Restriction map for
pFN21AE4456
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HpaI HpaII KasI
Ksp632I KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 795 GGTACC G^GTACC
AlwNI 583 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 283 CCTAAGG CC^T[ACGT]AGG
Aor51HI 249 AGCGCT AGC^GCT
ApaI 53 GGGCCC GGGCC^C
Asp718I 795 GGTACC G^GTACC
AspHI 631 GAGCTC G[AT]GC[AT]^C
AvaI 1149 CTCGGG C^[CT]CG[GA]G
BamHI 1038 GGATCC G^GATCC
BanI 795 GGTACC G^G[CT][GA]CC
BbsI 132 GAAGAC GAAGAC(2/6)
BpuAI 132 GAAGAC GAAGAC(2/6)
BsaI 664 GAGACC GGTCTC(1/5)
BsgI 1376 GTGCAG GTGCAG(16/14)
BsiHKAI 631 GAGCTC G[AT]GC[AT]^C
BsiYI 149 CCAATATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 149 CCAATATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 886 GCATTC GAATGC(1/-1)
BsoBI 1149 CTCGGG C^[CT]CG[GA]G
BspHI 1137 TCATGA T^CATGA
BstXI 569 CCAAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 283 CCTAAGG CC^T[ACGT]AGG
DraII 53 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 249 AGCGCT AGC^GCT
Eco81I 283 CCTAAGG CC^T[ACGT]AGG
EcoO109I 53 GGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 249 AGCGCT [GA]GCGC^[CT]
HphI 404 TCACC GGTGA(8/7)
KpnI 795 GGTACC GGTAC^C
MaeII 136 ACGT A^CGT
MslI 44 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1070 CATATG CA^TATG
PshAI 273 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1137 TCATGA T^CATGA
SacI 631 GAGCTC GAGCT^C
SmlI 528 CTCAAG C^T[CT][GA]AG
TfiI 1183 GATTC G^A[AT]TC
XbaI 1089 TCTAGA T^CTAGA
XcmI 659 CCAGTGAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AvaII 272 GGACC G^G[AT]CC
1063 GGACC
BanII 53 GGGCCC G[GA]GC[CT]^C
631 GAGCTC
BglI 757 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1352 GCCAGTATGGC
BmyI 53 GGGCCC G[AGT]GC[ACT]^C
631 GAGCTC
BpmI 859 CTGGAG CTGGAG(16/14)
1382 CTGGAG
BsmAI 664 GAGAC GTCTC(1/5)
1032 GTCTC
Bsp1286I 53 GGGCCC G[AGT]GC[ACT]^C
631 GAGCTC
BsrDI 341 GCAATG GCAATG(2/0)
952 GCAATG
BssKI 811 CCAGG ^CC[ACGT]GG
1278 CCTGG
BstNI 811 CCAGG CC^[AT]GG
1278 CCTGG
BstYI 1038 GGATCC [GA]^GATC[CT]
1267 GGATCT
Cac8I 370 GCTGGC GC[ACGT]^[ACGT]GC
581 GCCAGC
CfoI 250 GCGC GCG^C
741 GCGC
DraI 25 TTTAAA TTT^AAA
1171 TTTAAA
EcoRII 811 CCAGG ^CC[AT]GG
1278 CCTGG
HhaI 250 GCGC GCG^C
741 GCGC
HinP1I 250 GCGC G^CGC
741 GCGC
HindIII 1142 AAGCTT A^AGCTT
1412 AAGCTT
MflI 1038 GGATCC [GA]^GATC[CT]
1267 GGATCT
MvaI 811 CCAGG CC^[AT]GG
1278 CCTGG
PleI 387 GACTC GAGTC(4/5)
779 GACTC
PstI 680 CTGCAG CTGCA^G
1305 CTGCAG
ScrFI 811 CCAGG CC^[ACGT]GG
1278 CCTGG
SfcI 680 CTGCAG C^T[GA][CT]AG
1305 CTGCAG
XhoII 1038 GGATCC [GA]^GATC[CT]
1267 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BalI BbvI BfaI BsaJI
BsmFI BspMI BsrI BstF5I Cfr13I
DdeI DpnII DsaI EaeI EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MwoI
NcoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I SfaNI StyI
Tru9I TseI Tsp45I Tsp509I TspRI