Restriction map for
pFN21AE4492
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BalI BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BpuAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI TfiI
Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 477 GTCTAC GT^[AC][GT]AC
AccII 1039 CGCG CG^CG
AcsI 133 AAATTC [GA]^AATT[CT]
AflIII 481 ACACGT A^C[GA][CT]GT
Alw44I 582 GTGCAC G^TGCAC
AlwNI 1353 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 582 GTGCAC G^TGCAC
ApoI 133 AAATTC [GA]^AATT[CT]
AspI 775 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1047 CCTAGG C^CTAGG
BcgI 1011 GCACCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1048 CTAG C^TAG
BlnI 1047 CCTAGG C^CTAGG
BlpI 1161 GCTCAGC GC^T[ACGT]AGC
BpmI 355 CTCCAG CTGGAG(16/14)
Bpu1102I 1161 GCTCAGC GC^T[ACGT]AGC
BsaAI 482 CACGTA [CT]AC^GT[GA]
BsaI 1193 GGTCTC GGTCTC(1/5)
BsaWI 839 ACCGGA [AT]^CCGG[AT]
BsmBI 187 GAGACG CGTCTC(1/5)
BsmI 1302 GAATGC GAATGC(1/-1)
Bsp1407I 308 TGTACA T^GTACA
BspHI 575 TCATGA T^CATGA
BsrBI 790 GAGCGG CCGCTC(-3/-3)
BsrGI 308 TGTACA T^GTACA
BstUI 1039 CGCG CG^CG
BstXI 357 CCAGTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1161 GCTCAGC GC^T[ACGT]AGC
DrdI 1264 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 315 CCGTGG C^C[GA][CT]GG
FauI 744 GCGGG CCCGC(4/6)
MaeI 1048 CTAG C^TAG
MvnI 1039 CGCG CG^CG
NspI 168 ACATGC [GA]CATG^[CT]
PaeR7I 175 CTCGAG C^TCGAG
PflFI 775 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 149 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 320 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 575 TCATGA T^CATGA
SexAI 776 ACCAGGT A^CC[AT]GGT
SmaI 878 CCCGGG CCC^GGG
SspBI 308 TGTACA T^GTACA
StuI 372 AGGCCT AGG^CCT
TaqI 176 TCGA T^CGA
Tsp509I 134 AATT ^AATT
Tth111I 775 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 176 TCGA T^CGA
Van91I 149 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 153 CCACTGGCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 175 CTCGAG C^TCGAG
XmaCI 878 CCCGGG C^CCGGG
XmaI 878 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 325 CCTAAGG CC^T[ACGT]AGG
1267 CCTGAGG
AspHI 582 GTGCAC G[AT]GC[AT]^C
1009 GAGCAC
BsiHKAI 582 GTGCAC G[AT]GC[AT]^C
1009 GAGCAC
BspMI 92 ACCTGC ACCTGC(4/8)
1200 ACCTGC
BsrFI 604 ACCGGC [GA]^CCGG[CT]
1190 GCCGGT
Bsu36I 325 CCTAAGG CC^T[ACGT]AGG
1267 CCTGAGG
Cfr10I 604 ACCGGC [GA]^CCGG[CT]
1190 GCCGGT
EarI 505 GAAGAG CTCTTC(1/4)
593 GAAGAG
Eco81I 325 CCTAAGG CC^T[ACGT]AGG
1267 CCTGAGG
EcoT14I 35 CCTTGG C^C[AT][AT]GG
1047 CCTAGG
EcoT22I 1222 ATGCAT ATGCA^T
1304 ATGCAT
HaeII 561 GGCGCT [GA]GCGC^[CT]
674 AGCGCC
HinfI 475 GAGTC G^A[ACGT]TC
1279 GACTC
Ksp632I 505 GAAGAG CTCTTC(1/4)
593 GAAGAG
MaeII 112 ACGT A^CGT
483 ACGT
NsiI 1222 ATGCAT ATGCA^T
1304 ATGCAT
PleI 1279 GACTC GAGTC(4/5)
475 GAGTC
PstI 805 CTGCAG CTGCA^G
1202 CTGCAG
SbfI 804 CCTGCAGG CCTGCA^GG
1201 CCTGCAGG
SfcI 805 CTGCAG C^T[GA][CT]AG
1202 CTGCAG
Sse8387I 804 CCTGCAGG CCTGCA^GG
1201 CCTGCAGG
StyI 35 CCTTGG C^C[AT][AT]GG
1047 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TseI Tsp45I TspRI
XhoII