Restriction map for
pFN21AE1491
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AviII AvrII BamHI
BbrPI BciVI BfrI BglI BglII
BlnI BsaBI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BspDI BspHI BspLU11I BspMI BsrBI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MseI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 192 GACGTC GACGT^C
AccI 277 GTCTAC GT^[AC][GT]AC
AccIII 117 TCCGGA T^CCGGA
AhdI 187 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 978 GATCC GGATC(4/5)
AocI 8 CCTCAGG CC^T[ACGT]AGG
AspEI 187 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 49 GGCGCC GGCGC^C
BbsI 208 GAAGAC GAAGAC(2/6)
BbvCI 710 CCTCAGC CCTCAGC(-5/-2)
BcgI 435 CGAACTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 218 TGATCA T^GATCA
BlpI 789 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 789 GCTGAGC GC^T[ACGT]AGC
BpuAI 208 GAAGAC GAAGAC(2/6)
BsaAI 280 TACGTG [CT]AC^GT[GA]
BsaWI 117 TCCGGA [AT]^CCGG[AT]
BseAI 117 TCCGGA T^CCGGA
Bsp1407I 140 TGTACA T^GTACA
BspEI 117 TCCGGA T^CCGGA
BsrDI 358 CATTGC GCAATG(2/0)
BsrFI 523 GCCGGC [GA]^CCGG[CT]
BsrGI 140 TGTACA T^GTACA
BssHII 14 GCGCGC G^CGCGC
BstAPI 468 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 645 GGTCACC G^GT[ACGT]ACC
BstPI 645 GGTCACC G^GT[ACGT]ACC
BstXI 575 CCAGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 977 AGATCC [GA]^GATC[CT]
Bsu36I 8 CCTCAGG CC^T[ACGT]AGG
CelII 789 GCTGAGC GC^T[ACGT]AGC
Cfr10I 523 GCCGGC [GA]^CCGG[CT]
DsaI 968 CCGCGG C^C[GA][CT]GG
Eam1105I 187 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 8 CCTCAGG CC^T[ACGT]AGG
EcoO65I 645 GGTCACC G^GT[ACGT]ACC
EcoT14I 1037 CCTTGG C^C[AT][AT]GG
FbaI 218 TGATCA T^GATCA
HgaI 876 GACGC GACGC(5/10)
HincII 370 GTCAAC GT[CT]^[GA]AC
HindII 370 GTCAAC GT[CT]^[GA]AC
KasI 49 GGCGCC G^GCGCC
KspI 968 CCGCGG CCGC^GG
MflI 977 AGATCC [GA]^GATC[CT]
MroI 117 TCCGGA T^CCGGA
NaeI 523 GCCGGC GCC^GGC
NarI 49 GGCGCC GG^CGCC
NgoMIV 523 GCCGGC G^CCGGC
NspI 1022 GCATGT [GA]CATG^[CT]
PflMI 849 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 968 CCGCGG CCGC^GG
ScaI 62 AGTACT AGT^ACT
SfoI 49 GGCGCC GGC^GCC
SspBI 140 TGTACA T^GTACA
StyI 1037 CCTTGG C^C[AT][AT]GG
Van91I 849 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 56 CCAACAAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 977 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
ApaI 551 GGGCCC GGGCC^C
1056 GGGCCC
BfaI 448 CTAG C^TAG
719 CTAG
BsmFI 103 GGGAC GGGAC(10/14)
93 GTCCC
DpnII 219 GATC ^GATC
978 GATC
EarI 666 CTCTTC CTCTTC(1/4)
1198 GAAGAG
HaeII 49 GGCGCC [GA]GCGC^[CT]
923 AGCGCC
HphI 454 GGTGA GGTGA(8/7)
647 TCACC
Ksp632I 666 CTCTTC CTCTTC(1/4)
1198 GAAGAG
MaeI 448 CTAG C^TAG
719 CTAG
MaeIII 112 GTGAC ^GT[ACGT]AC
646 GTCAC
MboI 219 GATC ^GATC
978 GATC
NdeII 219 GATC ^GATC
978 GATC
PstI 466 CTGCAG CTGCA^G
1085 CTGCAG
Sau3AI 219 GATC ^GATC
978 GATC
SfcI 466 CTGCAG C^T[GA][CT]AG
1085 CTGCAG
Tsp45I 112 GTGAC ^GT[GC]AC
646 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI MaeII MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PvuII RsaI Sau96I ScrFI SfaNI
TseI TspRI