Restriction map for
pFN21AE1573
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AviII AvrII BalI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfrI BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FseI FspI Hin1I KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I StuI SwaI
Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AocI 169 CCTGAGG CC^T[ACGT]AGG
AseI 1078 ATTAAT AT^TAAT
AsnI 1078 ATTAAT AT^TAAT
Asp700I 2726 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 955 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2824 GGATCC G^GATCC
BanI 708 GGTGCC G^G[CT][GA]CC
BbsI 317 GTCTTC GAAGAC(2/6)
BcnI 26 CCCGG CC^[GC]GG
BglI 98 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 317 GTCTTC GAAGAC(2/6)
BsaI 2001 GAGACC GGTCTC(1/5)
BsaWI 1034 ACCGGA [AT]^CCGG[AT]
BsgI 2762 GTGCAG GTGCAG(16/14)
BsrDI 2368 CATTGC GCAATG(2/0)
Bst1107I 82 GTATAC GTA^TAC
BstZ17I 82 GTATAC GTA^TAC
Bsu36I 169 CCTGAGG CC^T[ACGT]AGG
EaeI 2100 CGGCCA [CT]^GGCC[GA]
Eco81I 169 CCTGAGG CC^T[ACGT]AGG
EcoRI 607 GAATTC G^AATTC
FauI 2470 CCCGC CCCGC(4/6)
HaeII 2135 GGCGCT [GA]GCGC^[CT]
HgaI 1095 GACGC GACGC(5/10)
HincII 1176 GTTAAC GT[CT]^[GA]AC
HindII 1176 GTTAAC GT[CT]^[GA]AC
HindIII 1568 AAGCTT A^AGCTT
HpaI 1176 GTTAAC GTT^AAC
MslI 129 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 26 CCCGG CC^[GC]GG
NdeI 329 CATATG CA^TATG
PaeR7I 1376 CTCGAG C^TCGAG
PflFI 955 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1078 ATTAAT AT^TAAT
PstI 2441 CTGCAG CTGCA^G
SnaBI 2202 TACGTA TAC^GTA
SspI 1075 AATATT AAT^ATT
Tth111I 955 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2829 CCAGCTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1376 CTCGAG C^TCGAG
XmnI 2726 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 82 GTATAC GT^[AC][GT]AC
1324 GTAGAC
AlwNI 2528 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
2765 CAGAGTCTG
AvaI 1376 CTCGAG C^[CT]CG[GA]G
2009 CTCGGG
BsaAI 1061 TACGTG [CT]AC^GT[GA]
2202 TACGTA
BseRI 820 GAGGAG GAGGAG(10/8)
1356 GAGGAG
BsmBI 2416 CGTCTC CGTCTC(1/5)
858 GAGACG
BsoBI 1376 CTCGAG C^[CT]CG[GA]G
2009 CTCGGG
Bsp1407I 600 TGTACA T^GTACA
793 TGTACA
BspHI 1277 TCATGA T^CATGA
2111 TCATGA
BspMI 2745 ACCTGC ACCTGC(4/8)
2375 GCAGGT
BsrGI 600 TGTACA T^GTACA
793 TGTACA
DraII 49 GGGTCCC [GA]G^G[ACGT]CC[CT]
2835 GGGACCT
EcoO109I 49 GGGTCCC [GA]G^G[ACGT]CC[CT]
2835 GGGACCT
EcoRV 1459 GATATC GAT^ATC
2843 GATATC
HaeIII 847 GGCC GG^CC
2101 GGCC
HapII 27 CCGG C^CGG
1035 CCGG
HpaII 27 CCGG C^CGG
1035 CCGG
MspA1I 1909 CAGCTG C[AC]G^C[GT]G
2830 CAGCTG
MspI 27 CCGG C^CGG
1035 CCGG
PpuMI 49 GGGTCCC [GA]G^G[AT]CC[CT]
2835 GGGACCT
PvuII 1909 CAGCTG CAG^CTG
2830 CAGCTG
RcaI 1277 TCATGA T^CATGA
2111 TCATGA
SmlI 1298 CTCAAG C^T[CT][GA]AG
1376 CTCGAG
SspBI 600 TGTACA T^GTACA
793 TGTACA
TspRI 101 CACTG CA[GC]TG[ACGT][ACGT]^
490 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BbvI BclI
BfaI BglII BpmI BsaJI BsiYI
BslI BsmAI BsmFI BspLU11I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TthHB8I XhoII