlI 70 CTTAAG C^T[CT][GA]AG
SspI 111 AATATT AAT^ATT
StyI 225 CCTAGG C^C[AT][AT]GG
TaqI 450 TCGA T^CGA
TfiI 230 GAATC G^A[AT]TC
TseI 57 GCAGC G^C[AT]GC
TthHB8I 450 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AvaII 45 GGACC G^G[AT]CC
222 GGTCC
BalI 251 TGGCCA TGG^CCA
351 TGGCCA
BpmI 200 CTCCAG CTGGAG(16/14)
298 CTGGAG
BsaJI 41 CCCAGG C^C[ACGT][ACGT]GG
225 CCTAGG
DpnII 151 GATC ^GATC
462 GATC
DraII 44 AGGACCC [GA]G^G[ACGT]CC[CT]
221 AGGTCCT
EaeI 251 TGGCCA [CT]^GGCC[GA]
351 TGGCCA
EcoO109I 44 AGGACCC [GA]G^G[ACGT]CC[CT]
221 AGGTCCT
HinfI 33 GAGTC G^A[ACGT]TC
230 GAATC
MboI 151 GATC ^GATC
462 GATC
MluNI 251 TGGCCA TGG^CCA
351 TGGCCA
MscI 251 TGGCCA TGG^CCA
351 TGGCCA
NdeII 151 GATC ^GATC
462 GATC
PpuMI 44 AGGACCC [GA]G^G[AT]CC[CT]
221 AGGTCCT
Sau3AI 151 GATC ^GATC
462 GATC
Tsp509I 125 AATT ^AATT
310 AATT
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
Cfr13I DdeI HaeIII MboII MnlI
MseI NlaIV Sau96I Tru9I TspRI