Restriction map for
pFN21AE5510
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
HindIII HpaI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MseI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 562 GTCTAC GT^[AC][GT]AC
AcsI 274 AAATTT [GA]^AATT[CT]
AhdI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 740 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 219 GGGCCC GGGCC^C
ApoI 274 AAATTT [GA]^AATT[CT]
AspEI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 545 TGCGCA TGC^GCA
BclI 455 TGATCA T^GATCA
BfaI 289 CTAG C^TAG
BglI 688 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 405 AGATCT A^GATCT
BsaI 867 GGTCTC GGTCTC(1/5)
BseRI 1240 GAGGAG GAGGAG(10/8)
BsmBI 149 GAGACG CGTCTC(1/5)
BspDI 1056 ATCGAT AT^CGAT
BspMI 68 ACCTGC ACCTGC(4/8)
BstAPI 1261 GCAATCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 405 AGATCT [GA]^GATC[CT]
ClaI 1056 ATCGAT AT^CGAT
DrdI 955 GACTTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 455 TGATCA T^GATCA
FspI 545 TGCGCA TGC^GCA
HapII 24 CCGG C^CGG
HpaII 24 CCGG C^CGG
MaeI 289 CTAG C^TAG
MflI 405 AGATCT [GA]^GATC[CT]
MspI 24 CCGG C^CGG
PflMI 939 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 380 GGGTCCC [GA]G^G[AT]CC[CT]
ScaI 211 AGTACT AGT^ACT
SmaI 23 CCCGGG CCC^GGG
SmlI 574 CTCAAG C^T[CT][GA]AG
StuI 1004 AGGCCT AGG^CCT
Tsp509I 275 AATT ^AATT
Van91I 939 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 405 AGATCT [GA]^GATC[CT]
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 553 GGCGCC G[GA]^CG[CT]C
1186 GGCGCC
AvaI 23 CCCGGG C^[CT]CG[GA]G
924 CTCGGG
BanI 553 GGCGCC G^G[CT][GA]CC
1186 GGCGCC
BbeI 553 GGCGCC GGCGC^C
1186 GGCGCC
BbsI 681 GAAGAC GAAGAC(2/6)
877 GAAGAC
BcnI 23 CCCGG CC^[GC]GG
24 CCGGG
BpuAI 681 GAAGAC GAAGAC(2/6)
877 GAAGAC
BsaHI 553 GGCGCC G[GA]^CG[CT]C
1186 GGCGCC
BsgI 70 CTGCAC GTGCAG(16/14)
1164 CTGCAC
BsoBI 23 CCCGGG C^[CT]CG[GA]G
924 CTCGGG
BspHI 503 TCATGA T^CATGA
1229 TCATGA
BsrBI 1113 CCGCTC CCGCTC(-3/-3)
1197 CCGCTC
BsrDI 1140 CATTGC GCAATG(2/0)
758 GCAATG
BstEII 703 GGTTACC G^GT[ACGT]ACC
1047 GGTGACC
BstPI 703 GGTTACC G^GT[ACGT]ACC
1047 GGTGACC
DsaI 53 CCACGG C^C[GA][CT]GG
119 CCACGG
EcoO65I 703 GGTTACC G^GT[ACGT]ACC
1047 GGTGACC
EcoT14I 80 CCAAGG C^C[AT][AT]GG
344 CCTTGG
FauI 799 CCCGC CCCGC(4/6)
361 GCGGG
HaeII 553 GGCGCC [GA]GCGC^[CT]
1186 GGCGCC
HgaI 1274 GACGC GACGC(5/10)
1206 GCGTC
Hin1I 553 GGCGCC G[GA]^CG[CT]C
1186 GGCGCC
HincII 268 GTTGAC GT[CT]^[GA]AC
1030 GTCAAC
HindII 268 GTTGAC GT[CT]^[GA]AC
1030 GTCAAC
KasI 553 GGCGCC G^GCGCC
1186 GGCGCC
MslI 612 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
729 CATGGATGTG
MspA1I 98 CAGCTG C[AC]G^C[GT]G
368 CAGCTG
NarI 553 GGCGCC GG^CGCC
1186 GGCGCC
NciI 23 CCCGG CC^[GC]GG
24 CCGGG
PleI 37 GACTC GAGTC(4/5)
182 GAGTC
PvuII 98 CAGCTG CAG^CTG
368 CAGCTG
RcaI 503 TCATGA T^CATGA
1229 TCATGA
SfaNI 607 GATGC GCATC(5/9)
742 GCATC
SfoI 553 GGCGCC GGC^GCC
1186 GGCGCC
StyI 80 CCAAGG C^C[AT][AT]GG
344 CCTTGG
Tsp45I 964 GTCAC ^GT[GC]AC
1048 GTGAC
XcmI 53 CCACGGTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
323 CCAATGCCATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanII BbvI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI TfiI
TseI TspRI TthHB8I