Restriction map for
pFN21AE3587
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspLU11I BsrBI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HinfI
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwNI 124 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 607 CCTCAGG CC^T[ACGT]AGG
Asp700I 483 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 612 GGTGCC G^G[CT][GA]CC
BbsI 586 GAAGAC GAAGAC(2/6)
BglI 275 GCCGCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 586 GAAGAC GAAGAC(2/6)
BsaAI 256 TACGTG [CT]AC^GT[GA]
BsiEI 334 CGGCCG CG[GA][CT]^CG
Bsp1407I 263 TGTACA T^GTACA
BspHI 695 TCATGA T^CATGA
BsrGI 263 TGTACA T^GTACA
BstAPI 252 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 479 GGATG GGATG(2/0)
Bsu36I 607 CCTCAGG CC^T[ACGT]AGG
DpnII 573 GATC ^GATC
EagI 334 CGGCCG C^GGCCG
EarI 627 GAAGAG CTCTTC(1/4)
EclXI 334 CGGCCG C^GGCCG
Eco52I 334 CGGCCG C^GGCCG
Eco81I 607 CCTCAGG CC^T[ACGT]AGG
FokI 479 GGATG GGATG(9/13)
HindIII 619 AAGCTT A^AGCTT
Ksp632I 627 GAAGAG CTCTTC(1/4)
MboI 573 GATC ^GATC
NaeI 418 GCCGGC GCC^GGC
NcoI 554 CCATGG C^CATGG
NdeII 573 GATC ^GATC
NgoMIV 418 GCCGGC G^CCGGC
NspI 96 GCATGC [GA]CATG^[CT]
RcaI 695 TCATGA T^CATGA
SapI 627 GAAGAGC GCTCTTC(1/4)
Sau3AI 573 GATC ^GATC
ScaI 428 AGTACT AGT^ACT
SfcI 384 CTACAG C^T[GA][CT]AG
SmaI 217 CCCGGG CCC^GGG
SmlI 622 CTTGAG C^T[CT][GA]AG
SphI 96 GCATGC GCATG^C
SspBI 263 TGTACA T^GTACA
TspRI 463 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 217 CCCGGG C^CCGGG
XmaI 217 CCCGGG C^CCGGG
XmnI 483 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Aor51HI 317 AGCGCT AGC^GCT
380 AGCGCT
AvaI 204 CTCGGG C^[CT]CG[GA]G
217 CCCGGG
BsgI 460 CTGCAC GTGCAG(16/14)
151 GTGCAG
BsoBI 204 CTCGGG C^[CT]CG[GA]G
217 CCCGGG
BssSI 424 CACGAG CACGAG(-5/-1)
34 CTCGTG
BstNI 306 CCAGG CC^[AT]GG
669 CCTGG
DdeI 475 CTGAG C^T[ACGT]AG
608 CTCAG
DsaI 554 CCATGG C^C[GA][CT]GG
674 CCGTGG
Eco47III 317 AGCGCT AGC^GCT
380 AGCGCT
EcoRII 306 CCAGG ^CC[AT]GG
669 CCTGG
EcoT14I 66 CCAAGG C^C[AT][AT]GG
554 CCATGG
MvaI 306 CCAGG CC^[AT]GG
669 CCTGG
SfaNI 632 GCATC GCATC(5/9)
680 GCATC
StyI 66 CCAAGG C^C[AT][AT]GG
554 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI Cac8I CfoI
Cfr10I Cfr13I DraII EaeI EcoO109I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MboII MnlI MspA1I MspI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI TseI