################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspDI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FseI FspI Hin1I KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AocI 169 CCTGAGG CC^T[ACGT]AGG AseI 1078 ATTAAT AT^TAAT AsnI 1078 ATTAAT AT^TAAT Asp700I 2726 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 955 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2824 GGATCC G^GATCC BanI 708 GGTGCC G^G[CT][GA]CC BbsI 317 GTCTTC GAAGAC(2/6) BcnI 26 CCCGG CC^[GC]GG BglI 98 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 317 GTCTTC GAAGAC(2/6) BsaI 2001 GAGACC GGTCTC(1/5) BsaWI 1034 ACCGGA [AT]^CCGG[AT] BsgI 2762 GTGCAG GTGCAG(16/14) BsrDI 2368 CATTGC GCAATG(2/0) Bst1107I 82 GTATAC GTA^TAC BstZ17I 82 GTATAC GTA^TAC Bsu36I 169 CCTGAGG CC^T[ACGT]AGG EaeI 2100 CGGCCA [CT]^GGCC[GA] Eco81I 169 CCTGAGG CC^T[ACGT]AGG EcoRI 607 GAATTC G^AATTC FauI 2470 CCCGC CCCGC(4/6) HaeII 2135 GGCGCT [GA]GCGC^[CT] HgaI 1095 GACGC GACGC(5/10) HincII 1176 GTTAAC GT[CT]^[GA]AC HindII 1176 GTTAAC GT[CT]^[GA]AC HindIII 1568 AAGCTT A^AGCTT HpaI 1176 GTTAAC GTT^AAC MslI 129 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 26 CCCGG CC^[GC]GG NdeI 329 CATATG CA^TATG PaeR7I 1376 CTCGAG C^TCGAG PflFI 955 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1078 ATTAAT AT^TAAT PstI 2441 CTGCAG CTGCA^G SnaBI 2202 TACGTA TAC^GTA SspI 1075 AATATT AAT^ATT Tth111I 955 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2829 CCAGCTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1376 CTCGAG C^TCGAG XmnI 2726 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 82 GTATAC GT^[AC][GT]AC 1324 GTAGAC AlwNI 2528 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG 2765 CAGAGTCTG AvaI 1376 CTCGAG C^[CT]CG[GA]G 2009 CTCGGG BsaAI 1061 TACGTG [CT]AC^GT[GA] 2202 TACGTA BseRI 820 GAGGAG GAGGAG(10/8) 1356 GAGGAG BsmBI 2416 CGTCTC CGTCTC(1/5) 858 GAGACG BsoBI 1376 CTCGAG C^[CT]CG[GA]G 2009 CTCGGG Bsp1407I 600 TGTACA T^GTACA 793 TGTACA BspHI 1277 TCATGA T^CATGA 2111 TCATGA BspMI 2745 ACCTGC ACCTGC(4/8) 2375 GCAGGT BsrGI 600 TGTACA T^GTACA 793 TGTACA DraII 49 GGGTCCC [GA]G^G[ACGT]CC[CT] 2835 GGGACCT EcoO109I 49 GGGTCCC [GA]G^G[ACGT]CC[CT] 2835 GGGACCT EcoRV 1459 GATATC GAT^ATC 2843 GATATC HaeIII 847 GGCC GG^CC 2101 GGCC HapII 27 CCGG C^CGG 1035 CCGG HpaII 27 CCGG C^CGG 1035 CCGG MspA1I 1909 CAGCTG C[AC]G^C[GT]G 2830 CAGCTG MspI 27 CCGG C^CGG 1035 CCGG PpuMI 49 GGGTCCC [GA]G^G[AT]CC[CT] 2835 GGGACCT PvuII 1909 CAGCTG CAG^CTG 2830 CAGCTG RcaI 1277 TCATGA T^CATGA 2111 TCATGA SmlI 1298 CTCAAG C^T[CT][GA]AG 1376 CTCGAG SspBI 600 TGTACA T^GTACA 793 TGTACA TspRI 101 CACTG CA[GC]TG[ACGT][ACGT]^ 490 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AvaII BbvI BclI BfaI BglII BpmI BsaJI BsiYI BslI BsmAI BsmFI BspLU11I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I XhoII