Restriction map for
pFN21AE9530
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Cac8I Cfr10I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 457 CGCG CG^CG
AocI 489 CCTCAGG CC^T[ACGT]AGG
Aor51HI 428 AGCGCT AGC^GCT
BalI 437 TGGCCA TGG^CCA
BamHI 494 GGATCC G^GATCC
BanI 479 GGCGCC G^G[CT][GA]CC
BbeI 479 GGCGCC GGCGC^C
BbvCI 402 CCTCAGC CCTCAGC(-5/-2)
BlpI 956 GCTCAGC GC^T[ACGT]AGC
BpmI 301 CTGGAG CTGGAG(16/14)
Bpu1102I 956 GCTCAGC GC^T[ACGT]AGC
BsaAI 433 TACGTG [CT]AC^GT[GA]
BspMI 1074 ACCTGC ACCTGC(4/8)
BsrDI 185 GCAATG GCAATG(2/0)
BstEII 654 GGTCACC G^GT[ACGT]ACC
BstPI 654 GGTCACC G^GT[ACGT]ACC
BstUI 457 CGCG CG^CG
Bsu36I 489 CCTCAGG CC^T[ACGT]AGG
CelII 956 GCTCAGC GC^T[ACGT]AGC
DraIII 1108 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 271 GACCTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 428 AGCGCT AGC^GCT
Eco81I 489 CCTCAGG CC^T[ACGT]AGG
EcoO65I 654 GGTCACC G^GT[ACGT]ACC
HgaI 231 GACGC GACGC(5/10)
HindIII 1224 AAGCTT A^AGCTT
KasI 479 GGCGCC G^GCGCC
MaeII 434 ACGT A^CGT
MaeIII 655 GTCAC ^GT[ACGT]AC
MluNI 437 TGGCCA TGG^CCA
MscI 437 TGGCCA TGG^CCA
MseI 1235 TTAA T^TAA
MvnI 457 CGCG CG^CG
NarI 479 GGCGCC GG^CGCC
NcoI 552 CCATGG C^CATGG
PflMI 1107 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 863 GACTC GAGTC(4/5)
PvuII 959 CAGCTG CAG^CTG
SacI 954 GAGCTC GAGCT^C
SapI 525 GCTCTTC GCTCTTC(1/4)
SfoI 479 GGCGCC GGC^GCC
SmlI 745 CTTGAG C^T[CT][GA]AG
SspI 769 AATATT AAT^ATT
Tru9I 1235 TTAA T^TAA
Tsp45I 655 GTCAC ^GT[GC]AC
Van91I 1107 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 231 GACGCC G[GA]^CG[CT]C
479 GGCGCC
Asp700I 13 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1086 GAACATCTTC
BbsI 228 GAAGAC GAAGAC(2/6)
940 GTCTTC
BcnI 476 CCCGG CC^[GC]GG
483 CCGGG
BfaI 52 CTAG C^TAG
1193 CTAG
BpuAI 228 GAAGAC GAAGAC(2/6)
940 GTCTTC
BsaHI 231 GACGCC G[GA]^CG[CT]C
479 GGCGCC
BsaI 379 GAGACC GGTCTC(1/5)
264 GGTCTC
DsaI 347 CCGTGG C^C[GA][CT]GG
552 CCATGG
EaeI 437 TGGCCA [CT]^GGCC[GA]
602 CGGCCA
EcoT14I 288 CCTTGG C^C[AT][AT]GG
552 CCATGG
HaeII 428 AGCGCT [GA]GCGC^[CT]
479 GGCGCC
HapII 477 CCGG C^CGG
483 CCGG
Hin1I 231 GACGCC G[GA]^CG[CT]C
479 GGCGCC
HincII 796 GTCAAC GT[CT]^[GA]AC
964 GTCAAC
HindII 796 GTCAAC GT[CT]^[GA]AC
964 GTCAAC
HpaII 477 CCGG C^CGG
483 CCGG
MaeI 52 CTAG C^TAG
1193 CTAG
MspA1I 959 CAGCTG C[AC]G^C[GT]G
1081 CAGCGG
MspI 477 CCGG C^CGG
483 CCGG
NciI 476 CCCGG CC^[GC]GG
483 CCGGG
PpuMI 583 GGGTCCC [GA]G^G[AT]CC[CT]
1134 AGGTCCC
StyI 288 CCTTGG C^C[AT][AT]GG
552 CCATGG
TfiI 207 GATTC G^A[AT]TC
895 GAATC
TspRI 696 CAGTG CA[GC]TG[ACGT][ACGT]^
1042 CAGTG
XcmI 413 CCACCGTCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
578 CCAATGGGTCCCTGG
XmnI 13 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1086 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI ApaI AspHI
AvaII BanII BbvI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BssKI BstF5I
BstNI BstYI CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp509I XhoII