Restriction map for
pFN21AE1547
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AgeI AhdI AlwI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BbvCI
BciVI BglI BglII BlnI BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FseI
FspI HgaI HpaI KpnI Ksp632I
KspI MfeI MflI MluI MroI
MspA1I MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SgrAI SnaBI Sse8387I SspBI SspI
SwaI XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 30 GGCGCC G[GA]^CG[CT]C
AflII 1216 CTTAAG C^TTAAG
AflIII 1121 ACACGT A^C[GA][CT]GT
Alw44I 1577 GTGCAC G^TGCAC
ApaI 1253 GGGCCC GGGCC^C
ApaLI 1577 GTGCAC G^TGCAC
AspI 1422 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 3 GGTCC G^G[AT]CC
BalI 248 TGGCCA TGG^CCA
BbeI 30 GGCGCC GGCGC^C
BbsI 1758 GTCTTC GAAGAC(2/6)
BcgI 1677 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 617 TGATCA T^GATCA
BfaI 1327 CTAG C^TAG
BfrI 1216 CTTAAG C^TTAAG
BlpI 453 GCTCAGC GC^T[ACGT]AGC
BpmI 356 CTGGAG CTGGAG(16/14)
Bpu1102I 453 GCTCAGC GC^T[ACGT]AGC
BpuAI 1758 GTCTTC GAAGAC(2/6)
BsaBI 961 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 30 GGCGCC G[GA]^CG[CT]C
BseRI 715 GAGGAG GAGGAG(10/8)
BsmAI 1663 GAGAC GTCTC(1/5)
BspHI 1410 TCATGA T^CATGA
BspMI 1701 ACCTGC ACCTGC(4/8)
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BsrDI 173 CATTGC GCAATG(2/0)
BstAPI 84 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 518 TTCGAA TT^CGAA
BstXI 655 CCAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 453 GCTCAGC GC^T[ACGT]AGC
DsaI 1364 CCGTGG C^C[GA][CT]GG
EcoNI 246 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 617 TGATCA T^GATCA
Hin1I 30 GGCGCC G[GA]^CG[CT]C
HphI 1392 GGTGA GGTGA(8/7)
KasI 30 GGCGCC G^GCGCC
MaeI 1327 CTAG C^TAG
MamI 961 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
NarI 30 GGCGCC GG^CGCC
NspV 518 TTCGAA TT^CGAA
PaeR7I 473 CTCGAG C^TCGAG
PflFI 1422 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 225 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1410 TCATGA T^CATGA
ScaI 1535 AGTACT AGT^ACT
SfoI 30 GGCGCC GGC^GCC
SfuI 518 TTCGAA TT^CGAA
SpeI 1326 ACTAGT A^CTAGT
SphI 1283 GCATGC GCATG^C
Tth111I 1422 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 225 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 473 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 1104 GTAC GT^AC
1536 GTAC
CfoI 31 GCGC GCG^C
430 GCGC
DpnII 280 GATC ^GATC
618 GATC
EaeI 182 TGGCCG [CT]^GGCC[GA]
248 TGGCCA
EcoRV 347 GATATC GAT^ATC
1468 GATATC
EcoT14I 251 CCAAGG C^C[AT][AT]GG
1656 CCAAGG
HaeII 30 GGCGCC [GA]GCGC^[CT]
429 GGCGCT
HhaI 31 GCGC GCG^C
430 GCGC
HinP1I 31 GCGC G^CGC
430 GCGC
HincII 1162 GTCAAC GT[CT]^[GA]AC
1419 GTTGAC
HindII 1162 GTCAAC GT[CT]^[GA]AC
1419 GTTGAC
HindIII 1405 AAGCTT A^AGCTT
1565 AAGCTT
MboI 280 GATC ^GATC
618 GATC
NdeII 280 GATC ^GATC
618 GATC
NspI 1149 ACATGC [GA]CATG^[CT]
1283 GCATGC
PleI 1665 GACTC GAGTC(4/5)
1426 GAGTC
RsaI 1104 GTAC GT^AC
1536 GTAC
SacI 451 GAGCTC GAGCT^C
765 GAGCTC
Sau3AI 280 GATC ^GATC
618 GATC
SfcI 1176 CTACAG C^T[GA][CT]AG
1642 CTACAG
SmaI 110 CCCGGG CCC^GGG
1607 CCCGGG
SmlI 473 CTCGAG C^T[CT][GA]AG
1216 CTTAAG
StuI 254 AGGCCT AGG^CCT
1622 AGGCCT
StyI 251 CCAAGG C^C[AT][AT]GG
1656 CCAAGG
TfiI 738 GAATC G^A[AT]TC
1276 GATTC
Tsp45I 16 GTGAC ^GT[GC]AC
1765 GTCAC
XmaCI 110 CCCGGG C^CCGGG
1607 CCCGGG
XmaI 110 CCCGGG C^CCGGG
1607 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AspHI AvaI BanI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeII
MaeIII MboII MnlI MseI MslI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I